Environmental Science and Pollution Research

, Volume 26, Issue 22, pp 23192–23197 | Cite as

24-Epibrassinolide pre-treatment reduces alkaline-induced oxidative stress in red rice seedlings

  • Mansi Sharma
  • Priyanka Mahajan
  • Harminder Pal SinghEmail author
  • Daizy Rani Batish
  • Ravinder Kumar Kohli
Short Research and Discussion Article


Soil alkalinity caused by salts, such as sodium bicarbonate (NaHCO3), and the frequently associated waterlogging problems are pervasive in agriculture and have a deleterious impact on crop production. However, various plant growth regulators, including brassinosteroids, are considered to be important against different abiotic stresses experienced by plants due to drought, salinity, and heavy metal stress. We investigated the putative role of 24-epibrassinolide (EBL), an active brassinosteroid, on red rice plants experiencing alkaline stress. Seedlings were pre-treated with 0.01 μM EBL for 30 min and later, exposed to NaHCO3 (25 mM) and were sampled, 5 days after treatments. Results showed that the pre-treatment of seedlings with EBL under non-stress conditions could promote rice plant growth. Growth parameters including dry weight (DW), root and coleoptile lengths were reduced under alkaline stress, whereas EBL application reduced the level of inhibition, as compared with NaHCO3 treatment. Enhanced levels of malondialdehyde content, hydrogen peroxide, and superoxide radicals were significantly diminished by EBL pre-treatment. Moreover, pre-treatment of EBL to alkaline-treated rice seedlings largely stimulated the enzymatic activities of ascorbate peroxidase, catalase, and superoxide dismutase. Thus, the results suggest that pre-application of EBL significantly ameliorates alkaline stress in rice.


Sodium bicarbonate Lipid peroxidation Superoxide anion Antioxidant enzymes Stress amelioration 



MS and PM are thankful to UGC for research fellowship.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflicts of interest.


