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Environmental Science and Pollution Research

, Volume 26, Issue 22, pp 23192–23197 | Cite as

24-Epibrassinolide pre-treatment reduces alkaline-induced oxidative stress in red rice seedlings

  • Mansi Sharma
  • Priyanka Mahajan
  • Harminder Pal SinghEmail author
  • Daizy Rani Batish
  • Ravinder Kumar Kohli
Short Research and Discussion Article
  • 126 Downloads

Abstract

Soil alkalinity caused by salts, such as sodium bicarbonate (NaHCO3), and the frequently associated waterlogging problems are pervasive in agriculture and have a deleterious impact on crop production. However, various plant growth regulators, including brassinosteroids, are considered to be important against different abiotic stresses experienced by plants due to drought, salinity, and heavy metal stress. We investigated the putative role of 24-epibrassinolide (EBL), an active brassinosteroid, on red rice plants experiencing alkaline stress. Seedlings were pre-treated with 0.01 μM EBL for 30 min and later, exposed to NaHCO3 (25 mM) and were sampled, 5 days after treatments. Results showed that the pre-treatment of seedlings with EBL under non-stress conditions could promote rice plant growth. Growth parameters including dry weight (DW), root and coleoptile lengths were reduced under alkaline stress, whereas EBL application reduced the level of inhibition, as compared with NaHCO3 treatment. Enhanced levels of malondialdehyde content, hydrogen peroxide, and superoxide radicals were significantly diminished by EBL pre-treatment. Moreover, pre-treatment of EBL to alkaline-treated rice seedlings largely stimulated the enzymatic activities of ascorbate peroxidase, catalase, and superoxide dismutase. Thus, the results suggest that pre-application of EBL significantly ameliorates alkaline stress in rice.

Keywords

Sodium bicarbonate Lipid peroxidation Superoxide anion Antioxidant enzymes Stress amelioration 

Notes

Acknowledgements

MS and PM are thankful to UGC for research fellowship.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflicts of interest.

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Department of Environment StudiesPanjab UniversityChandigarhIndia
  2. 2.Department of BotanyPanjab UniversityChandigarhIndia
  3. 3.Central University of PunjabBathindaIndia

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