Abstract
The cation-independent bioflocculant (59LF) extracted from Klebsiella sp. 59L was characterized. 59LF consisted of protein (4.8%) and total sugar (85.2%) with high molecular weight (93.82% of 2120 kDa), and total sugar was composed of 76.45% of neutral sugar, 3.65% of uronic acid, and 1.43% of amino sugar. Results indicated that 59LF was pH tolerant and thermally stable, and the maximum yield of 59LF was 4.078 g/L after 48 h culture. The optimal flocculating activity for Kaolin particles was obtained when the dosage of 59LF was 7.0 mg/L without additional metal ions as coagulant aids. Furthermore, the surface properties of 59LF were observed using a Fourier-transform infrared spectrophotometer and X-ray photoelectron spectroscopy, whereas a porous structure was detected by a scanning electron microscope. Thus, a primary flocculation mechanism of 59LF was proposed. This study provided a potential cation-independent bioflocculant with high productivity and low dosage in future application.
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HL contributed to the study conception and design. Material preparation was performed by BW and YZ. Data collection and analysis, the first draft of the manuscript were written by KF. All authors read and approved the final manuscript.
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Fang, K., Wang, B., Zhang, Y. et al. Optimized production and characterization of cation-independent bioflocculant produced by Klebsiella sp. 59L. Environ Sci Pollut Res 28, 7981–7993 (2021). https://doi.org/10.1007/s11356-020-11162-8
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DOI: https://doi.org/10.1007/s11356-020-11162-8