Determination of acute oral toxicity of glyphosate isopropylamine salt in rats

Abstract

In this study, we aim to determine the median lethal dose (LD₅₀) of glyphosate isopropylamine salt (GI), which is commonly used in the world and especially in Turkey against to weeds, in male and female rats by using the probit or logit analysis method. A total of 140 Wistar rats were used, including 70 females and 70 males. To determine LD₅₀, the male and female rats were randomized into 7 groups made up of 10 animals in each group. At doses of 6000, 6500, 7000, 7500, 8000, 8500, and 9000 mg / kg, GI was administered to the male and female rats by oral gavage. After dosing, the animals were periodically monitored for 14 days. No deaths were observed after 48 h of herbicide application. In this study, only logit analysis was used for the LD₅₀ value to be calculated in the male rats within 24 h, while other analyses were carried out with the probit method. In the female and male rats, the LD₅₀ levels of GI between 24 and 48 h were determined as 7444.26–7878.50 mg/kg and 7203.58–7397.25 mg/kg, respectively. According to these results, it was concluded that female rats are more sensitive to GI than male rats. We believe that the findings that were obtained will guide researchers, clinicians, and toxicologists through preventive and curative studies against acute poisoning that may occur with GI.