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Effects of mercury binding by humic acid and humic acid resistance on mercury stress in rice plants under high Hg/humic acid concentration ratios

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Abstract

Due to the nonsystematic nature of previous studies on mercury (Hg) mobility with humic substances (HS) in terrestrial ecosystems and the uncertainty of Hg accumulation in plants, oxygen-rich humic acid (HA), which is the main component of HS, was used as the target in this study. Batch sorption tests and a series of pot experiments were designed to investigate the effect of HS on Hg binding and therefore Hg uptake in rice plants under extreme conditions, i.e., a high Hg/HS concentration ratio. The results showed that HA was eligible for Hg binding, though it has a tiny proportion of sulfur according to its characteristics analysis. The binding of HA and Hg was a chemisorption process in a single layer that followed the pseudo-second order and Langmuir models, and it was also verified that the pH was dependent on the ion strength associated with high Hg/HA concentration ratios. Based on the pot experiments, the performance of HA with Hg was investigated. The Hg in the toxicity characteristic leaching procedure (TCLP) leachate under high Hg/HA concentration ratios declined significantly, and accordingly, all treatments met the concentration criteria of 0.1 mg/l (GB 5085.3–2007) for wastes after 30 days of exposure. At concentration ratios of 50, 25, and 10 μg Hg/mg HA, we observed that HA application promoted rice plant growth, as reflected in the increase of fresh weight of different organs. Regarding accumulation in the soil-plant system, the degradation of HA to smaller molecules by rhizosphere microorganisms and organic acids in roots made HA available for plant uptake through the vascular bundle in roots, thus promoting Hg transformation in plants to a certain extent. However, considering the decline in available Hg in the soil, the Hg concentrations of roots, straw, and grains in the ripening stage were found to be lower than those in the standalone Hg treatments. HA clearly has a direct effect on Hg and an indirect influence on plants exposed to Hg under extreme conditions (very high Hg/HA concentration ratios); thus, the biogeochemical behavior of Hg at high Hg/HA concentration ratios should be considered and further investigated.

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References

  • Amirbahman A, Reid A, Haines T, Kahl J, Arnold C (2002) Association of methylmercury with dissolved humic acids. Environ Sci Technol 36:690–695

    CAS  Google Scholar 

  • Barnie S, Zhang J, Wang H, Yin HL, Chen HH (2018) The influence of pH, co-existing ions, ionic strength, and temperature on the adsorption and reduction of hexavalent chromium by undissolved humic acid. Chemosphere 212:209–218. https://doi.org/10.1016/j.chemosphere.2018.08.067

    Article  CAS  Google Scholar 

  • Cattani I, Zhang H, Beone GM, Del Re AA, Boccelli R, Trevisan M (2009) The role of natural purified humic acids in modifying mercury accessibility in water and soil. J Environ Qual 38:493–501. https://doi.org/10.2134/jeq2008.0175

    Article  CAS  Google Scholar 

  • Cerdán M, Sánchez-Sánchez A, Jordá JD, Juárez M, Sánchez-Andreu J (2013) Effect of commercial amino acids on iron nutrition of tomato plants grown under lime-induced iron deficiency. J Plant Nutr Soil Sci 176:859–866. https://doi.org/10.1002/jpln.201200525

    Article  CAS  Google Scholar 

  • Chai X, Liu G, Zhao X, Hao Y, Zhao Y (2012) Complexion between mercury and humic substances from different landfill stabilization processes and its implication for the environment. J Hazard Mater 209-210:59–66. https://doi.org/10.1016/j.jhazmat.2011.12.077

    Article  CAS  Google Scholar 

  • Chakraborty P, Sarkar A, Vudamala K, Naik R, Nath BN (2015) Organic matter — a key factor in controlling mercury distribution in estuarine sediment. Marine Chemistry 173:302–309. https://doi.org/10.1016/j.marchem.2014.10.005

    Article  CAS  Google Scholar 

  • Chen J, Gu B, Leboeuf EJ, Pan H, Dai S (2002) Spectroscopic characterization of the structural and functional properties of natural organic matter fractions. Chemosphere 48:59–68

    CAS  Google Scholar 

  • Chen SC, Lin WH, Chien CC, Tsang D, Kao CM (2018) Development of a two-stage biotransformation system for mercury-contaminated soil remediation. Chemosphere 200:266–273

    CAS  Google Scholar 

  • De P. Santos AM, Bertoli AC, Duarte HA, Garcia JS, Trevisan MG (2019) The stability and mechanism of cerium complexation with humic substances from poultry manure – a combined experimental/theoretical approach. J Mol Struct 1178:290–297. https://doi.org/10.1016/j.molstruc.2018.10.045

