The objective of this study was to evaluate the histopathological alterations in juvenile Penaeus vannamei caused by silver nanoparticles (AgNPs) for two types of experiments: at sublethal concentrations of 3.6 to 7.1 μg/μL of metallic silver (Ag) for a short period up to 72 h and for 2.6 to 7.9 μg of Ag/μL for the long period up to 264 h. The severity degree of the changes was evaluated and the histopathological index (Hi) was determined in both experiments using the necrosis (cellular dead) as an indicator. The pathological changes in the striated muscle, gills, antennal gland, circulatory system, heart, lymphoid organ, and connective tissue are described. The histopathological effects were similar for the two experiments without a direct relationship with the concentrations. In the short-term experiment, the values of Hi were higher (2.34 ± 0.41 at 48 hpi and 1.91 ± 0.39 at 72 hpi) compared with the long-term experiment (values between 0.57 ± 0.36 to 1.74 ± 0.57 at 264 hpi). The observed pathologies are similar to those caused by other metals, with the exception of the agglomerations of black particles in the gills, lymphoid organ, and muscle, which has not been previously reported. This work shows that silver nanoparticles cause damage to shrimp in sublethal concentrations.
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The datasets used and/or analysed during the current study are available from the corresponding author on reasonable request.
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The authors thank CONACyT for the support granted to the Basic Science Project number 258607 and Russian grand N18-29-24037. We also thank Tomsk Polytechnic University Competitiveness Enhancement Program, Project VIU-RS. Also, important recognition is given to the technicians Victor Joaquín Álvarez López and José Antonio Velázquez Garay, for their support during the experiments.
All sources of funding for the research were declared. The National Council for Science and Technology (CONACyT) granted MCCHS (CONACyT grant number 258607), while the Russian project N18-29-24037 supplied the silver nanoparticles of the project.
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All procedures were performed according to Mexican guidelines and policies stated in the NOM-062-ZOO-1999 (these guidelines apply mostly to mammalian species but we applied the same principles regarding animal welfare and care) and British guidelines for fish welfare reported by Ashley (2007).
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• Sublethal concentrations of silver nanoparticles caused 23% mortality to shrimp
• Silver nanoparticles at sublethal levels cause pathological damage to shrimp
• Silver nanoparticles produce necrosis to different tissues and organs
• The treated shrimp could mitigate through their immune system the effects of AgNps
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Chávez-Sánchez, MC., Abad-Rosales, S., Lozano-Olvera, R. et al. Silver nanoparticles induce histopathological alterations in juvenile Penaeus vannamei. Environ Sci Pollut Res 28, 8224–8234 (2021). https://doi.org/10.1007/s11356-020-11175-3
- Silver nanoparticles
- Pathological alterations
- Sublethal concentrations
- Penaeus vannamei