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Orthosilicic acid (OSA) reduced grain arsenic accumulation and enhanced yield by modulating the level of trace element, antioxidants, and thiols in rice

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Abstract

Arsenic (As), a toxic metalloid, is finding its route to human through intake of As-contaminated water and consumption of food grown on contaminated soil. Rice is the most As-affected crop. Present study is aimed to assess the impact of stabilized orthosilicic acid (a proprietary formulation for plant-available silicon (Si) and earlier used as fertilizer for rice to enhance growth and yield) in reducing the accumulation of As in rice grains. Application of arsenic in the form of arsenate (AsV) and arsenite (AsIII) significantly affected plant growth in a dose-dependent manner. Higher doses of AsV and AsIII (50 and 25 mg L−1 respectively) significantly decreased the yield attributes leading to lower yield. A significant accumulation of As in grain was observed in both AsV- and AsIII-exposed plants in a dose-dependent manner. Arsenic exposure also increased the level of Si in rice grains. Application of Si, either in soil or on leaves (foliar), greatly reduced grain As accumulation (up to 67% in AsV and 78% in AsIII) and enhanced the growth and yield of plants under As stress. The level of thiols and activities of antioxidant enzymes were also enhanced under Si application. Foliar Si application was more effective in increasing grain Si level and reducing grain As than soil Si. The level of other trace elements was also significantly enhanced by Si application irrespective of the presence or absence of As in comparison with control. Arsenic exposure constrained some of the trace elements, such as Zn and Co, which were restored by Si application. Results of the present study showed that the application of currently used Si formulation may effectively reduce grain As level even in highly As-contaminated soil and improve grain quality of rice.

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Acknowledgments

Authors are thankful to Director, CSIR-National Botanical Research Institute, Lucknow, for providing the infrastructure and laboratory facilities. SERB-DST and CSIR, New Delhi, are acknowledged for the award of Young Scientist Fellowship (SB/YS/LS-381/2013) and SRA (letter No. IA-27594, dated 02-11-2017) respectively to SM.

Funding

Privi Life Science Pvt. Ltd., Mumbai, is also acknowledged for financial support for this study.

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Author notes

  1. Sanjay Dwivedi and Amit Kumar contributed equally to this work.

Authors and Affiliations

  1. Plant Ecology and Environmental Science Division, CSIR-National Botanical Research Institute, Lucknow, 226001, India

    Sanjay Dwivedi, Amit Kumar, Seema Mishra, Pragya Sharma, Geetgovind Sinam & Rudra Deo Tripathi

  2. Deen Dayal Upadhyaya Gorakhpur University, Gorakhpur, 273009, India

    Seema Mishra

  3. Soil Science Laboratory, CSIR-National Botanical Research Institute, Lucknow, 226001, India

    Lal Bahadur

  4. Privi Life Science Private Limited, Mumbai, India

    Vinod Goyal & Neeru Jain

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  1. Sanjay Dwivedi
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Correspondence to Seema Mishra or Rudra Deo Tripathi.

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Dwivedi, S., Kumar, A., Mishra, S. et al. Orthosilicic acid (OSA) reduced grain arsenic accumulation and enhanced yield by modulating the level of trace element, antioxidants, and thiols in rice. Environ Sci Pollut Res 27, 24025–24038 (2020). https://doi.org/10.1007/s11356-020-08663-x

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