Abstract
The use of sewage water as an irrigation source can be beneficial in agricultural practices, however, it may result in human health risks due to the consumption of heavy metal(loid)–contaminated food. This study evaluated the suitability of using sewage water (SW), freshwater (FW), and groundwater (GW) for vegetable irrigation in District Vehari. Spinach (Spinacia oleracea) plants were grown in pots irrigated with FW, GW, and SW in different proportions and combinations. The results indicated the substantial lesser buildup of heavy metal(loid)s (As (− 0.8%), Cd (− 38%), Cr (− 6.2%), Cu (− 20%), Fe (− 9.2%), Mn (− 13%), Ni (− 16%), Pb (− 19%), and Zn (−15%)) in soil after S. oleracea cultivation compared to unirrigated soil possibly due to high metal(loid) uptake by S. oleracea. Irrigation with all types of waters resulted in metal(loid) accumulation in S. oleracea predominantly in roots. The combinations of FW, GW, and SW resulted in high metal(loid) accumulation (As, Cd, Cr, Cu, Fe, Mn, Ni, Pb, and Zn) in the edible S. oleracea leaves than their alone application. Owing to high metal(loid) buildup, plants showed a linear trend in physiological imbalance in terms of reduced pigment content, induction of peroxidation, and oxidation of lipids. The severe oxidative stress was observed in S. oleracea plants under FW and GW irrigation due to high metal(loid) accumulation. The risk indices showed possible carcinogenic risk (CR > 0.0001) and non-carcinogenic risk (HI > 1) from the consumption of metal(loid)-contaminated S. oleracea leaves. Results revealed unsuitability of all waters and their combinations for S. oleracea irrigation. Moreover, this study does not encourage the use of mixed water for vegetable irrigation in Vehari District. Therefore, it is of utmost importance to monitor the quality of irrigation waters to ensure food safety and prevent chronic health risks to the exposed population.
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References
Ahmad K, Wajid K, Khan ZI, Ugulu I, Memoona H, Sana M, Nawaz K, Malik IS, Bashir H, Sher M (2019) Evaluation of potential toxic metals accumulation in wheat irrigated with wastewater. Bull Environ Contam Toxicol 102:822–828
Al Mahmud J, Bhuyan MB, Anee TI, Nahar K, Fujita M, Hasanuzzaman M (2019) Reactive oxygen species metabolism and antioxidant defense in plants under metal/metalloid stress, plant abiotic stress tolerance. Springer, pp 221–257
Antoniadis V, Shaheen S, Levizou E, Shahid M, Niazi N, Vithanage M, Ok Y, Bolan N, Rinklebe J (2019) A critical prospective analysis of the potential toxicity of trace element regulation limits in soils worldwide: are they protective concerning health risk assessment? - a review. Environ Int 127:819–847
Cardwell A, Hawker D, Greenway M (2002) Metal accumulation in aquatic macrophytes from Southeast Queensland, Australia. Chemosphere 48:653–663
Chaoua S, Boussaa S, El Gharmali A, Boumezzough A (2019) Impact of irrigation with wastewater on accumulation of heavy metals in soil and crops in the region of Marrakech in Morocco. J Saudi Soc Agric Sci 18:429–436
ESSA (2018) Environmental and social systems assessment. Wolrd Bank. PAKISTAN-Punjab Cities Program. documents.worldbank.org/curated/en/145731516802997998/pdf/122961-REVISED-ESSA-P156972-PUBLIC-Disclosed-5-4-2018.pdf
FAO (1992) Wastewater treatment and reuse in agriculture. Rome: Food and Agriculture Organization 1992
FAO (2020) Food and Agriculture Organization of the United Nations. http://www.fao.org/nr/water/aquastat/data/query/results.html On May 16, 2020
Fatima S, Hussain I, Rasool A, Xiao T, Farooqi A (2018) Comparison of two alluvial aquifers shows the probable role of river sediments on the release of arsenic in the groundwater of district Vehari, Punjab, Pakistan. Environ Earth Sci 77:382
Gonzaga MIS, Matias MIAS, Andrade KR, de Jesus AN, da Costa Cunha G, de Andrade RS, de Jesus Santos JC (2020) Aged biochar changed copper availability and distribution among soil fractions and influenced corn seed germination in a copper-contaminated soil. Chemosphere 240:124828
