Degradation of Nystatin in aqueous medium by coupling UV-C irradiation, H2O2 photolysis, and photo-Fenton processes

  • Amira Boucenna
  • Nihal Oturan
  • Malika ChabaniEmail author
  • Souad Bouafia-Chergui
  • Mehmet A. OturanEmail author
Research Article


Oxidative degradation and mineralization of the antifungal drug Nystatin (NYS) was investigated using photochemical advanced oxidation processes UV-C irradiation (280–100 nm), H2O2 photolysis (UV/H2O2), and photo-Fenton (UV/H2O2/Fe3+). The effect of operating parameters such as [H2O2], [Fe3+], and [NYS] initial concentrations on degradation efficiency and mineralization ability of different processes was comparatively examined in order to optimize the processes. Photo-Fenton was found to be the most efficient process attaining complete degradation of 0.02 mM (19.2 mg L−1) NYS at 2 min and a quasi-complete mineralization (97%) of its solution at 5 h treatment while UV/H2O2 and UV-C systems require significantly more time for complete degradation and lower mineralization degrees. The degradation and mineralization kinetics were affected by H2O2 and Fe3+ initial concentration, the optimum dosages being 4 mM and 0.4 mM, respectively. Consumption of H2O2 during photo-Fenton treatment is very fast during the first 30 min leading to the appearance of two stages in the mineralization. The evolution of toxicity of treated solutions was assessed and confirmed the effectiveness of photo-Fenton process for the detoxification of NYS solution at the end of treatment. Application to real wastewater from pharmaceutical industry containing the target molecule NYS showed the effectiveness of photo-Fenton process since it achieved 92% TOC removal rate at 6-h treatment time.


Advanced oxidation processes Photo-Fenton Mineralization Toxicity Water treatment UV-C irradiations UV/H2O2 



Amira Boucenna is grateful to the University of Sciences and Technology Houari Boumediene, Algeria, for the financial support and to Laboratoire de Géomatériaux et Environnement, University Paris-Est, France, for the technical support.