    Article  CAS  Google Scholar 

  • Dong C, Wei C, Cheng L, Yu L, Liu H (2014) Synthesis of magnetic chitosan nanoparticle and its adsorption property for humic acid from aqueous solution. Colloids Surf A Physicochem Eng Asp 446:179–189

    CAS  Google Scholar 

  • Dorota K, Ewa K (2011) Organic matter transformations and kinetics during sewage sludge composting in a two-stage system. Bioresour Technol 102:10951–10958

    Google Scholar 

  • Drexel RT, Haitzer M, Ryan JN, Aiken GR, Nagy KL (2002) Mercury (II) sorption to two Florida Everglades peats: Evidence for strong and weak binding and competition by dissolved organic matter released from the peat. Environ Sci Technol 36:4058–4064

    CAS  Google Scholar 

  • Enev V, Pospíšilová L, Klučáková M, Liptaj T, Doskočil L (2014) Spectral characterization of selected humic substances. Soil Water Res 9:9–17

    CAS  Google Scholar 

  • Esteves VI, Otero M, Duarte AC (2009) Comparative characterization of humic substances from the open ocean, estuarine water and fresh water. Org Geochem 40:942–950

    CAS  Google Scholar 

  • Felbeck GT, Felbeck GT, Felbeck GT (1975) Humic substances in the environment soil. Sci Soc Am J 39:130

    Google Scholar 

  • Feng X, Li P, Qiu G, Wang S, Li G, Shang L, Meng B, Jiang H, Bai W, Li Z, Fu X (2008) Human exposure to methylmercury through rice intake in mercury mining areas. Environ Sci Technol 42:326–332

    CAS  Google Scholar 

  • Fernandes AN, Giovanela M, Esteves VI, MMDS S (2010) Elemental and spectral properties of peat and soil samples and their respective humic substances. J Mol Struct 971:33–38

    CAS  Google Scholar 

  • Giovanela M, Crespo JS, Antunes M, Adamatti DS, Fernandes AN, Barison A (2010) Chemical and spectroscopic characterization of humic acids extracted from the bottom sediments of a Brazilian subtropical microbasin. J Mol Struct 981:111–119

    CAS  Google Scholar 

  • Haitzer M, Aiken G, Ryan J (2002) Binding of mercury(II) to dissolved organic matter. Environ Sci Technol 36:3564–3570

    CAS  Google Scholar 

  • He E, Lü C, He J, Zhao B, Wang J, Zhang R, Ding TJES (2016) Binding characteristics of cu 2+ to natural humic acid fractions sequentially extracted from the lake sediments. Environ Sci Pollut Res 23:22667–22677

    CAS  Google Scholar 

  • Hesterberg D, Chou J, Hutchison K, Sayers D (2001) Bonding of Hg (II) to reduced organic sulfur in humic acid as affected by S/Hg ratio. Environ Sci Technol 35:2741–2745

    CAS  Google Scholar 

  • Horuz A, Karaman MR, Gulluce M (2015) Effect of humic acid application on the reduction of cadmium concentration in lettuce (Lactuca sativa L). Fresenius Environ Bull 24:3141–3147

    CAS  Google Scholar 

  • Krishnamurti G, Naidu R (2002) Solid-solution speciation and phytoavailability of copper and zinc in soils. Environ Sci Technol 36:2645–2651

    CAS  Google Scholar 

  • Kwiatkowska-Malina J (2015) Polish J Soil Sci 48:57–64. https://doi.org/10.17951/pjss/2015.48.1.57

    Article  Google Scholar 

  • Li Y et al (2017) Influence of sulfur on the accumulation of mercury in rice plant (Oryza sativa L.) growing in mercury contaminated soils. Chemosphere 182:293–300. https://doi.org/10.1016/j.chemosphere.2017.04.129

    Article  CAS  Google Scholar 

  • Li Y et al (2018) Thiosulfate amendment reduces mercury accumulation in rice (Oryza sativa L.). Plant Soil 430:413–422. https://doi.org/10.1007/s11104-018-3726-2

    Article  CAS  Google Scholar 

  • Liu G, Cai Y (2010) Complexation of arsenite with dissolved organic matter: conditional distribution coefficients and apparent stability constants. Chemosphere 81:890–896

    CAS  Google Scholar 

  • Markus H, Aiken GR, Ryan JN (2002) Binding of mercury(II) to dissolved organic matter: the role of the mercury-to-DOM concentration ratio. Environ Sci Technol 36:3564–3570

    Google Scholar 

  • Marrugonegrete J, Marrugomadrid S, Pinedohernández J, Durangohernández J, Díez S (2016) Screening of native plant species for phytoremediation potential at a Hg-contaminated mining site. Sci the Total Environ 542:809–816

    CAS  Google Scholar 

  • Mecozzi M, Amici M, Pietrantonio E, Romanelli G (2002) An ultrasound assisted extraction of the available humic substance from marine sediments. Ultrason Sonochem 9:11–18

