Profiling of main metabolites in root exudates and mucilage collected from maize submitted to cadmium stress

Lapie, Clémentine; Leglize, Pierre; Paris, Cédric; Buisson, Tatiana; Sterckeman, Thibault

doi:10.1007/s11356-019-05168-0

Research Article
Published: 25 April 2019

Profiling of main metabolites in root exudates and mucilage collected from maize submitted to cadmium stress

Clémentine Lapie¹,
Pierre Leglize ORCID: orcid.org/0000-0002-8646-795X¹,
Cédric Paris^2,3,
Tatiana Buisson¹ &
Thibault Sterckeman¹

Environmental Science and Pollution Research volume 26, pages 17520–17534 (2019)Cite this article

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Abstract

The aim of this study was to characterize qualitatively and quantitatively the composition of the main rhizodeposits emitted from maize (Zea mays) under Cd stress, in order to discuss their role in Cd availability and tolerance. Maize was grown for 6 weeks in sand at four Cd exposure levels (0, 10, 20, and 40 μM Cd in nutrient solution) and two types of rhizodeposits were collected at the end of cultivation period. Mucilage and other molecules adhering to rhizospheric sand were extracted with a buffer before root exudates were collected by diffusion into water. Total carbon, proteins, amino acids, and sugars were analyzed for both rhizodeposit types and about 40 molecules were identified using GC-MS and LC-MS. Cadmium effect on plant morphology and functioning was slight, but consistent with previous works on Cd toxicity. However, rhizodeposition did tend to be impacted, with a decrease in total carbon, sugars, and amino acids correlating with an increasing Cd content. Such a decrease was not noticeable for proteins in root exudates. These observations were confirmed by the same trends in individual compound contents, although the results were generally not statistically significant. Many of the molecules determined are well-known to modify, whether directly or indirectly, Cd speciation and dynamics in the soil and could play a role in Cd tolerance.

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Acknowledgements

Molecular analyses were performed at the “Plateau d’Analyse Structurale et Métabolomique” of the “Ecole Nationale Supérieure d’Agronomie et des Industries Alimentaires” by using GCMS and LCMS equipment. We would like to acknowledge the technical team of the “Laboratoire Sols et Environnement” for their help and support and especially Jean-Claude Begin for the plant grinding. We are thankful to Jérémy Grosjean and Christophe Robin from the “Laboratoire Agronomie et Environnement”, Claire Soligot from the “Unité de Recherche Animal et Fonctionnalités des Produits Animaux,” and Catherine Lorgeoux from the “Laboratoire Georessources” for their advice and technical assistance.

Funding

This work was financially supported by the “Ministère de l’Enseignement Supérieur et de la Recherche” with a three-year doctoral scholarship.

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Affiliations

Laboratoire Sols et Environnement, Université de Lorraine, Inra, F-54000, Nancy, France
Clémentine Lapie, Pierre Leglize, Tatiana Buisson & Thibault Sterckeman
Laboratoire d’Ingénierie des Biomolécules, Université de Lorraine, F-54000, Nancy, France
Cédric Paris
Plateau d’Analyse Structurale et Métabolomique, SF4242, EFABA, Université de Lorraine, Vandœuvre-lès-Nancy, France
Cédric Paris

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Clémentine Lapie
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Pierre Leglize
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Cédric Paris
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Tatiana Buisson
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Thibault Sterckeman
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Correspondence to Pierre Leglize.

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Lapie, C., Leglize, P., Paris, C. et al. Profiling of main metabolites in root exudates and mucilage collected from maize submitted to cadmium stress. Environ Sci Pollut Res 26, 17520–17534 (2019). https://doi.org/10.1007/s11356-019-05168-0

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Received: 18 December 2018
Accepted: 11 April 2019
Published: 25 April 2019
Issue Date: 01 June 2019
DOI: https://doi.org/10.1007/s11356-019-05168-0

Keywords

Root exudation
Mucilage
Organic analysis
Sugar
Polysaccharide
Amino acid
Heavy metal