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Protective effect of Astragaloside IV to inhibit thiram-induced tibial dyschondroplasia

  • Khalid Mehmood
  • Hui Zhang
  • Wangyuan Yao
  • Xiong Jiang
  • Muhammad Waqas
  • Aoyun Li
  • Yaping Wang
  • Li Lei
  • Lihong Zhang
  • Hammad Qamar
  • Jiakui LiEmail author
Research Article
  • 40 Downloads

Abstract

Tibial dyschondroplasia (TD) is most the common tibiotarsal bone disease in rapidly growing birds throughout the world. There is accumulating evidence that COX-2 abnormal expression in tibia plays an important role in TD progression. So, the regulation of COX-2 is an ever more appealing target for therapeutic intervention in TD. Astragaloside IV has an indispensable role in maintaining COX-2 expression in many diseases. So, we designed this study to use Astragaloside IV (AST-IV) against TD-affected chickens. A total of 180 Arbor Acres chickens were randomly divided in the control group, TD group, and Astr (AST-IV-treated chickens) group. During the experiment, mortality, feed conversion ratio, physiological changes, biochemical criterion, liver antioxidant enzymes, and gene expression of COX-2 were examined in all the chicken groups at various days. The results showed that AST-IV administration restored the growth performance and tibia lesions and decreased the mortality as compared with TD chickens. The biochemical criterion (ALP, AST, and ALT) of serum and liver antioxidant enzymes (SOD, GSH-Px, MDA, and T-AOC) improved after the administration of AST-IV. The COX-2 gene was upregulated significantly (P < 0.05) in TD chickens. Whereas, AST-IV treatment downregulated both gene and protein expression of COX-2 significantly (P < 0.05) in TD-affected chickens. AST-IV recovered tibial dyschondroplasia chickens by increasing the growth performance, ameliorating tibial cartilage damage, and decreasing COX-2 expression. In conclusion, AST-IV can be used to prevent thiram-induced TD in chickens.

Keywords

Tibial dyschondroplasia (TD) Thiram Astragaloside IV COX-2 Chickens 

Notes

Funding information

The study was supported by the National Key R&D Program of China (Project no. 2017YFD0502200).

Compliance with ethical standards

Animals experiment was conducted under the Institutional Animal Care and Use committees of Huazhong Agricultural University approval (31272517), China.

Conflict of interest

The authors declare that they have no competing interest.

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  1. 1.College of Veterinary MedicineHuazhong Agricultural UniversityWuhanPeople’s Republic of China
  2. 2.College of Animals Husbandry and Veterinary MedicineTibet Agricultural and Animal Husbandry UniversityLinzhiPeople’s Republic of China
  3. 3.University College of Veterinary & Animal SciencesIslamia University of BahawalpurBahawalpurPakistan

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