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Environmental Science and Pollution Research

, Volume 26, Issue 18, pp 18181–18190 | Cite as

Metabonomic analysis of the hepatic injury suffer from hexavalent chromium poisoning in broilers

  • Yali Zhao
  • Hui Zhang
  • Xiaoxing Wu
  • Tianguang Zhang
  • Ke Shen
  • Lei Li
  • Yuxuan Peng
  • Khalid Mehmood
  • Donghai ZhouEmail author
Research Article
  • 137 Downloads

Abstract

Chromium is used in daily life and has a wide range of functions. It plays an important role in protein synthesis and carbohydrate and lipid metabolism. Chromium is found in trivalent Cr(III) and hexavalent Cr(VI) form; Cr(III) is relatively stable and intimately participates with many phenomena of metabolisms. Whereas, Cr(VI) is toxic, which results in growth inhibition and leading to changes in components of antioxidant systems as well as secondary metabolites. However, the molecular mechanism that is involved in Cr (VI)-induced hepatotoxicity is still unclear. For this purpose, 40 chickens were randomly assigned into two groups: the normal group (feeding the basic diet and clear water), the chromium group (16%LD50, 74.24 mg/kg/day K2Cr2O7). The samples were subjected to pathological examination and UHPLC-QE-MS non-target metabolomics method for metabolomics analysis of broiler liver using principal component analysis (PCA) and partial least squares discriminant analysis (OPLS-DA). The central venous cells of the broiler liver in the chromium poisoning group showed turbidity and flaky necrosis, nuclear condensation, nuclear rupture, and even nuclear dissolution. The differential metabolite analysis between the chromium poisoning and the control group showed that 32 differential metabolites were upregulated and 15 were downregulated in positive ion mode. Whereas,17 differential metabolites were downregulated, and 35 were downregulated in negative ion mode (P ≤ 0.05). The potential marker substances are oleic acidamide, farnesylacetone, betaine, taurine, choline, and galactinol. Additionally, Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways showed that the lipid metabolism, carbohydrate metabolism, nucleotide metabolism, amino acid metabolism, energy metabolism, membrane transport, digestive system, and nervous system were the most important metabolic pathways in the liver. This study provides a theoretical basis for the future understanding of the pathogenesis of chromium poisoning and a new insight of the subsequent molecular mechanism of chromium hepatotoxicity.

Keywords

Chromium poisoning UHPLC-QE-MS metabolomics Metabolites Hepatotoxicity Differential metabolites 

Notes

Author contributions

Y.Z., X.W., H.Z., T.Z., K.S., L.L., K.M., Y.P., and D.Z. were responsible for study conception and design; H.Z. and K.M. were involved in the drafting of the manuscript.

Funding information

This study was supported by the National Key R&D Program of China (2016YFD0501208), National Natural Science Foundation of China (no. 30700588), and Hubei Provincial Natural Science Foundation of China (grant no. 2014CFB244).

Compliance with ethical standards

All the experiments were performed after the approval of the Institutional Animal Welfare and Research Ethics Committee of Huazhong Agricultural University Wuhan, China (approval no. 31272556). All animal experiments and methods were conducted under the relevant procedure of Proclamation of the Standing Committee of Hubei People’s Congress (no. 29), China.

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

11356_2019_5075_MOESM1_ESM.docx (12 kb)
ESM 1 (DOCX 12 kb)

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  • Yali Zhao
    • 1
  • Hui Zhang
    • 1
    • 2
  • Xiaoxing Wu
    • 1
  • Tianguang Zhang
    • 1
  • Ke Shen
    • 1
  • Lei Li
    • 1
  • Yuxuan Peng
    • 1
  • Khalid Mehmood
    • 1
    • 3
  • Donghai Zhou
    • 1
    Email author
  1. 1.College of Veterinary MedicineHuazhong Agricultural UniversityWuhanP.R. China
  2. 2.College of Veterinary MedicineCornell UniversityIthacaUSA
  3. 3.University College of Veterinary and Animal SciencesIslamia University of BahawalpurBahawalpurPakistan

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