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Physiological response of Secale cereale L. seedlings under freezing-thawing and alkaline salt stress

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Abstract

Freezing-thawing and saline-alkaline are the major abiotic stress for the pasture in most high-latitude areas, which are serious threats to the yield of pasture. In this study, the osmotic adjustment substances, membrane lipid peroxidation, and antioxidant enzymes activities of rye (Secale cereale L., cv. Dongmu-70) seedlings under different treatments: CK (no treatment), SC (Na2CO3 treatment), FT (freezing-thawing treatment), and FT+SC (combined Na2CO3 and freezing-thawing treatments), were investigated. At the freezing stage, the content of MDA and proline, the activity of APX, SOD, and POD increased with the decrease of the temperature in the leaves of rye seedlings in FT and FT+SC treatments and reached the maximum value at − 5 °C. In addition, the content of protein and H2O2, CAT activity reached the maximum value at 0 °C; the damage is larger under low temperature stress at 0 °C and − 5 °C in rye seedling. At the thawing stage, the content of MDA and H2O2 in seedling leaves decreased in FT and FT + SC treatments. These results demonstrated that proline content and antioxidant enzymes activities could play an important role in protecting cytomembrane and scavenging ROS respectively in rye under alkaline salt stress and freezing-thawing stress. The result also indicated rye seedlings were subjected to a freezing-thawing stress which resulted in a reversible (recoverable) injury.

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Funding

This work was sponsored by the National Natural Science Foundation of China (Grant No. 31772669).

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Authors and Affiliations

  1. Key Laboratory of Groundwater Resources and Environment of the Ministry of Education, College of New Energy and Environment, Jilin University, Changchun, 130012, Jilin Province, China

    Ze Gong, Weiwei Chen, Guozhang Bao & Jiaxing Sun

  2. College of Animal Science, Jilin University, Changchun, 130012, Jilin Province, China

    Xuemei Ding

  3. The Administration of Jingyu Water Conservation, Jingyu, 135200, Jilin Province, China

    Cunxin Fan

Authors
  1. Ze Gong
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  2. Weiwei Chen
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  3. Guozhang Bao
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  4. Jiaxing Sun
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  5. Xuemei Ding
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  6. Cunxin Fan
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Correspondence to Guozhang Bao.

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Responsible editor: Philippe Garrigues

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Gong, Z., Chen, W., Bao, G. et al. Physiological response of Secale cereale L. seedlings under freezing-thawing and alkaline salt stress. Environ Sci Pollut Res 27, 1499–1507 (2020). https://doi.org/10.1007/s11356-019-06799-z

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  • DOI: https://doi.org/10.1007/s11356-019-06799-z

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