Hejna M, Moscatelli A, Stroppa N, Onelli E, Pilu S, Baldi A, Rossi L (2020) Bioaccumulation of heavy metals from wastewater through a Typha latifolia and Thelypteris palustris phytoremediation system. Chemosphere 241:125018
Hodges DM, DeLong JM, Forney CF, Prange RK (1999) Improving the thiobarbituric acid-reactive-substances assay for estimating lipid peroxidation in plant tissues containing anthocyanin and other interfering compounds. Planta 207:604–611
Hussain MI, Qureshi AS (2020) Health risks of heavy metal exposure and microbial contamination through consumption of vegetables irrigated with treated wastewater at Dubai, UAE. Environ Sci Pollut Res 1–14
Hussain A, Priyadarshi M, Dubey S (2019) Experimental study on accumulation of heavy metals in vegetables irrigated with treated wastewater. Appl Water Sci 9:122
Iqbal Z, Abbas F, Ibrahim M, Ayyaz MM, Ali S, Mahmood A (2019) Surveillance of heavy metals in maize grown with wastewater and their impacts on animal health in periurban areas of Multan, Pakistan. Pak J Agric Sci 56
Islam E, Liu D, Li T, Yang X, Jin X, Mahmood Q, Tian S, Li J (2008) Effect of Pb toxicity on leaf growth, physiology and ultrastructure in the two ecotypes of Elsholtzia argyi. J Hazard Mater 154:914–926
Jahany M, Rezapour S (2020) Assessment of the quality indices of soils irrigated with treated wastewater in a calcareous semi-arid environment. Ecol Indic 109:105800
Javed MT 2011 Mechanisms behind pH changes by plant roots and shoots caused by elevated concentration of toxic elements. Department of Botany, Stockholm University
Karimi A, Naghizadeh A, Biglari H, Peirovi R, Ghasemi A, Zarei A (2020) Assessment of human health risks and pollution index for heavy metals in farmlands irrigated by effluents of stabilization ponds. Environ Sci Pollut Res 1–11
Khalid S, Shahid M, Dumat C, Niazi NK, Bibi I, Gul Bakhat HFS, Abbas G, Murtaza B, Javeed HMR (2017) Influence of groundwater and wastewater irrigation on lead accumulation in soil and vegetables: implications for health risk assessment and phytoremediation. Int J Phytoremediation 19:1037–1046
Khalid S, Murtaza B, Shaheen I, Ahmad I, Ullah MI, Abbas T, Rehman F, Ashraf MR, Khalid S, Abbas S, Imran M (2018a) Assessment and public perception of drinking water quality and safety in district Vehari, Punjab, Pakistan. J Clean Prod 181:224–234
Khalid S, Shahid M, Natasha, Bibi I, Sarwar T, Shah A, Niazi NK (2018b) A review of environmental contamination and health risk assessment of wastewater use for crop irrigation with a focus on low and high-income countries. Int J Environ Res Public Health 15:895
Khan ZI, Ahmad K, Batool F, Wajid K, Mehmood N, Ashfaq A, Bashir H, Nadeem M, Ullah S (2019) Evaluation of toxic potential of metals in wheat crop grown in wastewater-contaminated soil in Punjab, Pakistan. Environ Sci Pollut Res 1–9
Lichtenthaler HK (1987): Chlorophylls and carotenoids: pigments of photosynthetic biomembranes. Methods Enzymol. Academic Press, pp 350–382
Lu A, Zhang S, Shan X-q (2005) Time effect on the fractionation of heavy metals in soils. Geoderma 125:225–234
Muhammad S, Shah MT, Khan S (2010) Arsenic health risk assessment in drinking water and source apportionment using multivariate statistical techniques in Kohistan region, northern Pakistan. Food Chem Toxicol 48:2855–2864
Murtaza B, Natasha, Amjad M, Shahid M, Imran M, Shah NS, Abbas G, Naeem MA (2019) Compositional and health risk assessment of drinking water from health facilities of District Vehari, Pakistan. Environ Geochem Health:1–13
Nascimento ECS, do Nascimento R, da Silva AAR, de Castro Bezerra CV, Batista MC, Veloso LLSA, de Araújo Pereira MC, Oliveira H (2019) Growth and photosynthetic pigments of cotton cultivars irrigated with saline water. Agric Sci 10:81–91
Natasha, Shahid M, Niazi NK, Khalid S, Murtaza B, Bibi I, Rashid MI (2018) A critical review of selenium biogeochemical behavior in soil-plant system with an inference to human health. Environ Pollut 234:915–934
Natasha, Shahid M, Dumat C, Khalid S, Rabbani F, Farooq ABU, Amjad M, Abbas G, Niazi NK (2019a) Foliar uptake of arsenic nanoparticles by spinach: an assessment of physiological and human health risk implications. Environ Sci Pollut Res 26:20121–20131
