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Environmental Science and Pollution Research

, Volume 26, Issue 18, pp 18465–18469 | Cite as

Thymoquinone ameliorates pulmonary vascular damage induced byEscherichia coliderived lipopolysaccharide via cytokine downregulation in rats

  • Naif A. Al-Gabri
  • Mohammed M. Qaid
  • Nahla H. El-shaer
  • Mutahar H. Ali
  • Alaeldein M. AbudabosEmail author
Research Article
  • 95 Downloads

Abstract

Our study investigated the ameliorative effects of thymoquinone (TQ) on the pulmonary blood vessels which were injured after intratracheal administration of Escherichia coli–derived lipopolysaccharide (LPS) in a rat model. Forty rats (150 ± 50 g) were randomly divided into four groups equally. The first group was intratracheally administered LPS (Escherichia coli O55:B5) at a dose 200 μg. The second group was co-administered intraperitoneal injection of TQ and LPS daily for one week. The third group was provided intraperitoneal injection of 1 mg of TQ. The fourth group was administered normal saline intratracheally at the rate of 200 μl. The results revealed that cytokine level of interleukin-1 beta (IL-1β) and tumor necrosis factor alpha (TNFα) in serum was reduced in TQ-treated rats. Immunohistochemical study showed that expression of NF-kB was countered in the lung tissue by the application of TQ. In addition, the lesion score for various pathological aberrations were checked when rats were treated with TQ. From the results of the present study, it was concluded that TQ has an ameliorative effect on the pulmonary blood vascular damage via rearrangement of the cytokines in response to LPS injury in the rat model.

Keywords

Arteriolar smooth muscles Lipopolysaccharide Rats Thymoquinone 

Notes

Funding information

This research received funding from the Deanship of Scientific Research at King Saud University through the Research Group Project No. RGP-273.

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  • Naif A. Al-Gabri
    • 1
    • 2
  • Mohammed M. Qaid
    • 2
    • 3
  • Nahla H. El-shaer
    • 4
  • Mutahar H. Ali
    • 3
  • Alaeldein M. Abudabos
    • 3
    Email author
  1. 1.Department of Pathology, Faculty of Veterinary MedicineZagazig UniversityZagazigEgypt
  2. 2.Veterinary Department, Faculty of Agriculture and Veterinary MedicineThamar UniversityDhamarYemen
  3. 3.Department of Animal Production, College of Food and Agriculture SciencesKing Saud UniversityRiyadhKingdom of Saudi Arabia
  4. 4.Zoology Department, Faculty of ScienceZagazig UniversityZagazigEgypt

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