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Characterization and chromium biosorption potential of extruded polymeric substances from Synechococcus mundulus induced by acute dose of gamma irradiation

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Abstract

This study characterized the extruded polymeric substances (EPS) secreted from Synechococcus mundulus cultures under the effect of 2-KGy gamma irradiation dose. The EPS demonstrated seven monosaccharides, two uronic acids and several chemical functional groups: O–H, N–H, =C–H, C=C, C=O, COO–, O–SO3, C–O–C and a newly formed peak at 1593 cm−1 (secondary imide). The roughness of EPS was 96.71 nm and only 28.4% total loss in weight was observed at 800 °C with a high degree of crystallinity quantified as CIDSC (0.722) and CIXRD (0.718). Preliminary comparative analyses of EPS exhibited high protein content in the radiologically modified (R-EPS) than control (C-EPS). Modified EPS were characterized with a high biosorption efficiency, which could be attributed to its high content of uronic acids, protein and sulphates as well as various saccharide monomers. Data revealed that 0.0213 mg L−1 h−1 is the maximum biosorption rate (SBRmax) of Cr(VI) for R-EPS, whereas 0.0204 mg L−1 h−1 SBRmax for the C-EPS respectively.

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References

  • Abbas T, Rizwan M, Ali S, Adrees M, Mahmood A, Rehman MZ, Ibrahim M, Arshad M, Qayyum MF (2018) Biochar application increased the growth and yield and reduced cadmium in drought stressed wheat grown in an aged contaminated soil. Ecotoxicol Environ Saf 148:825–833

    CAS  Google Scholar 

  • Ali S, Rizwan M, Noureen S, Anwar S, Ali B, Naveed M, Abd_Allah E, Alqarawi AA, Ahmad P (2019) Combined use of biochar and zinc oxide nanoparticle foliar spray improved the plant growth and decreased the cadmium accumulation in rice (Oryza sativa L.) plant. Environ Sci Pollut Res 26:11288–11299

    CAS  Google Scholar 

  • Andersen RA (2005) Algal culturing techniques. Elsevier, Amsterdam p589

    Google Scholar 

  • Association APH, Association AWW (1989) Standard methods for the examination of water and wastewater. American Public Health Association, Washington, D.C.

    Google Scholar 

  • Bhunia B, Uday USP, Oinam G, Mondal A, Bandyopadhyay TK, Tiwari ON (2018) Characterization, genetic regulation and production of cyanobacterial exopolysaccharides and its applicability for heavy metal removal. Carbohydr Polym 179:228–243

    CAS  Google Scholar 

  • Billi D, Friedmann EI, Hofer KG, Caiola MG, Ocampo-Friedmann R (2000) Ionizing-radiation resistance in the desiccation-tolerant cyanobacterium Chroococcidiopsis. Appl Environ Microbiol 66:1489–1492

    CAS  Google Scholar 

  • Bryan R, Zaragoza O, Zhang T, Ortiz G, Casadevall A, Dadachova E (2005) Radiological studies reveal radial differences in the architecture of the polysaccharide capsule of Cryptococcus neoformans. Eukaryot Cell 4:465–475

    CAS  Google Scholar 

  • Chen JP, Lin YS (2007) Decolorization of azo dye by immobilized Pseudomonas luteola entrapped in alginate–silicate sol–gel beads. Process Biochem 42:934–942

    CAS  Google Scholar 

  • Chen M, Wu WW, Nanz D, Sticher O, Leonticins DH (1997) Five triterpene saponins from Leontice kiangnanensis. Phytochem 44:497–504

    CAS  Google Scholar 

  • Chowdhury S, Manna S, Saha P, Basak R, Sen R, Roy D, Adhikari B (2011) Composition analysis and material characterization of an emulsifying extracellular polysaccharide (EPS) produced by Bacillus megaterium RB-05: a hydrodynamic sediment-attached isolate of freshwater origin. J Appl Microbiol 111:1381–1393