Natasha, Shahid M, Khalid S (2019b) Foliar application of lead and arsenic solutions to Spinacia oleracea: biophysiochemical analysis and risk assessment. Environ Sci Pollut Res https://doi.org/10.1007/s11356-019-06519-7
Natasha, Shahid M, Farooq ABU, Rabbani F, Khalid S, Dumat C (2020a) Risk assessment and biophysiochemical responses of spinach to foliar application of lead oxide nanoparticles: a multivariate analysis. Chemosphere 245:125605
Natasha, Shahid M, Khalid S (2020b) Foliar application of lead and arsenic solutions to Spinacia oleracea: biophysiochemical analysis and risk assessment. Environ Sci Pollut Res:1–11
Natasha, Shahid M, Khalid S, Bibi I, Bundschuh J, Niazi N, Dumat C (2020c) A critical review of mercury speciation, bioavailability, toxicity and detoxification in soil-plant environment: ecotoxicology and health risk assessment. Sci Total Environ 711:134749
Noori M, Mahdye M, Norozi R (2014) Effects of municipal wastewater irrigation on physiological and phytochemical parameters of Aegilops columnaris Zhuk (Poaceae=Graminae). Int J Res Agric Food Sci 1(4):1–9
Pinto FR, Mourato MP, Sales JR, Moreira IN, Martins LL (2017) Oxidative stress response in spinach plants induced by cadmium. J Plant Nutr 40:268–276
PMDFC (2011) Punjab Municipal Development Fund Company. Punjab municipal service improvement project. tmavehari.lgpunjab.org.pk › planning-report-vehari
Pourrut B, Shahid M, Dumat C, Winterton P, Pinelli E (2011) Lead uptake, toxicity, and detoxification in plants. In: Whitacre DM (ed) Reviews of environmental contamination and toxicology, vol 213. Springer New York, New York, pp 113–136
Rydzyński D, Piotrowicz-Cieślak A, Grajek H, Michalczyk D (2019) Chlorophyll degradation by tetracycline and cadmium in spinach (Spinacia oleracea L.) leaves. Int J Environ Sci Technol 16:6301–6314
Sarwar T, Shahid M, Natasha, Khalid S, Shah AH, Ahmad N, Naeem MA, ul Haq Z, Murtaza B, Bakhat HF (2019) Quantification and risk assessment of heavy metal build-up in soil–plant system after irrigation with untreated city wastewater in Vehari, Pakistan. Environ Geochem Health 1–17
Shah AH, Shahid M, Khalid S, Natasha, Shabbir Z, Bakhat HF, Murtaza B, Farooq A, Akram M, Shah GM, Nasim W, Niazi NK (2019) Assessment of arsenic exposure by drinking well water and associated carcinogenic risk in peri-urban areas of Vehari, Pakistan. Environ Geochem Health
Shahid M, Dumat C, Khalid S, Niazi NK, Antunes PM (2017) Cadmium bioavailability, uptake, toxicity and detoxification in soil-plant system. Rev Environ Contam Toxicol 241:73–137
Shahid M, Pinelli E, Dumat C (2018) Tracing trends in plant physiology and biochemistry: need of databases from genetic to kingdom level. Plant Physiol Biochem 127:630–635
Shehzad MT, Murtaza G, Shafeeque M, Sabir M, Nawaz H, Khan MJ (2019) Assessment of trace elements in urban topsoils of Rawalpindi-Pakistan: a principal component analysis approach. Environ Monit Assess 191:65
Slavković L, Škrbić B, Miljević N, Onjia A (2004) Principal component analysis of trace elements in industrial soils. Environ Chem Lett 2:105–108
Tchounwou PB, Yedjou CG, Patlolla AK, Sutton DJ (2012) Heavy metal toxicity and the environment, molecular, clinical and environmental toxicology. Springer, pp 133–164
WB-SCEA (2006) Pakistan Strategic Country Environmental Assessment, Report No 36946.World Bank report, 2008
WWF-Pakistan (2007) Pakistan’s water at risk. Freshwater & Toxics Programme, WWF, Pakistan
Xu J, Zhang J, Xie H, Li C, Bao N, Zhang C, Shi Q (2010) Physiological responses of Phragmites australis to wastewater with different chemical oxygen demands. Ecol Eng 36:1341–1347
Zubair M, Khan QU, Mirza N, Sarwar R, Khan AA, Baloch MS, Fahad S, Shah AN (2019) Physiological response of spinach to toxic heavy metal stress. Environ Sci Pollut Res 26:31667–31674
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Sardar, A., Shahid, M., Natasha et al. Risk assessment of heavy metal(loid)s via Spinacia oleracea ingestion after sewage water irrigation practices in Vehari District. Environ Sci Pollut Res 27, 39841–39851 (2020). https://doi.org/10.1007/s11356-020-09917-4
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DOI: https://doi.org/10.1007/s11356-020-09917-4