Environmental Science and Pollution Research

, Volume 26, Issue 18, pp 18465–18469 | Cite as

Thymoquinone ameliorates pulmonary vascular damage induced byEscherichia coliderived lipopolysaccharide via cytokine downregulation in rats

  • Naif A. Al-Gabri
  • Mohammed M. Qaid
  • Nahla H. El-shaer
  • Mutahar H. Ali
  • Alaeldein M. AbudabosEmail author
Research Article


Our study investigated the ameliorative effects of thymoquinone (TQ) on the pulmonary blood vessels which were injured after intratracheal administration of Escherichia coli–derived lipopolysaccharide (LPS) in a rat model. Forty rats (150 ± 50 g) were randomly divided into four groups equally. The first group was intratracheally administered LPS (Escherichia coli O55:B5) at a dose 200 μg. The second group was co-administered intraperitoneal injection of TQ and LPS daily for one week. The third group was provided intraperitoneal injection of 1 mg of TQ. The fourth group was administered normal saline intratracheally at the rate of 200 μl. The results revealed that cytokine level of interleukin-1 beta (IL-1β) and tumor necrosis factor alpha (TNFα) in serum was reduced in TQ-treated rats. Immunohistochemical study showed that expression of NF-kB was countered in the lung tissue by the application of TQ. In addition, the lesion score for various pathological aberrations were checked when rats were treated with TQ. From the results of the present study, it was concluded that TQ has an ameliorative effect on the pulmonary blood vascular damage via rearrangement of the cytokines in response to LPS injury in the rat model.


Arteriolar smooth muscles Lipopolysaccharide Rats Thymoquinone 


Funding information

This research received funding from the Deanship of Scientific Research at King Saud University through the Research Group Project No. RGP-273.