    CAS  Google Scholar 

  • De Philippis R, Vincenzini M (1998) Exocellular polysaccharides from cyanobacteria and their possible applications. FEMS Microbiol Rev 22:151–175

    Google Scholar 

  • Dean JA (1995) Analytical chemistry handbook. McGraw-Hill Companies, New York City

    Google Scholar 

  • Dobrowolski R, Szcześ A, Czemierska M, Jarosz-Wikołazka A (2017) Studies of cadmium (II), lead (II), nickel (II), cobalt (II) and chromium (VI) sorption on extracellular polymeric substances produced by Rhodococcus opacus and Rhodococcus rhodochrous. Bioresour Technol 225:113–120

    CAS  Google Scholar 

  • Dubois M, Gilles KA, Hamilton JK, Rebers PT, Smith F (1956) Colorimetric method for determination of sugars and related substances. Anal Chem 28:350–356

    CAS  Google Scholar 

  • Freitas F, Alves VD, Pais J, Costa N, Oliveira C, Mafra L, Hilliou L, Oliveira R, Reis MA (2009) Characterization of an extracellular polysaccharide produced by a Pseudomonas strain grown on glycerol. Bioresour Technol 100:859–865

    CAS  Google Scholar 

  • Gómez-Ordóñez E, Rupérez P (2011) FTIR-ATR spectroscopy as a tool for polysaccharide identification in edible brown and red seaweeds. Food Hydrocoll 25:1514–1520

    Google Scholar 

  • Gupta P, Diwan B (2017) Bacterial exopolysaccharide mediated heavy metal removal: a review on biosynthesis mechanism and remediation strategies. Biotechnol Rep 13:58–71

    Google Scholar 

  • Han P, Guo RJ, Shen SG, Yan R, Wu YK, Yao SY, Wang HY, Jia SR (2018) Proteomic profiling of Nostoc flagelliforme reveals the common mechanism in promoting polysaccharide production by different light qualities. Biochem Eng J 132:68–78

    CAS  Google Scholar 

  • Hussain A, Ali S, Rizwan M, Rehman MZ, Javed MR, Imran M, Chatha SA, Nazir R (2018) Zinc oxide nanoparticles alter the wheat physiological response and reduce the cadmium uptake by plants. Environ Pollut 242:1518–1526

    CAS  Google Scholar 

  • Hussein M, Abou-ElWafa GS, Shaaban-Dessuuki SA, Hassan NI (2015) Characterization and antioxidant activity of exopolysaccharide secreted by Nostoc carneum. Int J Pharm 11:432–439

    CAS  Google Scholar 

  • Insulkar P, Kerkar S, Lele S (2018) Purification and structural-functional characterization of an exopolysaccharide from Bacillus licheniformis PASS26 with in-vitro antitumor and wound healing activities. Int J Biol Macromol 120:1441–1450

    CAS  Google Scholar 

  • Jodłowska S, Śliwińska S (2014) Effects of light intensity and temperature on the photosynthetic irradiance response curves and chlorophyll fluorescence in three picocyanobacterial strains of Synechococcus. Photosynthetica 52:223–232

    Google Scholar 

  • Kavita K, Mishra A, Jha B (2011) Isolation and physico-chemical characterisation of extracellular polymeric substances produced by the marine bacterium Vibrio parahaemolyticus. Biofouling 27:309–317

    CAS  Google Scholar 

  • Khalil U, Shakoor MB, Ali S, Rizwan M (2018) Tea waste as a potential biowaste for removal of hexavalent chromium from wastewater: equilibrium and kinetic studies. Arab J Geosci 11:1–11

    Google Scholar 

  • Khanna Y, Kuhn W (1997) Measurement of crystalline index in nylons by DSC: complexities and recommendations. J Polym Sci B Polym Phys 35:2219–2231