    CAS  Google Scholar 

  • O’Connor D et al (2018) Sulfur-modified rice husk biochar: a green method for the remediation of mercury contaminated soil. Sci Total Environ 621:819–826

    Google Scholar 

  • Pearson RG (1969) Hard and soft acids and bases. Surv Prog Chem 5:1–52

    CAS  Google Scholar 

  • Perminova IV, Frimmel FH, Kudryavtsev AV, Kulikova NA, Gudrun AB, Sebastian H, Petrosyant VS (2003) Molecular weight characteristics of humic substances from different environments as determined by size exclusion chromatography and their statistical evaluation. Environ Sci Technol 37:2477–2485

    CAS  Google Scholar 

  • Qi G, Yue D, Fukushima M, Fukuchi S, Nishimoto R, Nie Y (2012) Enhanced humification by carbonated basic oxygen furnace steel slag – II. Process characterization and the role of inorganic components in the formation of humic-like substances. Bioresourc Technol 114:637–643

    CAS  Google Scholar 

  • Qiu G, Feng X, Wang S, Shang L (2005) Mercury and methylmercury in riparian soil, sediments, mine-waste calcines, and moss from abandoned Hg mines in east Guizhou province, southwestern China. Appl Geochem 20:627–638

    CAS  Google Scholar 

  • Rose MT, Patti AF, Little KR, Brown AL, Cavagnaro TR (2014) Chapter two - a meta-analysis and review of plant-growth response to humic substances: practical implications for agriculture. Adv Agron 124:37–89

    CAS  Google Scholar 

  • Senesi N, D'Orazio V, Ricca G (2003) Humic acids in the first generation of EUROSOILS. Geoderma 116:325–344

    CAS  Google Scholar 

  • Serenella N, Diego P, Adele MVA (2002) Physiological effects of humic substances on higher plants. Soil Biol Biochem 34:1527–1536

    Google Scholar 

  • Solerrovira P, Madejón E, Madejón P, CJC P (2010) In situ remediation of metal-contaminated soils with organic amendments: role of humic acids in copper bioavailability. Chemosphere 79:844–849

    CAS  Google Scholar 

  • Stevenson FJ (1982) Humus chemistry: genesis, composition, reactions. Soil Sci 135:129–130

    Google Scholar 

  • Tan K (2014) Humic matter in soil and the environment: principles and controversies, 2nd edn. CRC Press, Boca Raton

    Google Scholar 

  • Tang W, Zeng G, Gong J, Liang J, Xu P, Zhang C, Huang B (2014) Impact of humic/fulvic acid on the removal of heavy metals from aqueous solutions using nanomaterials: a review. Sci Total Environ 468:1014–1027. https://doi.org/10.1016/j.scitotenv.2013.09.044

    Article  CAS  Google Scholar 

  • Thi Anh Thu T, Zhou F, Yang W, Wang M, Quang Toan D, Wang D, Liang D (2018) Detoxification of mercury in soil by selenite and related mechanisms. Ecotoxicol Environ Saf 159:77–84. https://doi.org/10.1016/j.ecoenv.2018.04.029

    Article  CAS  Google Scholar 

  • Ulf S, Andreas D (2010) Competition between disordered iron sulfide and natural organic matter associated thiols for mercury(II)-an EXAFS study. Environ Sci Technol 44:1254–1259

    Google Scholar 

  • Ulf S, Bloom PR, Jin Q, Chung-Min L, Bleam WF (2006) Complexation of mercury(II) in soil organic matter: EXAFS evidence for linear two-coordination with reduced sulfur groups. Environ Sci Technol 40:4174–4180

    Google Scholar 

  • Wang SG, Sun XF, Liu XW, Gong WX (2008) Chitosan hydrogel beads for fulvic acid adsorption: Behaviors and mechanisms. Chem Eng J 142:239–247

    CAS  Google Scholar 

  • Wang Y, Yang R, Zheng J, Shen Z, Xu X (2019) Exogenous foliar application of fulvic acid alleviate cadmium toxicity in lettuce (Lactuca sativa L). Ecotoxicol Environ Saf 167:10–19

    CAS  Google Scholar 

  • Xia K, Skyllberg U, Bleam W, Bloom P, Nater E, Helmke P (1999a) X-ray absorption spectroscopic evidence for the complexation of Hg(II) by reduced sulfur in soil humic substances. Environ Sci Technol 33:257–261

    CAS  Google Scholar 

  • Xia K, Skyllberg UL, Bleam WF, Bloom PR, Nater EA, Helmke PA (1999b) X-ray absorption spectroscopic evidence for the complexation of Hg(II) by reduced sulfur in soil humic substances. Environ Sci Technol 33:257–261

