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Mycoremediation affects antioxidative status in winter rye plants grown at Chernobyl exclusion zone site in Ukraine

Abstract

Soil contaminated with heavy metals in general and radionuclides in particular represents an escalating problem for all living organisms. Since, Chernobyl nuclear power plant accident in 1986 in Ukraine, an exclusion zone of 30 km around the former power plant is uninhabitable land due to severe contamination. Two most notable beta emitters contributing to dose hazards for decades is radioactive 137Cs/90Sr. However, large parts of the zone are also highly contaminated with uranium particles (hot particles) bearing trace amounts of highly alpha-emitting radionuclides. We established an experiment at exclusion zone with the aim to investigate the influence of two macro fungi (Schizophyllum commune (S.C.) and Leucoagaricus naucinus (L.N.)) on oxidative status and antioxidative responses in winter rye plants; from this, we wanted to test the radionuclide/heavy metals retention capacity of both fungi, and probe their further potential for mycoremediation.

Result shows some differences in the concentrations of radionuclides/heavy metals and micro/macronutrients uptake in plants. As a biomarker of oxidative status, lipid peroxidation (LPO) levels and other antioxidative parameters were determined, i.e., superoxide-dismutase (SOD) isoenzymes, cysteine (CYS), and ascorbic acid (AA) concentrations as well as catalase (CAT) and glutathione reductase (GR) activities in winter rye shoots. LPO showed no significant differences between controls and plants cultivated with macro fungi. However, CAT activities were elevated in the presence of S.C/L.N compared with control, while GR activity was significantly higher only in presence of S.C. In contrast, isozyme of SOD (Cu,Zn-SOD) was the most prominent in control. Likewise, CYS content was lower in plants grown with both fungi, while AA concentration was only lower in the presence of L.N. The results showed that presence of fungi in radionuclide contaminated soil caused induction of antioxidative response in shoots of winter rye and that the response depended on the type of fungi used.

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Acknowledgments

The authors greatly acknowledge Dr. Johannes Raff of Institute for Resource Ecology, Helmholtz-Zentrum Dresden-Rossendorf (HZDR), Dresden, Germany, for providing two different macro fungi for the experiments. Authors also acknowledge Dr. Sergiy Dubchak, State Specialized Enterprise “Radon,” Kyiv, Ukraine, and Dr. Igor Chyzhevsky, State Specialized Enterprise “Eco-center,” Chornobyl, Ukraine, for their help during this experiment and in access of Chornobyl exclusion zone.

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Correspondence to Dharmendra K. Gupta.

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Vuković, A., Schulz, W., Čamagajevac, I.Š. et al. Mycoremediation affects antioxidative status in winter rye plants grown at Chernobyl exclusion zone site in Ukraine. Environ Sci Pollut Res 27, 25818–25827 (2020). https://doi.org/10.1007/s11356-020-09137-w

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  • DOI: https://doi.org/10.1007/s11356-020-09137-w

Keywords

  • Mycoremediation
  • Schizophyllum commune
  • Leucoagaricus naucinus
  • Chernobyl exclusion zone
  • Oxidative stress
  • Winter rye plant