  1. Abudabos AM, Alyemni AH, Dafalla YM, Khan RU (2018) The effect of phytogenics on growth traits, blood biochemical and intestinal histology in broiler chickens exposed to Clostridium perfringens challenge. Journal of Applied Animal Research 46:691–695Google Scholar
  2. Al-Muhsen S, Johnson JR, Hamid Q (2011) Remodeling in asthma. J Allergy Clin Immunol 128:451–462CrossRefGoogle Scholar
  3. Banerjee S, Kaseb AO, Wang Z, Kong D, Mohammad M, Padhye S, Sarkar FH, Mohammad RM (2009) Antitumor activity of gemcitabine and oxaliplatin is augmented by thymoquinone in pancreatic cancer. Cancer Res 69:5575–5583CrossRefGoogle Scholar
  4. Bargi R, Asgharzadeh F, Beheshti F, Hosseini M, Sadeghnia HR, Khazaei M (2017) The effects of thymoquinone on hippocampal cytokine level, brain oxidative stress status and memory deficits induced by lipopolysaccharide in rats. Cytokine 96:173–184CrossRefGoogle Scholar
  5. Chehl N, Chipitsyna G, Gong Q, Yeo CJ, Arafat HA (2009) Anti-inflammatory effects of the Nigella sativa seed extract, thymoquinone, in pancreatic cancer cells. HPB (Oxf) 11:373–381CrossRefGoogle Scholar
  6. Darakhshan S, Pour AB, Colagar AH, Sisakhtnezhad S (2015) Thymoquinone and its therapeutic potentials. Pharmacol Res 95:138–158CrossRefGoogle Scholar
  7. El Aziz AE, El Sayed NS, Mahran LG (2011) Anti-asthmatic and anti-allergic effects of thymoquinone on airway-induced hypersensitivity in experimental animals. J Applied Pharmaceut Sci 1:109–117Google Scholar
  8. El Gazzar M, El Mezayen R, Nicolls MR, Marecki JC, Dreskin SC (2006) Down-regulation of leukotriene biosynthesis by thymoquinone attenuates airway inflammation in a mouse model of allergic asthma. Biochim Biophys Acta 1760:1088–1095CrossRefGoogle Scholar
  9. El Mezayen R, El Gazzar M, Nicolls MR, Marecki JC, Dreskin SC, Nomiyama H (2006) Effect of thymoquinone on cyclooxygenase expression and prostaglandin production in a mouse model of allergic airway inflammation. Immunol Lett 106:72–81CrossRefGoogle Scholar
  10. Gately S, Kerbel R (2003) Therapeutic potential of selective cyclooxygenase-2 inhibitors in the management of tumor angiogenesis. Prog Exp Tumor Res 37:179–192CrossRefGoogle Scholar
  11. Hou Y, Zhang H, Xie G, Cao X, Zhao Y, Liu Y, Mao Z, Yang J, Wu C (2013) Neuronal injury, but not microglia activation, is associated with ketamine-induced experimental schizophrenic model in mice. Prog Neuropsychopharmacol Biol Psychiatry 45:107–116Google Scholar
  12. Johnson BS, Matthay MA (2010) Acute lung injury: epidemiology, pathogenesis, and treatment. J Aer Med Pulm Drug Deliv 23:243–252CrossRefGoogle Scholar
  13. Knobloch J, Feldmann M, Wahl C, Jungck D, Behr J, Stoelben E, Koch A (2013) Endothelin receptor antagonists attenuate the inflammatory response of human pulmonary vascular smooth muscle cells to bacterial endotoxin. J Pharmacol Exp Ther 346:290–299CrossRefGoogle Scholar
  14. Kundu J, Chun KS, Aruoma OI, Kundu JK (2014) Mechanistic perspectives on cancer chemoprevention/chemotherapeutic effects of thymoquinone. Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis 768:22–34Google Scholar
  15. Płóciennikowska A, Hromada-Judycka A, Borzęcka K, Kwiatkowska K (2015) Cooperation of TLR4 and raft proteins in LPS-induced pro-inflammatory signaling. Cell Mol Life Sci 72:557–581CrossRefGoogle Scholar
  16. Rivera S, Liu L, Nemeth E, Gabayan V, Sorensen OE, Ganz T (2005) Hepcidin excess induces the sequestration of iron and exacerbates tumor-associated anemia. Blood 105(4):1797–1802Google Scholar
  17. Salem ML (2005) Immunomodulatory and therapeutic properties of the Nigella sativa L. seed. Int Immunopharmacol 5:1749–1770CrossRefGoogle Scholar
  18. Umar S, Zargan J, Umar K, Ahmad S, Katiyar CK, Khan HA (2012) Modulation of the oxidative stress and inflammatory cytokine response by thymoquinone in the collagen induced arthritis in Wistar rats. Chem Biol Interact 197:40–46CrossRefGoogle Scholar
  19. Woo CC, Kumar AP, Sethi G, Tan KH (2012) Thymoquinone: potential cure for inflammatory disorders and cancer. Biochem Pharmacol 83:443–451CrossRefGoogle Scholar
  20. Zhu N, Zhao X, Xiang Y, Ye S, Huang J, Hu W, Lv L, Zeng C (2016) Thymoquinone attenuates monocrotaline-induced pulmonary artery hypertension via inhibiting pulmonary arterial remodeling in rats. Int J Cardiol 221:587–596CrossRefGoogle Scholar

Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  • Naif A. Al-Gabri
    • 1
    • 2
  • Mohammed M. Qaid
    • 2
    • 3
  • Nahla H. El-shaer
    • 4
  • Mutahar H. Ali
    • 3
  • Alaeldein M. Abudabos
    • 3
    Email author
  1. 1.Department of Pathology, Faculty of Veterinary MedicineZagazig UniversityZagazigEgypt
  2. 2.Veterinary Department, Faculty of Agriculture and Veterinary MedicineThamar UniversityDhamarYemen
  3. 3.Department of Animal Production, College of Food and Agriculture SciencesKing Saud UniversityRiyadhKingdom of Saudi Arabia
  4. 4.Zoology Department, Faculty of ScienceZagazig UniversityZagazigEgypt

Personalised recommendations