    CAS  Google Scholar 

  • Xing Y, Wang J, Xia J, Liu Z, Zhang Y, Du Y, Wei W (2019) A pilot study on using biochars as sustainable amendments to inhibit rice uptake of Hg from a historically polluted soil in a Karst region of China. Ecotoxicol Environ Saf 170:18–24. https://doi.org/10.1016/j.ecoenv.2018.11.111

    Article  CAS  Google Scholar 

  • Xu J, Bravo AG, Lagerkvist A, Bertilsson S, Sjoblom R, Kumpiene J (2015) Sources and remediation techniques for mercury contaminated soil. Environ Int 74:42–53. https://doi.org/10.1016/j.envint.2014.09.007

    Article  CAS  Google Scholar 

  • Xu Q, Duan DC, Cai QY, Shi JY (2018) Influence of humic acid on Pb uptake and accumulation in tea plants. J Agric Food Chem 66:12327–12334. https://doi.org/10.1021/acs.jafc.8b03556

    Article  CAS  Google Scholar 

  • Xue W et al (2019) Anthropogenic influences on mercury in Chinese soil and sediment revealed by relationships with total organic carbon. Environ Pollut 255:113186. https://doi.org/10.1016/j.envpol.2019.113186

    Article  CAS  Google Scholar 

  • Yang YK, Zhang C, Shi XJ, Lin T, Wang DY (2007) Effect of organic matter and pH on mercury release from soils. J Environ Sci 19:1349–1354. https://doi.org/10.1016/s1001-0742(07)60220-4

    Article  CAS  Google Scholar 

  • Ye W et al (2019) Sustainable management of landfill leachate concentrate through recovering humic substance as liquid fertilizer by loose nanofiltration. Water Res 157:555–563. https://doi.org/10.1016/j.watres.2019.02.060

    Article  CAS  Google Scholar 

  • Yildirim E (2007) Foliar and soil fertilization of humic acid affect productivity and quality of tomato. Acta Agric Scand 57:182–186

    CAS  Google Scholar 

  • Yin R et al (2016) Distribution and geochemical speciation of soil mercury in Wanshan Hg mine: effects of cultivation. Geoderma 272:32–38. https://doi.org/10.1016/j.geoderma.2016.03.003

    Article  CAS  Google Scholar 

  • Yu Y, Wan Y, Camara AY, Li H (2018) Effects of the addition and aging of humic acid-based amendments on the solubility of Cd in soil solution and its accumulation in rice. Chemosphere 196:303–310. https://doi.org/10.1016/j.chemosphere.2018.01.002

    Article  CAS  Google Scholar 

  • Zhang X, Wang Q, Zhang S, Sun X, Zhang Z (2009) Stabilization/solidification (S/S) of mercury-contaminated hazardous wastes using natural zeolite and Portland cement. Acta Sci Circumst 168:1575–1580

    CAS  Google Scholar 

  • Zhang H, Feng X, Larssen T, Shang L, Li P (2010) Bioaccumulation of methylmercury versus Inorganic mercury in rice (Oryza sativa L) grain. Environ Sci Technol 44:4499–4504. https://doi.org/10.1021/es903565t

    Article  CAS  Google Scholar 

  • Zhang J, Li H, Zhou Y, Dou L, Cai L, Mo L, You J (2018) Bioavailability and soil-to-crop transfer of heavy metals in farmland soils: a case study in the Pearl River Delta, South China. Environmental Pollution 235:710–719

    CAS  Google Scholar 

  • Zhu H (2015) Effects of rice residue incorporation on the speciation and exposure risk of mercury in mining paddy soils Nanjing University

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Funding

This work was supported by the Institute for Scientific Research of Karst Area of NSFC-GZGOV (U1612442) and the Ministry of Science and Technology of China for the State Key Research and Development Project (2016YFC0400702).

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Authors and Affiliations

  1. College of Environmental Science and Energy, South China University of Technology, Guangzhou, 510006, China

    Yue Liu, Liangliang Zhi & Shaoqi Zhou

  2. Guizhou Academy of Sciences, Guiyang, 550001, China

    Shaoqi Zhou

  3. Key Laboratory of Subtropical Building Sciences, South China University of Technology, Guangzhou, 510641, China

    Shaoqi Zhou

  4. Key Laboratory of Environmental Protection and Eco-Remediation of Guangdong Regular Higher Education Institutions, South China University of Technology, Guangzhou, 510006, China

    Shaoqi Zhou

  5. Guizhou Academy of Testing and Analysis, Guiyang, 550001, China

    Feng Xie

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  1. Yue Liu
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Liu, Y., Zhi, L., Zhou, S. et al. Effects of mercury binding by humic acid and humic acid resistance on mercury stress in rice plants under high Hg/humic acid concentration ratios. Environ Sci Pollut Res 27, 18650–18660 (2020). https://doi.org/10.1007/s11356-020-08328-9

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