  1. Ahmed MM, Brienza M, Goetz V, Chiron S (2014) Solar photo-Fenton using peroxymonosulfate for organic micropollutants removal from domestic wastewater: comparison with heterogeneous TiO2 photocatalysis. Chemosphere 117:256–261CrossRefGoogle Scholar
  2. Ambuludi LS, Panizza M, Oturan N, Oturan MA (2014) Removal of the anti-inflammatory drug ibuprofen from water using homogeneous photocatalysis. Catal Today 224:29–33CrossRefGoogle Scholar
  3. Andreozzi R, Caprio V, Insola A, Marotta R (1999) Advanced oxidation processes (AOP) for water purification and recovery. Catal Today 53:51–59CrossRefGoogle Scholar
  4. Andreozzi R, Caprio V, Ciniglia C, De Champdoré M, Lo Giudici R, Marota R, Zuccato E (2004) Antibiotics in the environment: occurrence in Italian STPs, fate, and preliminary assessment on algal toxicity of amoxicillin. Environ Sci Technol 38:6832–6838CrossRefGoogle Scholar
  5. Augugliaro V, Litter M, Palmisano L, Soria J (2006) The combination of heterogeneous photocatalysis with chemical and physical operations: a tool for improving the photoprocess performance. J Photochem Photobiol 7:127–144CrossRefGoogle Scholar
  6. Bartels P, Von Tűmpling W (2007) Solar radiation influence on the decomposition process of diclofenac in surface waters. Sci Total Environ 374:143–155CrossRefGoogle Scholar
  7. Benitez FJ, Acero JL, Real FJ et al (2011) Comparison of different chemical oxidation treatments for the removal of selected pharmaceuticals in water matrices. Chem Eng J 168:1149–1156Google Scholar
  8. Bocos E, Oturan N, Pazos M, Sanromán MA, Oturan MA (2016) Elimination of radiocontrast acid agent diatrizoic by photo-Fenton process and enhanced treatment by coupling with electro-Fenton process. Environ Sci Pollut Res 23:19134–19144CrossRefGoogle Scholar
  9. Bouafia-Chergui S, Khalaf H, Oturan N, Oturan MA (2010) Parametric study on the effect of the ratios [H2O2]/[Fe3+] and [H2O2]/[substrate] on the photo-Fenton degradation of cationic azo dye Basic Blue 41. J Environ Sci Health, part A 45:622–629CrossRefGoogle Scholar
  10. Boughrara S (2009) Analyse de cycle de vie environnemental des médicaments, master thesis, Environmental Engineering department, Université M’hamed Bougara-Boumerdes, AlgeriaGoogle Scholar
  11. Brezis M, Rosen S, Silva P (1984) Polyene toxicity in renal medulla: injury mediated by transport activity. Sci 224:66–68CrossRefGoogle Scholar
  12. Brienza M, Ahmed MM, Escande A, Plantard G, Scrano L, Chiron S, Bufo SA, Goetz V (2014) Relevance of a photo-Fenton like technology based on peroxymonosulphate for 17 beta-estradiol removal from wastewater. Chem Eng J 257:191–199CrossRefGoogle Scholar
  13. Canonica S, Meunier L (2008) Photo transformation of selected pharmaceuticals during UV treatment of drinking water. Water Res 42:121–128CrossRefGoogle Scholar
  14. Chue L, Anastasio C (2005) Formation of hydroxyl radical from the photolysis of frozen hydrogen peroxide. J Phys Chem 109:6264–6271CrossRefGoogle Scholar
  15. Coria G, Perez T, Sires I, Brillas E, Nava JL (2018) Abatement of the antibiotic levofloxacin in a solar photoelectro-Fenton flow plant: modeling the dissolved organic carbon concentration-time relationship. Chemosphere 198:174–181CrossRefGoogle Scholar
  16. Diagne M, Oturan N, Oturan MA, Sirés I (2009) UV-C light-enhanced photo-Fenton oxidation of methyl parathion. Environ Chem Lett 7:261–265CrossRefGoogle Scholar
  17. Diez AM, Ribeiro AS, Sanroman MA, Pazos M (2018) Optimization of photo-Fenton process for the treatment of prednisolone. Environ Sci Pollut Res 25:27768–27782CrossRefGoogle Scholar
  18. Dirany A, Efremova Aaron S, Oturan N, Sirés I, Oturan MA, Aaron JJ (2011) Study of the toxicity of sulfamethoxazole and its degradation products in water by a bioluminescence method during application of the electro-Fenton treatment. Anal Bioanal Chem 400:353–360CrossRefGoogle Scholar
  19. Dirany A, Sirés I, Oturan N, Özcan A, Oturan MA (2012) Electrochemical treatment of sulfachloropyridazine: kinetics, reaction pathways, and toxicity evolution. Environ Sci Technol 46:4074–4082CrossRefGoogle Scholar
  20. Elosaily GA, Salem HM, Hassan A, Maxwell S, Ibrahim Z (2014) Formulation, in-vitro and in-vivo evaluation of nystatin topical gel. J Am Sci 10:6Google Scholar
  21. Farre M, Ferrer I, Ginebreda A et al (2001) Determination of drugs in surface water and wastewater samples by liquid chromatography–mass spectrometry: methods and preliminary results including toxicity studies with Vibrio fischeri. J Chromatogr A 938:187–197CrossRefGoogle Scholar
  22. Fdil F, Aaron JJ, Oturan N, Chaouch A, Oturan MA (2003) Dégradation photochimique d'herbicides chlorophenoxyalcanoïques en milieux aqueux. Revue des Sciences de l'Eau 16:123–142CrossRefGoogle Scholar