  1. Abdel Latef AA, Tran LSP (2016) Impacts of priming with silicon on the growth and tolerance of maize plants to alkaline stress. Front Plant Sci 7:243CrossRefGoogle Scholar
  2. Ahmad P, Ozturk M, Sharma S, Gucel S (2014) Effect of sodium carbonate-induced salinity-alkalinity on some key osmoprotectants, protein profile, antioxidant enzymes, and lipid peroxidation in two mulberry (Morus alba L.) cultivars. J Plant Interact 9:460–467CrossRefGoogle Scholar
  3. Al-Mansouri HM, Alhendawi RAM (2014) Effect of increasing concentration of bicarbonate on plant growth and nutrient uptake by maize plants. Am Eurasian J Agric Environ Sci 14:1–6Google Scholar
  4. Anonymous (2018) Indigenous organic rice varieties from Eastern Himalayan region of Northeast, India. Nature Bio Foods Newsletters, July 28, 2018Google Scholar
  5. Bhat FM, Riar CS (2015) Health benefits of traditional rice varieties of temperate regions. Med Aromat Plants 4:198Google Scholar
  6. Cha-um S, Ashraf M, Kirdmanee C (2010) Screening upland rice (Oryza sativa L. ssp. indica) genotypes for salt-tolerance using multivariate cluster analysis. Afr J Biotechnol 9:4731–4740Google Scholar
  7. Chunthaburee S, Sakuanrungsirikul S, Wongwarat T, Sanitchon J, Pattanagul W, Theerakulpisut P (2016) Changes in anthocyanin content and expression of anthocyanin synthesis genes in seedlings of black glutinous rice in response to salt stress. Asian J Plant Sci 15:56–65CrossRefGoogle Scholar
  8. Dong Y, Wang W, Hu G, Chen W, Zhuge Y, Wang Z, He MR (2017) Role of exogenous 24-epibrassinolide in enhancing the salt tolerance of wheat seedlings. J Soil Sci Plant Nutr 17:554–569Google Scholar
  9. Gao Z, Lin Y, Wang X, Wei M, Yang F, Shi Q (2012) Sodium nitroprusside (SNP) alleviates the oxidative stress induced by NaHCO3 and protects chloroplast from damage in cucumber. Afr J Biotechnol 11:6974–6982Google Scholar
  10. Guo R, Shi LX, Ding XM, Hu Y, Tian SY, Yan DF, Shao S, Gao Y, Liu R, Yang YF (2010) Effects of saline and alkaline stress on germination, seedling growth, and ion balance in wheat. Agron J 102:1252–1260CrossRefGoogle Scholar
  11. Houimli SIM, Denden M, Mouhandes BD (2010) Effects of 24-epibrassinolide on growth, chlorophyll, electrolyte leakage and proline by pepper plants under NaCl-stress. EurAsian J BioSci 4:96–104CrossRefGoogle Scholar
  12. Huang B, DaCosta M, Jiang Y (2014) Research advances in mechanisms of turfgrass tolerance to abiotic stresses: from physiology to molecular biology. Crit Rev Plant Sci 33:141–189Google Scholar
  13. Kaur G, Singh HP, Batish DR, Kohli RK (2015) Adaptations to oxidative stress in Zea mays roots under short term Pb2+ exposure. Biologia 70:190–197Google Scholar
  14. Lee JA, Woolhouse HW (1969) A comparative study of bicarbonate inhibition of root growth in calcicole and calcifuge grasses. New Phytol 68:1–11CrossRefGoogle Scholar
  15. Mir MA, John R, Alyemeni MN, Alam P, Ahmad P (2018) Jasmonic acid ameliorates alkaline stress by improving growth performance, ascorbate-glutathione cycle and glyoxylase system in maize seedlings. Sci Rep 8:2831CrossRefGoogle Scholar
  16. Misra HR, Fridovich I (1972) The univalent reduction of oxygen by reduced flavins and quinines. J Biol Chem 247:188–192Google Scholar
  17. Munns R, Tester M (2008) Mechanisms of salinity tolerance. Annu Rev Plant Biol 59:651–681CrossRefGoogle Scholar
  18. Ogweno JO, Song XS, Shi K, Hu WH, Mao WH, Zhou YH (2008) Brassinosteroids alleviate heat-induced inhibition of photosynthesis by increasing carboxylation efficiency and enhancing antioxidant systems in Lycopersicon esculentum. J Plant Growth Regul 27:49–57CrossRefGoogle Scholar
  19. Peres ALGL, Soares JS, Tavares RG, Righetto G, Zullo MAT, Mandava NB, Menossi M (2019) Brassinosteroids, the sixth class of phytohormones: a molecular view from the discovery to hormonal interactions in plant development and stress adaptation. Int J Mol Sci 20:331CrossRefGoogle Scholar
  20. Planas-Riverolo A, Gupta A, Betegon-Putze I, Bosch N, Ibanes M, Cano-Delgado AI (2019) Brassinosteroid signalling in plant development and adaptation to stress. Development 146:dev151894Google Scholar
  21. Ramakrishna B, Rao SSR (2012) 24-Epibrassinolide alleviated zinc-induced oxidative stress in radish (Raphanus sativus L.) seedlings by enhancing antioxidative system. Plant Growth Regul 68:249–259CrossRefGoogle Scholar
  22. Rao SSR, Raghu K (2017) Effect of 24-epibrassinolide on growth and metabolism of rose-scented geranium [Pelargonium graveolens (L.) Herit] under cadmium toxicity. Int J Bot Stud 2:54–59Google Scholar
  23. Shang Q, Song S, Zhang Z, Guo S (2006) Exogenous brassinosteroid induced salt resistance of cucumber (Cucumis sativus L.) seedlings. Sci Agric Sin 39:1872–1877Google Scholar
  24. Shi D, Sheng Y (2005) Effect of various salt–alkaline mixed stress conditions on sunflower seedlings and analysis of their stress factors. Environ Exp Bot 54:8–21CrossRefGoogle Scholar
  25. Shi D, Wang D (2005) Effects of various salt-alkaline mixed stresses on Aneurolepidium chinense (Trin.) Kitag. Plant Soil 271:15–26CrossRefGoogle Scholar
  26. Singh HP, Batish DR, Kaur G, Arora K, Kohli RK (2008) Nitric oxide (as sodium nitroprusside) supplementation ameliorates Cd toxicity in hydroponically grown wheat roots. Environ Exp Bot 63:158–167Google Scholar
  27. Sun S, An M, Han L, Yin S (2015) Foliar application of 24-epibrassinolide improved salt stress tolerance of perennial ryegrass. Hort Sci 50:1518–1523CrossRefGoogle Scholar
  28. Surgun Y, Altunlu H, Turkekul S, Burun B, Yokas I (2015) Effects of 24-epibrassinolide on growth and some antioxidant enzymes of cotton (Gossypium hirsutum L.) cultivars under NaCl stress. J Appl Biol Sci 9:9–17Google Scholar
  29. Wang H, Wu Z, Chen Y, Yang C, Shi D (2011) Effects of salt and alkali stresses on growth and ion balance in rice (Oryza sativa L.). Plant Soil Environ 57:286–294CrossRefGoogle Scholar
  30. Wei LX, Lv BS, Wang MM, Ma HY, Yang HY, Liu XL, Jiang CJ, Liang ZW (2015) Priming effect of abscisic acid on alkaline stress tolerance in rice (Oryza sativa L.) seedlings. Plant Physiol Biochem 90:50–57CrossRefGoogle Scholar
  31. Wu W, Zhang Q, Ervin EH, Yang Z, Zhang X (2017) Physiological mechanism of enhancing salt stress tolerance of perennial ryegrass by 24-epibrassinolide. Front Plant Sci 8:1017CrossRefGoogle Scholar
  32. Yilmaz-Gokdogan E, Burun B (2017) The ameliorative effects of 24-epibrassinolide on shoot organogenesis inhibition occurring under NaCl-stressed conditions in cultures of cotyledon and hypocotyl explants of tomato (Lycopersicon esculentum Mill.). Acta Bot Croat 76:163–170CrossRefGoogle Scholar
  33. Zdemir F, Bor M, Demiral T, Türkan S (2004) Effects of 24-epibrassinolide on seed germination, seedling growth, lipid peroxidation, proline content and antioxidative system of rice (Oryza sativa L.) under salinity stress. Plant Growth Regul 42:203–211CrossRefGoogle Scholar

Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Department of Environment StudiesPanjab UniversityChandigarhIndia
  2. 2.Department of BotanyPanjab UniversityChandigarhIndia
  3. 3.Central University of PunjabBathindaIndia

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