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Investigating the effect of methyl jasmonate and melatonin on resistance of Malus crabapple ‘Hong Jiu’ to ozone stress

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Abstract

Ozone (O3) is an adverse environmental factor posing damage to ornamental plants. Thus, it is important to seek an effective way of enhancing plant tolerance to O3-induced damage. Methyl jasmonate (MJ) and melatonin (MT) are plant growth regulators (PGRs) involved in plant abiotic stress responses. In this study, compared with the control group of plants without ozone, the influence of exogenous MJ (0, 10, 50, 100, and 150 μM) and MT (0, 0.1, 0.5, 2.5, and 12.5 μM) on the resistance of Malus crabapple ‘Hong Jiu’ was evaluated under O3 stress (100 ± 10 nL/L for 3 h). Our data revealed that levels of MDA were significantly enhanced following O3 treatment compared with plants without O3. O3 induced the activities of antioxidant enzymes and the accumulation of non-enzymatic antioxidants. While lower malondialdehyde (MDA) content, greater activities of antioxidant enzymes, and higher levels of soluble protein and non-enzymatic antioxidants were observed in PGRs-pretreated plants than in non-PGRs-pretreated plants under O3 stress. Based on the above results and air pollution tolerance index (APTI), an exogenous supply of MJ and MT to Malus crabapple ‘Hong Jiu’ seedlings was protective for O3-induced toxicity. The present study provides new insights into the mechanisms of MJ and MT amelioration of O3-induced oxidative stress damages in Malus crabapple ‘Hong Jiu.’

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Abbreviations

ANOVA:

Analysis of variance

APTI:

Air pollution tolerance index

AsA:

Ascorbic acid

CAT:

Catalase

GSH:

Glutathione

H2O2 :

Hydrogen peroxide

MDA:

Malondialdehyde

MJ:

Methyl jasmonate

MT:

Melatonin

NBT:

Nitroblue tetrazolium

O2 :

Oxygen

O2 − :

Superoxide anion

O3 :

Ozone

OTC:

Open-top chamber

PGRs:

Plant growth regulators

POD:

Peroxidase

ROS:

Reactive oxygen species

RWC:

Relative water content

SD:

Standard deviation

SDAU:

Shandong Agricultural University

SE:

Standard error

SOD:

Superoxide dismutase

TBA:

2-Thiobarbituric acid

TCH:

Total chlorophyll

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Funding

The work was supported by grants from the National Natural Science Foundation of China (CN) (31730080) and the National Key Research and Development Project (2016YFC0501505).

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Authors and Affiliations

  1. College of Forestry, Shandong Agricultural University, Tai-An, Shandong, China

    Yanfen Qiu, Kai An, Jingjing Sun, Xiaojun Gong, Li Ma, Shuqing Wu & Yanling Wang

  2. State Key Laboratory of Crop Biology, College of Horticulture Science and Engineering, Shandong Agricultural University, Tai-An, Shandong, China

    Xuesen Chen, Shenghui Jiang & Zongying Zhang

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  1. Yanfen Qiu
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  2. Kai An
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  3. Jingjing Sun
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  10. Yanling Wang
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Correspondence to Yanling Wang.

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Qiu, Y., An, K., Sun, J. et al. Investigating the effect of methyl jasmonate and melatonin on resistance of Malus crabapple ‘Hong Jiu’ to ozone stress. Environ Sci Pollut Res 26, 27761–27768 (2019). https://doi.org/10.1007/s11356-019-05946-w

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