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Exploring the distinctiveness of biomass and biomolecules from limnic microalgae of unexplored waters of Noyyal River, Western Ghats, for exploitation

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Abstract

Oleaginous microalgae with high biomass productivity, lipid content, and lipid productivity are desirable for sustainable biofuel production. Rapid and accurate quantification of lipid content facilitates the identification of promising microalgae candidates. In the present study, 23 freshwater microalgae species from river Noyyal were isolated and identified based on their morphological and molecular (18S rRNA) features and recorded as Karunya Algae Culture Collection (KACC). Their biomass and lipid content were characterized and screened using FT-IR, Nile red staining, and gravimetric method. Results generated from FT-IR spectra differentiated KACC microalgae based on their biochemical contents with Scenedesmus rubescens KACC 2 and Chlorococcum sp. KACC 13 possessed high total protein and lipid content, respectively. Nile red fluorescence at 530/575 nm showed the yellow fluorescence under a fluorescent microscope giving the evidence of high neutral lipids in 10 KACC microalgae isolates. Total lipid content showed prominent variation between the KACC isolates and found in the range of 4 to 32% of DW. Lipid productivity and biomass productivity showed a similar pattern among KACC strains. Thus, our findings serve as a baseline data on the bioprospecting potential of KACC isolates from river Noyyal, an unexplored area of Western Ghats.

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References

  • Abou-Shanab RAI, Hwang JH, Cho Y, Min B, Jeon BH (2011) Characterization of microalgal species isolated from fresh water bodies as a potential source for biodiesel production. Appl Energy 88:3300–3306

    Article  CAS  Google Scholar 

  • Ahmed F, Fanning K, Netzel M, Turner W, Li Y, Schenk PM (2014) Profiling of carotenoids and antioxidant capacity of microalgae from subtropical coastal and brackish waters. Food Chem 165:300–306

    Article  CAS  Google Scholar 

  • Amit, Ghosh UK (2018) An approach for phycoremediation of different wastewaters and biodiesel production using microalgae. Environ Sci Pollut Res 25(19):18673–18681

    Article  CAS  Google Scholar 

  • Bligh EG, Dyer WM (1959) A rapid method of lipid extraction and purification. Can J Biochem Physiol 37:911–917

    Article  CAS  Google Scholar 

  • Bogen C, Klassen V, Wichmann J, La Russa M, Doebbe A, Grundmann M, Uronen P, Kruse O, Mussgnug JH (2013) Identification of Monoraphidium contortum as a promising species for liquid biofuel production. Bioresour Technol 133:622–626

    Article  CAS  Google Scholar 

  • Budhi Venkatesan R, Rajarathinam R (2019) Screening of microalgae for treating Garcinia cambogia wash water with potential lipid production. Environ Sci Pollut Res 26(34):34685–34692

    Article  CAS  Google Scholar 

  • Chen W, Zhang C, Song L, Sommerfeld M, Hu QA (2009) High throughput Nile red method for quantitative measurement of neutral lipids in microalgae. J Microbiol Methods 77:41–47

    Article  CAS  Google Scholar 

  • Chisti Y (2007) Biodiesel from microalgae. Biotechnol Adv 25:294–306

    Article  CAS  Google Scholar 

  • Cobelas MA, Lechado JZ (1989) Lipids in microalgae. A review. I. Biochemistry. Grasasy Aceites 40:118–145

    Google Scholar 

  • Dean AP, Sigee DC, Estrada B, Pittman JK (2010) Using FTIR spectroscopy for rapid determination of lipid accumulation in response to nitrogen limitation in freshwater microalgae. Bioresour Technol 101:4499–4507

    Article  CAS  Google Scholar 

  • Doyle JJ, Doyle JL (1987) A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochem Bull 19:11–15