    CAS  Google Scholar 

  • Korotaeva N, Ivanova M, Suvorova G, Borovskii G (2018) The impact of the environmental factors on the photosynthetic activity of common pine (Pinus sylvestris) in spring and in autumn in the region of Eastern Siberia. J For Res 29:1465–1473

    CAS  Google Scholar 

  • Kumar CG, Joo HS, Choi JW, Koo YM, Chang CS (2004) Purification and characterization of an extracellular polysaccharide from haloalkalophilic Bacillus sp. I-450. Enzym Microb Technol 34:673–681

    CAS  Google Scholar 

  • Lan G, Hong X, Fan Q, Luo B, Shi P, Chen X (2014) Removal of hexavalent chromium in wastewater by polyacrylamide modified iron oxide nanoparticle. J Appl Polym Sci 131:40945–40955

    Google Scholar 

  • Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurement with the Folin phenol reagent. J Biol Chem 193:265–275

    CAS  Google Scholar 

  • Magdoub MIN, EL-Fouly M, Meleigy SA, Aita OA, Hendawy WS (2010) Enhancement productivity of lactic acid bacteria (LAB) to produced ex polysaccharide. 2nd International conference on radiation sciences and applications 28:3

  • Maurice PA, Pullin MJ, Cabaniss SE, Zhou Q, Namjesnik-Dejanovic K, Aiken GR (2002) A comparison of surface water natural organic matter in raw filtered water samples, XAD, and reverse osmosis isolates. Water Res 36:2357–2371

    CAS  Google Scholar 

  • Mishra A, Kavita K, Jha B (2011) Characterization of extracellular polymeric substances produced by microalgae Dunaliella salina. Carbohydr Polym 83:852–857

    CAS  Google Scholar 

  • Mota R, Guimarães R, Büttel Z, Rossi F, Colica G, Silva CJ et al (2013a) Production and characterization of extracellular carbohydrate polymer from Cyanothece sp. CCY 0110. Carbohydr Polym 92(2):1408–1415

    CAS  Google Scholar 

  • Netzahuatl-Muñoz AR, del Carmen Cristiani-Urbina M, Cristiani-Urbina E (2015) Chromium biosorption from Cr (VI) aqueous solutions by Cupressus lusitanica bark: kinetics, equilibrium and thermodynamic studies. PLoS One 10(9):e0137086

    Google Scholar 

  • Ozturk S, Aslim B (2010) Modification of exopolysaccharide composition and production by three cyanobacterial isolates under salt stress. Environ Sci Pollut Res 17:595–602

    CAS  Google Scholar 

  • Ozturk S, Aslim B, Suludere Z (2009) Evaluation of chromium (VI) removal behaviour by two isolates of Synechocystis sp. in terms of exopolysaccharide (EPS) production and monomer composition. Bioresour Technol 100:5588–5559

    CAS  Google Scholar 

  • Ozturk S, Aslim B, Suludere Z, Tan S (2014) Metal removal of cyanobacterial exopolysaccharides by uronic acid content and monosaccharide composition. Carbohydr Polym 101:265–271

    CAS  Google Scholar 

  • Padmavathi AR, Periyasamy M, Pandian SK (2015) Assessment of 2, 4-di-tert-butylphenol induced modifications in extracellular polymeric substances of Serratia marcescens. Bioresour Technol 188:185–189

    CAS  Google Scholar 

  • Parikh A, Madamwar D (2006) Partial characterization of extracellular polysaccharides from cyanobacteria. Bioresour Technol 97(15):1822–1827

    CAS  Google Scholar 

  • Pereira Duta F, Pessôa de França F, de Almeida Lopes LM (2006) Optimization of culture conditions for exopolysaccharides production in Rhizobium sp. using the response surface method. Electron J Biotechnol 9

    Google Scholar 

  • Pereira L, Sousa A, Coelho H, Amado AM, Ribeiro-Claro PJ (2003) Use of FTIR, FT-Raman and 13C-NMR spectroscopy for identification of some seaweed phycocolloids. Biomol Eng 20:223–228

