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The association between the diesel exhaust particle exposure from bus emission and the tubular epithelial cell deformation of rats

Environmental Science and Pollution Research volume 27, pages 23073–23080 (2020)Cite this article

Abstract

The diesel vehicle emissions regarding particles have become a problem due to human health adversely. Especially ultrafine particles (diameter ≤ 100 nm) can deeply penetrate the human body leading to cell deformation. Investigation of the diesel ultrafine particle exposure to the cell deformation has become a challenge to build up understanding the impacts of ultrafine particles on human health. Moreover, the relationship between high exposure to diesel ultrafine particles and the deformation of the rat’s tubular epithelial cells is not clear. In this study, we investigated the impact of the diesel ultrafine particle exposure to the rat’s tubular cells. Three diesel busses were used as the sources of the particles, while 50 rats were used as the experimental animals. The diesel emission was filtered using an N95 particulate filter and a suction pump. The rats were exposed to the diesel ultrafine particle emission for 100 s with three different concentrations C1, C2, and C3 for eight consecutive days. All rats were sacrificed on the day after exposures to examine the histological images. The results showed that the deformation level of the tubular epithelial cells was positively associated with the concentration of the ultrafine particles.

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Acknowledgments

The authors wish to acknowledge Arif Budianto, Eko TP Adi, Mia A Pawestri, and Arsyal K Rumpoko for their excellent technical assistance.

Funding

This study was supported by the Indonesian Ministry of Research, Technology, and Higher Education [contract numbers 033/SP2H/LT/DRPM/II/2016 and 137/SP2H/LT/DRPM/II/2016].

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Affiliations

  1. Laboratory of Air Quality and Astro Imaging, Physics Department, Brawijaya University, Jl. Veteran 65145, Malang, Indonesia

    Arinto Yudi Ponco Wardoyo

  2. Laboratory of Biophysics, Physics Department, Brawijaya University, Jl. Veteran 65145, Malang, Indonesia

    Unggul Pundjung Juswono & Johan Andoyo Effendi Noor

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  1. Arinto Yudi Ponco Wardoyo
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  2. Unggul Pundjung Juswono
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  3. Johan Andoyo Effendi Noor
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Correspondence to Arinto Yudi Ponco Wardoyo.

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Wardoyo, A.Y.P., Juswono, U.P. & Noor, J.A.E. The association between the diesel exhaust particle exposure from bus emission and the tubular epithelial cell deformation of rats. Environ Sci Pollut Res 27, 23073–23080 (2020). https://doi.org/10.1007/s11356-020-08752-x

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  • DOI: https://doi.org/10.1007/s11356-020-08752-x

Keywords

  • Bus emission
  • Diesel engine
  • Ultrafine particles
  • Tubular epithelial cell
  • Deformation
  • Rat’s kidney