    Google Scholar 

  • Ghasemi Naghdi F, Thomas-Hall SR, Durairatnam R, Pratt S, Schenk PM (2014) Comparative effects of biomass pre-treatments for direct and indirect transesterification to enhance microalgal lipid recovery. Front Energy Res 2:57

    Article  Google Scholar 

  • Giordano M, Kansiz M, Heraud P, Beardall J, Wood B, McNaughton D (2001) Fourier transform infrared spectroscopy as a novel tool to investigate changes in intracellular macromolecular pools in the marine microalga Chaetoceros muellerii (Bacillariophyceae). J Phycol 37:271–279

    Article  CAS  Google Scholar 

  • Giordano M, Ratti S, Domenighini A, Vogt F (2009) Spectroscopic classification of 14 different microalga species: first steps towards spectroscopic measurement of phytoplankton biodiversity. Plant Ecol Divers 2:155–164

    Article  Google Scholar 

  • Griffiths MJ, Harrison STL (2009) Lipid productivity as a key characteristic for choosing algal species for biodiesel production. J Appl Phycol 21:493–507

    Article  CAS  Google Scholar 

  • Gumbi ST, Majeke BM, Olaniran AO, Mutanda T (2017) Isolation, identification and high-throughput screening of neutral lipid producing indigenous microalgae from South African aquatic habitats. Appl Biochem Biotechnol 182:382–399

    Article  CAS  Google Scholar 

  • Ichihara K, Fukubayashi Y (2010) Preparation of fatty acid methyl esters for gasliquid chromatography. J Lipid Res 51(3):635–640

    Article  CAS  Google Scholar 

  • Kakarla R, Choi JW, Yun JH, Kim BH, Heo J, Lee S, Cho DH, Ramanam R, Kim HS (2018) Application of high-salinity stress for enhancing the lipid productivity of Chlorella sorokiniana HS1 in a two-phase process. J Microbiol 56:56–64

    Article  CAS  Google Scholar 

  • Kanaga K, Pandey A, Kumar S, Geetanjali (2016) Multi-objective optimization of media nutrients for enhanced production of algae biomass and fatty acid biosynthesis from Chlorella pyrenoidosa NCIM 2738. Bioresour Technol 200:940–950

    Article  CAS  Google Scholar 

  • Karpagam R, Jawahar K, Ashokkumar B, Varalakshmi P (2015) Characterization and fatty acid profiling in two fresh water microalgae for biodiesel production: lipid enhancement methods and media optimization using response surface methodology. Bioresour Technol 188:177–184

    Article  CAS  Google Scholar 

  • Krzeminska I, Piasecka A, Nosalewicz A, Simionato D, Wawrzykowski J (2015) Alterations of the lipid content and fatty acid profile of Chlorella protothecoides under different light intensities. Bioresour Technol 196:72–77

    Article  CAS  Google Scholar 

  • Kumar PS, Thomas J (2019) Seasonal distribution and population dynamics of limnic microalgae and their association with physico-chemical parameters of river Noyyal through multivariate statistical analysis. Sci Rep 9(1):15021

    Article  CAS  Google Scholar 

  • Li Q, Du W, Liu D (2008a) Perspectives of microbial oils for biodiesel production. Appl Microbiol Biotechnol 80:749–756

    Article  CAS  Google Scholar 

  • Li Y, Horsman M, Wu N, Lan CQ, Dubois-Calero N (2008b) Biofuels from microalgae. Biotechnol Prog 24:815–820

    CAS  Google Scholar 

  • Li L, Cui J, Lui Q, Ding Y, Lui J (2015) Screening and phylogenetic analysis of lipid-rich microalgae. Algal Res 11:381–386

    Article  Google Scholar 

  • Limayem A, Micciche A, Nayak B, Mohapatra S (2018) Prokaryotic community profiling of local algae wastewaters using advanced 16S rRNA gene sequencing. Environ Sci Pollut Res 25(1):704–711