    CAS  Google Scholar 

  • Pereira S, Zille A, Micheletti E, Moradas-Ferreira P, De Philippis R, Tamagnini P (2009) Complexity of cyanobacterial exopolysaccharides: composition, structures, inducing factors and putative genes involved in their biosynthesis and assembly. FEMS Microbiol Rev 33:917–941

    CAS  Google Scholar 

  • Rani RP, Anandharaj M, Ravindran AD (2018) Characterization of a novel exopolysaccharide produced by Lactobacillus gasseri FR4 and demonstration of its in vitro biological properties. Int J Biol Macromol 109:772–783

    CAS  Google Scholar 

  • Reddy K, Soper B, Tang J, Bradley R (1996) Phenotypic variation in exopolysaccharide production in the marine, aerobic nitrogen-fixing unicellular cyanobacterium Cyanothece sp. World J Microbiol Biotechnol 12:311–318

    CAS  Google Scholar 

  • Ricou P, Pinel E, Juhasz N (2005) Temperature experiments for improved accuracy in the calculation of polyamide-11 crystallinity by X-ray diffraction, Advances in X-ray Analysis, vol 48. International Centre for Diffraction Data, Newtown Square, pp 170–175

    Google Scholar 

  • Richert L, Golubic S, Le Guédès R, Ratiskol J, Payri C, Guezennec J (2005) Characterization of exopolysaccharides produced by cyanobacteria isolated from Polynesian microbial mats. Curr Microbiol 51:379–384

    CAS  Google Scholar 

  • Rizwan M, Ali S, Hussain A, Ali Q, Shakoor MB, Rehman MZ, Farid M, Asma M (2017) Effect of zinc-lysine on growth, yield and cadmium uptake in wheat (Triticum aestivum L.) and health risk assessment. Chemosphere 187:35–42

    CAS  Google Scholar 

  • Rizwan M, Ali S, Rehman MZ, Rinklebe J, Tsang DC, Bashir A, Maqbool A, Tack FM, Ok YS (2018) Cadmium phytoremediation potential of Brassica crop species: a review. Sci Total Environ 631:1175–1191

    Google Scholar 

  • Ruber J, Bauer FR, Millard AD, Raeder U, Geist J, Zwirglmaier K (2016) Synechococcus diversity along a trophic gradient in the Osterseen Lake District, Bavaria. Microbiol 162:2053–2063

    CAS  Google Scholar 

  • Ruffing A, Jensen TJ, Strickland LM (2016) Ionizing radiation resistance in cyanobacteria (No. SAND2016-4406C). Sandia National Lab.(SNLNM), Albuquerque, NM (United States)

  • Sahmoune MN, Louhab K, Boukhiar A (2011) Advanced biosorbents materials for removal of chromium from water and wastewaters. Environ Prog Sustain Energy 30:284–293

    CAS  Google Scholar 

  • Saravanan C, Shetty PKH (2016) Isolation and characterization of exopolysaccharide from Leuconostoc lactis KC117496 isolated from idli batter. Int J Biol Macromol 90:100–106

    CAS  Google Scholar 

  • Sardar UR, Bhargavi E, Devi I, Bhunia B, Tiwari ON (2018) Advances in exopolysaccharides based bioremediation of heavy metals in soil and water: a critical review. Carbohydr Polym 199:353–364

    Google Scholar 

  • Sardari RR, Kulcinskaja E, Ron EY, Björnsdóttir S, Friðjónsson ÓH, Hreggviðsson GÓ, Karlsson EN (2017) Evaluation of the production of exopolysaccharides by two strains of the thermophilic bacterium Rhodothermus marinus. Carbohydr Polym 156:1–8