  23. Feng L, Van Hullebusch E, Rodrigo M, Esposito G, Oturan MA (2013) Removal of residual anti-inflammatory and analgesic pharmaceuticals from aqueous systems by electrochemical advanced oxidation processes. A review. Chem Eng J 228:944–964CrossRefGoogle Scholar
  24. Fenoll J, Flores P, Hellín P, Martínez C, Navarro S (2012) Photodegradation of eight miscellaneous pesticides in drinking water after treatment with semiconductor materials under sunlight at pilot plant scale. Chem Eng J 204–206:54–64CrossRefGoogle Scholar
  25. Fent K, Weston A, Caminada D (2006) Ecotoxicology of human pharmaceuticals. Review Aquat Toxicol 76:122–159CrossRefGoogle Scholar
  26. Giraldo-Aguirre A, Serna-Galvis E, Erazo-Erazo E (2017) Removal of β-lactam antibiotics from pharmaceutical wastewaters using photo-Fenton process at near-neutral pH. Environ Sci Pollut Res 25:20293–20303. CrossRefGoogle Scholar
  27. Gonzalez O, Sans C, Esplugas S, Malato S (2009) Application of solar advanced oxidation processes to the degradation of the antibiotic sulfamethoxazole. Photochem Photobiol Sci 7:1032–1039CrossRefGoogle Scholar
  28. Iervolino G, Vaiano V, Sannino D, Rizzo L, Sarno G, Ciambelli P, Isupova L (2015) Influence of operating conditions in the photo-Fenton removal of tartrazine on tructured catalysts. Chem Eng Trans 43:979–984Google Scholar
  29. Iervolino G, Vaiano V, Sannino D, Rizzo L, Sarno G, Galluzi A, Polichetti A, Pepe G, Camoglia P (2017) Hydrogen production from glucose degradation in water and wastewater treated by Ru-LaFeO3/Fe2O3 magnetic particles photocatalysis and heterogeneous photo-Fenton. Int J Hydrog Energy 43(J):2184–2196Google Scholar
  30. Iervolino G, Vaiano V, Palma V (2019) Enhanced removal of water pollutants by dielectric barrier discharge non-thermal plasma reactor. Sep Purif Technol 215:155–162CrossRefGoogle Scholar
  31. Jain B, Singh AK, Kim H, Lichtfouse E, Sharma VK (2018) Treatment of organic pollutants by homogeneous and heterogeneous Fenton reaction processes. Environ Chem Lett 16:947–967CrossRefGoogle Scholar
  32. Jia YF, Zhou L, Ferronato C, Yang X, Salvador A, Zeng C, Chovelon JM (2015) Photocatalytic degradation of atenolol in aqueous titanium dioxide suspensions: kinetics, intermediates and degradation pathways. J Photochem Photobiol A 254:35–44CrossRefGoogle Scholar
  33. Kesraoui Abdessalem A, Bellakhal N, Oturan N, Dachraoui M, Oturan MA (2010) Treatment of a mixture of three pesticides by photo- and electro-Fenton processes. Desalination 250:450–455CrossRefGoogle Scholar
  34. Klavarioti M, Mantzavinos D, Kassinos D (2009) Removal of residual pharmaceuticals from aqueous systems by advanced oxidation processes, a review. Environ Int 35:402–417CrossRefGoogle Scholar
  35. Kolpin DW, Furlong ET, Meyer MT, Thurman EM, Zaugg SD, Barber LB, Buxton HT (2002) Pharmaceuticals, hormones, and others organic wastewater contaminants in U.S. streams, 1999-2000: a national reconnaissance. Environ Sci Technol 36:1202–1211CrossRefGoogle Scholar
  36. Kümmerer K (2005) The presence of pharmaceuticals in the environment due to human use-present knowledge and future challenges. Environ Manag 90:2354–2366Google Scholar
  37. Kümmerer K (2008) Pharmaceuticals in the environment – a brief summary. In: Kümmerer K (ed) Pharmaceuticals in the Environment. Sources fate effects and risks, third ed. Springer, Berlin, Heidelberg, pp 3–21Google Scholar
  38. Kümmerer K, Steger-Hartman T, Meyer M (1997) Biodegradability of the anti-tumor agent ifosfamide and its occurrence in hospital effluents and communal sewage. Water Res 31:2705–2710CrossRefGoogle Scholar
  39. Legrini O, Oliveros E, Braun AM (1993) Photochemical processes for water treatment. Chem Rev 93:671–698CrossRefGoogle Scholar
  40. Lindsey ME, Meyer M, Thurman EM (2001) Analysis of trace levels of sulfonamide and tetracycline antimicrobials in groundwater and surface water using solid-phase extraction and liquid chromatography/mass spectrometry. Anal Chem 73:4640–4646CrossRefGoogle Scholar
  41. Litter M (2005) Introduction to photochemical advanced oxidation processes for water treatment. Handb Environ Chem 2(Part M):325–366CrossRefGoogle Scholar
  42. Liu Y, Xuexiang H, Yongsheng F, Dionysiou D (2016) Degradation kinetics and mechanism of oxytetracycline by hydroxyl radical-based advanced oxidation processes. Chem Eng J 284:1317–1327CrossRefGoogle Scholar
  43. Lutterbeck C, Baginska E, Machado E, Kümmerer K (2015) Removal of the anti-cancer drug methotrexate from water by advanced oxidation processes: aerobic biodegradation and toxicity studies after treatment. Chemosphere 141:290–296CrossRefGoogle Scholar
  44. Mendez-Arriaga F, Torres-Palma RA, Petrier C, Esplugas S, Gimenez J, Pulgarin C (2009) Msineralization enhancement of a recalcitrant pharmaceutical pollutant in water by advanced oxidation hybrid processes. Water Res 43:3984–3991CrossRefGoogle Scholar
  45. Oller I, Malato S, Sanchez-Perez JA (2011) Combination of advanced oxidation processes and biological treatments for wastewater decontamination-a review. Sci Total Environ 409:4141–4166CrossRefGoogle Scholar
