Revealing the active period and type of tetracycline stress on Chinese cabbage (Brassica rapa L.) during seed germination and post-germination

Abstract

Stresses of antibiotics can cause strains (i.e. effects) on seed at germination and post-germination stages. But there is a lack of research on the period and type of the effects at present. In this study, Chinese cabbage (Brassica rapa L.), a commonly used crop, was selected to investigate the effect of tetracycline (TC), a major-use antibiotic, on its seed during different periods of the stages. Results showed that there were no significant differences among the germination energy (GE) of control (CK) and treatments, but radicle length (RL) of the treatments, the exposure to TC at post-germination stage (i.e. radicle elongation stage), was all significantly less than that of CK. The initial stage of radicle elongation was the earliest and most sensitive period at which the stress of TC caused the plastic effect on seed. Moreover, the action of TC stress on seed did not have a delayed characteristic. The result of RL was identical to the leakage of intracellular substances at radicle fast elongation stage, but not the Evan’s blue trapped by radicle. We concluded that TC inhibited the elongation of radicle through weakening the cellular metabolic activity rather than leading to the loss of cellular membrane integrity. It should be paid more attention to the phytotoxicity of TC in the field due to its active characteristics revealed in our study.