    Article  CAS  Google Scholar 

  • Ma Y, Wang Z, Yu C, Yin Y, Zhou G (2014) Evaluation of the potential of 9 Nannochloropsis strains for biodiesel production. Bioresour Technol 167:503–509

    Article  CAS  Google Scholar 

  • Mandotra SK, Kumar P, Suseela MR, Ramteke PW (2014) Fresh water green microalga Scenedesmus abundans: a potential feedstock for high quality biodiesel production. Bioresour Technol 156:42–47

    Article  CAS  Google Scholar 

  • Mata TM, Martins AA, Caetano NS (2010) Microalgae for biodiesel production and other applications: a review. Renew Sust Energ Rev 14:217–232

    Article  CAS  Google Scholar 

  • Meng Y, Yao C, Xue S, Yang H (2014) Application of fourier transform infrared (FT-IR) spectroscopy in determination of microalgal compositions. Bioresour Technol 151:347–354

    Article  CAS  Google Scholar 

  • Murdock JN, Wetzel DL (2009) FT-IR microspectroscopy enhances biological and ecological analysis of algae. Appl Spectrosc Rev 44:335–361

    Article  CAS  Google Scholar 

  • Ordog V, Stirk WA, Balint P, Aremu AO, Okem A, Lovazs C, Molnár Z, van Staden J (2016) Effect of temperature and nitrogen concentration on lipid productivity and fatty acid composition in three Chlorella strains. Algal Res 16:141–149

    Article  Google Scholar 

  • Papazi A, Makridis P, Divanach P (2010) Harvesting Chlorella minutissima using cell coagulants. J Appl Phycol 22:349–355

    Article  CAS  Google Scholar 

  • Piligaev AV, Sorokina KN, Bryanskaya AV, Peltek SE, Kolchanov NA, Parmon VN (2015) Isolation of prospective microalgal strains with high saturated fatty acid content for biofuel production. Algal Res 12:368–376

    Article  Google Scholar 

  • Pistorius M, Degrip WJ, Egorova-Zachernyuk TA (2009) Monitoring of biomass composition from microbiological sources by means of FT-IR spectroscopy. Biotechnol Bioeng 103(1):123–129

    Article  CAS  Google Scholar 

  • Qu Z, Duan P, Cao X, Liu M, Lin L, Li M (2019) Comparison of monoculture and mixed culture (Scenedesmus obliquus and wild algae) for C, N, and P removal and lipid production. Environ Sci Pollut Res 26(20):20961–20968

    Article  CAS  Google Scholar 

  • Ra CH, Kang CH, Kim NK, Lee CG, Kim SK (2015) Cultivation of four microalgae for biomass and oil production using a two-stage culture strategy with salt stress. Renew Energy 80:117–122

    Article  CAS  Google Scholar 

  • Rawat I, Kumar RR, Mutanda T, Bux F (2013) Biodiesel from microalgae: a critical evaluation from laboratory to large scale production. Appl Energy 103:444–467

    Article  CAS  Google Scholar 

  • Sajjadi B, Chen WY, Raman AAA, Ibrahim S (2018) Microalgae lipid and biomass for biofuel production: a comprehensive review on lipid enhancement strategies and their effects on fatty acid composition. Renew Sust Energ Rev 97:200–232

    Article  CAS  Google Scholar 

  • Selvarajan R, Felfoldi T, Tauber T, Sanniyasi E, Sibanda T, Tekere M (2015) Screening and evaluation of some green algal strains (Chlorophyceae) isolated from freshwater and soda lakes for biofuel production. Energies 8:7502–7521

    Article  Google Scholar 

  • Sharma K, Schuhmann H, Schenk PM (2012) High lipid induction in microalgae for biodiesel production. Energies 5:1532–1553

    Article  CAS  Google Scholar 

  • Spolaore P, Joannis-Cassan C, Duran E, Isambert A (2006) Commercial applications of microalgae. J Biosci Bioeng 101:87–96