  46. Oturan MA, Aaron JJ (2014) Advanced oxidation processes in water/wastewater treatment: principles and applications. A review. Crit Rev Environ Sci Technol 44:2577–2641CrossRefGoogle Scholar
  47. Oturan N, Trajkovska S, Oturan MA, Couderchet M, Aaron JJ (2008) Study of the toxicity of diuron and its metabolites formed in aqueous medium during application of the electrochemical advanced oxidation process “electro-Fenton”. Chemosphere 73:1550–1556CrossRefGoogle Scholar
  48. Papoutsakis S, Pulgarin C, Oller I, Sanchez-Moreno R, Malato S (2016) Enhancement of the Fenton and photo-Fenton processes by components found in wastewater from the industrial processing of natural products: the possibilities of cork boiling wastewater reuse. Chem Eng J 304:890–896CrossRefGoogle Scholar
  49. Perez T, Sires I, Brillas E, Nava JL (2017) Solar photoelectro-Fenton flow plant modeling for the degradation of the antibiotic erythromycin in sulfate medium. Electrochim Acta 228:45–56CrossRefGoogle Scholar
  50. Pignatello JJ, Oliveros E, MacKay A (2006) Advanced oxidation processes for organic contaminant destruction based on the Fenton reaction and related chemistry. Crit Rev Environ Sci Technol 36:1–84CrossRefGoogle Scholar
  51. Pourakbar M, Moussavi G, Shekoohiyan S (2016) Homogenous VUV advanced oxidation process for enhanced degradation and mineralization of antibiotics in contaminated water. Ecotoxicol Environ Saf 125:72–77CrossRefGoogle Scholar
  52. Rastogi T, Leder C, Kümmerer K (2014) Qualitative environmental risk assessment of photolytic transformation products of iodinated X-ray contrast agent diatrizoic acid. Sci Total Environ 482-483:378–388CrossRefGoogle Scholar
  53. Romero V, Gonzalez O, Bayarri B, Marco P, Gimenez J, Esplugas S (2016) Degradation of metoprolol by photo-Fenton: comparison of different photoreactors performance. Chem Eng J 283:639–648CrossRefGoogle Scholar
  54. Schnell S, Bols NC, Barata C, Porte C (2009) Single and combined toxicity of pharmaceuticals and personal care products (PPCPs) on the rainbow trout liver cell line RTLW1. Aquat Toxicol 93:244–252CrossRefGoogle Scholar
  55. Sellers RM (1980) Spectrophotometric determination of hydrogen peroxide using potassium titanium (IV) oxalate. Analyst 105:950–954CrossRefGoogle Scholar
  56. Sirés I, Brillas E (2012) Remediation of water pollution caused by pharmaceutical residues based on electrochemical separation and degradation technologies: a review. Environ Int 40:212–229CrossRefGoogle Scholar
  57. Sörensen M, Zegenhagen F, Weckenmann J (2015) State of the art wastewater treatment in pharmaceutical and chemical industry by advanced oxidation. Pharm Ind 77:594–607Google Scholar
  58. Stief LJ, DeCarlo VJ (1969) Vacuum ultraviolet photochemistry. IX. Primary and chain processes in the photolysis of hydrogen peroxide. J Chem Phys 50:1234–1240CrossRefGoogle Scholar
  59. Sun JH, Sun SP, Fan MH, Guo HQ, Qiao LP, Sun RX (2007) A kinetic study on the degradation of p-nitroaniline by Fenton oxidation process. J Hazard Mater 148:172–177CrossRefGoogle Scholar
  60. Terns T, Stumpf M, Schuppert B et al (1998) Simultaneous determination of antiseptics and acidic drugs in sewage and river water. Vom Wasser 90:295–309Google Scholar
  61. Tijani J, Fatoba O, Madzivire G, Petrik L (2014) A review of combined advanced oxidation technologies for the removal of organic pollutants from water. Water Air Soil Pollut 225:2102–2132CrossRefGoogle Scholar
  62. Vione D, Minero C, Housari F, Chiron S (2007) Photoinduced transformation processes of 2,4-dichlorophenol and 2,6-dichlorophenol on nitrate irradiation. Chemosphere 69(10):1548–1554CrossRefGoogle Scholar
  63. Webb SF (2001) A data-based perspective on the environmental risk assessment of human pharmaceuticals I - collation of available ecotoxicity data. In: Kûmmerer K (ed) Pharmaceuticals in the environment - sources, fate, effects and risks. Springer-Verlag, Berlin, pp 175–201CrossRefGoogle Scholar
  64. Yingying X, Jingyun F, Chii S (2016) Kinetics and pathways of ibuprofen degradation by the UV/chlorine advanced oxidation process. Water Res 90:301–308CrossRefGoogle Scholar
  65. Zuccato E (2000) Presence of therapeutic drugs in the environment. Lancet 355:1789–1790CrossRefGoogle Scholar
  66. Zuorro A, Fidaleo M, Lavecchia R (2014) Degradation and antibiotic activity reduction of chloramphenicol in aqueous solution by UV/H2O2 process. J Environ Manag 133:302–308CrossRefGoogle Scholar

Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Laboratoire Génie de la Réaction, Faculté de Génie des Procédés et Génie MécaniqueU.S.T.H.B.Bab EzzouarAlgeria
  2. 2.Université Paris Est, Laboratoire Géomatériaux et Environnement, (EA 4508)UPEMMarne-la-ValléeFrance

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