    CAS  Google Scholar 

  • Sasaki H, Shirato S, Tahara T, Sato K, Takenaka H (2013) Accumulation of radioactive cesium released from Fukushima Daiichi Nuclear Power Plant in terrestrial cyanobacteria Nostoc commune. Microbes Environ 28:466–469

    Google Scholar 

  • Sattar MS, Shakoor MB, Ali S, Rizwan M, Niazi NK, Jilani A (2019) Comparative efficiency of peanut shell and peanut shell biochar for removal of arsenic from water. Environ Sci Pollut Res 26:18624–18635. https://doi.org/10.1007/s11356-019-05185-z

    Article  CAS  Google Scholar 

  • Schopf JW (2000) The fossil record: tracing the roots of the cyanobacterial lineage. In: The ecology of cyanobacteria. Springer, Berlin, pp 13–35

    Google Scholar 

  • Shen L, Li Z, Wang J, Liu A, Li Z, Yu R, Wu X, Liu Y, Li J, Zeng W (2018) Characterization of extracellular polysaccharide/protein contents during the adsorption of Cd (II) by Synechocystis sp. PCC6803. Environ Sci Pollut Res:1–10

  • Shevchenko T (1999) Accumulation of radionuclides by communities of Periphytic algae in the cooling pond of the Chernobyl NPP (8 years after the accident). Hydrobiol J 35(2):117–130

    Google Scholar 

  • Shokier H, EI-Adly A, Hussein H, Shabon M, EI-Shanshoury I (2010) Effect of gamma rays on antibiotic resistance of Staphylococcus aureus and Pseudomonas aeruginosa isolated from human skin. J Radiat Res Appl Sci 3:1687–1507

    Google Scholar 

  • Shuhong Y, Meiping Z, Hong Y, Han W, Shan X, Yan L, Jihui W (2014) Biosorption of Cu2+, Pb2+ and Cr6+ by a novel exopolysaccharide from Arthrobacter ps-5. Carbohydr Polym 101:50–56

    Google Scholar 

  • Shuryak I (2019) Review of microbial resistance to chronic ionizing radiation exposure under environmental conditions. J Environ Radioact 196:50–63

    CAS  Google Scholar 

  • Singh OV, Gabani P (2011) Extremophiles: radiation resistance microbial reserves and therapeutic implications. J Appl Microbiol 110:851–861

    CAS  Google Scholar 

  • Singh H, Fernandes T, Apte SK (2010) Unusual radioresistance of nitrogen-fixing cultures of Anabaena strains. J Biosci 35:427–434

    Google Scholar 

  • Singh RP, Shukla MK, Mishra A, Kumari P, Reddy C, Jha B (2011) Isolation and characterization of exopolysaccharides from seaweed associated bacteria Bacillus licheniformis. Carbohydr Polym 84:1019–1026

    CAS  Google Scholar 

  • Sinha RP, Häder DP (2006) UV-protectants in cyanobacteria. Plant Sci 174(2008):278–289

    Google Scholar 

  • Skuja H (1964) Grundzüge der Algenflora und Algenvegetation der Fjeldgegenden um Abisko in Schwedish-Lappland. Nova Acta Regiae Societas Scientarum Upsaliensis 18:465

    Google Scholar 

  • Stanier R, Kunisawa R, Mandel M, Cohen-Bazire G (1971) Purification and properties of unicellular blue-green algae (order Chroococcales). Bacteriol Rev 35:171

    CAS  Google Scholar 

  • Stuart BH (2004) Infrared spectroscopy: fundamentals and applications. Wiley, Hoboken

    Google Scholar 

  • Su X, Wang Y, Xue B, Hashmi MZ, Lin H, Chen J, Wang Z, Mei R, Sun F (2019) Impact of resuscitation promoting factor (Rpf) in membrane bioreactor treating high-saline phenolic wastewater: performance robustness and Rpf-responsive bacterial populations. Chem Eng J 357:715–723