    Article  CAS  Google Scholar 

  • Stehfest K, Toepel J, Wilhelm C (2005) The application of micro-FTIR spectroscopy to analyze nutrient stress-related changes in biomass composition of phytoplankton algae. Plant Physiol Biochem 43:717–726

  • Sukacova K, Buzova D, Travnicek P, Cerveny J, Vítezova M, Vítez T (2019) Optimization of microalgal growth and cultivation parameters for increasing bioenergy potential : case study using the oleaginous microalga Chlorella. Algal Res 40:101519

  • Sureshkumar P, Thomas J (2019) Strategic growth of limnic green microalgae with phycoremediation potential for enhanced production of biomass and biomolecules for sustainable environment. Environ Sci Pollut Res (In press) 26:34702–34712. https://doi.org/10.1007/s11356-018-4012-9

    Article  CAS  Google Scholar 

  • SureshKumar P, Thomas J, SivaSubramanian J (2017) Rapid method for simultaneous discrimination of microalgae and determination on biochemical composition based on vibrational spectroscopy. Int J Algae 19(4):385–396

    Article  Google Scholar 

  • Tabatabai B, Chen H, Lu J, Giwa-Otusajo J, McKenna AM, Shrivastava AK, Sitther V (2018) Fremyella diplosiphon as a biodiesel agent: identification of fatty acid methyl esters via microwave-assisted direct in situ transesterification. Bioenerg Res 11(3):528–537

    Article  CAS  Google Scholar 

  • Tamura K, Dudley J, Nei M, Kumar S (2007) MEGA4: molecular evolutionary genetics analysis (MEGA) software version 4.0. Mol Biol Evol 24:1596–1599

    Article  CAS  Google Scholar 

  • Valdez-ojeda R, González-muñoz M, Us-vázquez R, Narváez-zapata J, Chavarria-hernandez JC, López-adrián S, Barahona-pérez F, Toledano-thompson T, Garduño-solórzano G, María R, Medrano E (2015) Characterization of five fresh water microalgae with potential for biodiesel production. Algal Res 7:33–44

    Article  Google Scholar 

  • Wagner H, Liu Z, Langner U, Stehfest K, Wilhelm C (2010) The use of FTIR spectroscopy to assess quantitative changes in the biochemical composition of microalgae. J Biophotonics 3:557–566

    Article  CAS  Google Scholar 

  • Zhang C, Zhang Y, Zhuang B, Zhou X (2014) Strategic enhancement of algal biomass, nutrient uptake and lipid through statistical optimization of nutrient supplementation in coupling Scenedesmus obliquus-like microalgae cultivation and municipal wastewater treatment. Bioresour Technol 171:71–79

    Article  CAS  Google Scholar 

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Acknowledgements

The author thanks the Karunya Institute of Technology and Sciences for providing the research facilities.

Funding

The authors gratefully acknowledge the financial support funded by the Science and Engineering Research Board–Department of Science and Technology (SERB–DST), Government of India (S.O: No/SB/FT/LS-389/2012).

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Authors and Affiliations

  1. Algae Biomass Research Laboratory, Department of Biotechnology, School of Agriculture and Biosciences, Karunya Institute of Technology and Sciences, Coimbatore, Tamil Nadu, 641 114, India

    Pandian Sureshkumar & Jibu Thomas

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  1. Pandian Sureshkumar
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  2. Jibu Thomas
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PS and JT conceived the research and designed the experiments. PS performed the experiments, and analyzed and interpreted the data. PS wrote the original draft. JT supervised and edited the manuscript. All authors edited and approved the final manuscript.

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Correspondence to Jibu Thomas.

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Sureshkumar, P., Thomas, J. Exploring the distinctiveness of biomass and biomolecules from limnic microalgae of unexplored waters of Noyyal River, Western Ghats, for exploitation. Environ Sci Pollut Res 27, 23309–23322 (2020). https://doi.org/10.1007/s11356-020-08921-y

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