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Detoxification and reproductive system-related gene expression following exposure to Cu(OH)2 nanopesticide in water flea (Daphnia magna Straus 1820)

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Abstract

The extensive use of copper-based nanopesticides in agriculture has led to their release into the aquatic environment and causes a potential risk to aquatic biota. However, there is a lack of knowledge regarding the possible toxic effect of these nanopesticides on non-target aquatic organisms including invertebrates. Therefore, in this study, effects of commonly used copper-based nanopesticide “Kocide 3000” on gene expression related to detoxification (cyp360a8, gst, P-gp, and hr96) and reproductive system (cut, cyp314, dmrt93, and vtg) in Daphnia magna was investigated through an acute toxicity test. In general, exposure to the nanopesticide caused significant down-regulation of detoxification genes after 24 h and then significant up-regulation after 48 h. Exposure to the nanopesticide, however, significantly induced cut expression after 24 h. Moreover, dmrt93 and vtg genes were up-regulated after 48 h exposure to the nanopesticide. On the other hand, the expression of dmrt93 and vtg down-regulated at high concentration of Cu(OH)2 nanopesticide (1.5 ppm) after 96 h. The results of this study provide first evidence into the crucial role of genes related to detoxification and reproductive system in response to Cu(OH)2 nanopesticide. The use of physiological, biochemical bioassays, as well as gene expression, can help explain the toxic effect of copper-based nanopesticides and provide more insight into the exact mechanism of toxicity in non-target aquatic organisms.

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Authors and Affiliations

  1. Department of Agricultural Biotechnology, Faculty of Agriculture, Ataturk University, 25240, Erzurum, Turkey

    Feyza Icoglu Aksakal

  2. Department of Basic Sciences, Faculty of Fisheries, Ataturk University, 25240, Erzurum, Turkey

    Harun Arslan

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  1. Feyza Icoglu Aksakal
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  2. Harun Arslan
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Correspondence to Feyza Icoglu Aksakal.

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Aksakal, F.I., Arslan, H. Detoxification and reproductive system-related gene expression following exposure to Cu(OH)2 nanopesticide in water flea (Daphnia magna Straus 1820). Environ Sci Pollut Res 27, 6103–6111 (2020). https://doi.org/10.1007/s11356-019-07414-x

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