    CAS  Google Scholar 

  • Tahir U, Yasmin A (2019) Role of bacterial extracellular polymeric substances (EPS) in uptake and accumulation of co-contaminants. Int J Environ Sci Technol:1–12

  • Trabelsi L, M’sakni NH, Ouada HB, Bacha H, Roudesli S (2009) Partial characterization of extracellular polysaccharides produced by cyanobacterium Arthrospira platensis. Biotechnol Bioprocess Eng 14:27–31

    CAS  Google Scholar 

  • Wang K, Li W, Rui X, Chen X, Jiang M, Dong M (2014) Characterization of a novel exopolysaccharide with antitumor activity from Lactobacillus plantarum 70810. Int J Biol Macromol 63:133–139

    CAS  Google Scholar 

  • Wei L, Li Y, Noguera DR, Zhao N, Song Y, Ding J, Zhao Q, Cui F (2017) Adsorption of Cu2+ and Zn2+ by extracellular polymeric substances (EPS) in different sludges: Effect of EPS fractional polarity on binding mechanism. J Hazard Mater 321:473–483

    CAS  Google Scholar 

  • Whitton BA (2012) Ecology of cyanobacteria II: their diversity in space and time. Springer Science & Business Media, Berlin

    Google Scholar 

  • Yun U, Park H (2003) Physical properties of an extracellular polysaccharide produced by Bacillus sp. CP912. Lett Appl Microbiol 36:282–287

    Google Scholar 

  • Zeng J, Gao JM, Chen YP, Yan P, Dong Y, Shen Y et al (2016) Composition and aggregation of extracellular polymeric substances (EPS) in hyperhaline and municipal wastewater treatment plants. Sci Rep 6:1–14

    Google Scholar 

  • Zhang H, Tang Y, Cai D, Liu X, Wang X, Huang Q, Yu Z (2010) Hexavalent chromium removal from aqueous solution by algal bloom residue derived activated carbon: equilibrium and kinetic studies. J Hazard Mater 181(1-3):801–808

    CAS  Google Scholar 

  • Zhang J, Cao Y, Wang J, Guo X, Zheng Y, Zhao W, Mei X, Guo T, Yang Z (2016) Physicochemical characteristics and bioactivities of the exopolysaccharide and its sulphated polymer from Streptococcus thermophilus GST-6. Carbohydr Polym 146:368–375

    CAS  Google Scholar 

  • Zhao M, Yang N, Yang B, Jiang Y, Zhang G (2007) Structural characterization of water-soluble polysaccharides from Opuntia monacantha cladodes in relation to their anti-glycated activities. Food Chem 105:1480–1486

    CAS  Google Scholar 

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This study was supported by the Genetic Engineering and Biotechnology Research Institute, University of Sadat City, Sadat City, Egypt

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Authors and Affiliations

  1. Department of Botany, Faculty of Science, Mansoura University, Mansoura, Egypt

    Mervat H. Hussein & Muhammad A. Abuelmagd

  2. Department of Biology, Faculty of Sciences and Arts Khulais, University of Jeddah, Jeddah, Saudi Arabia

    Ragaa A. Hamouda

  3. Department of Microbial Biotechnology, Genetic Engineering and Biotechnology Research Institute, University of Sadat City, Sadat City, Egypt

    Ragaa A. Hamouda

  4. Biological Application Department, Nuclear Research Center, Atomic Energy Authority, Cairo, Egypt

    Abdel Monsef A. Elhadary

  5. Department of Environmental Sciences and Engineering, Government College University, Faisalabad, 38000, Pakistan

    Shafaqat Ali & Muhammad Rizwan

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  1. Mervat H. Hussein
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Hussein, M.H., Hamouda, R.A., Elhadary, A.M.A. et al. Characterization and chromium biosorption potential of extruded polymeric substances from Synechococcus mundulus induced by acute dose of gamma irradiation. Environ Sci Pollut Res 26, 31998–32012 (2019). https://doi.org/10.1007/s11356-019-06202-x

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