Biocementation of hazardous waste is used in reducing the mobility of contaminants, but studies on evaluating its efficacy have not been well documented. Therefore, to evaluate the efficacy of this method, physicochemical factors affecting stabilized hazardous products of in situ microbially induced calcium carbonate precipitation (MICP) were determined. The strength and leach resistance were investigated using the bacterium Pararhodobacter sp. Pb-contaminated kiln slag (KS) and leach plant residue (LPR) collected from Kabwe, Zambia, were investigated. Biocemented KS and KS/LPR had leachate Pb concentrations below the detection limit of < 0.001 mg/L, resisted slaking, and had maximum unconfined compressive strengths of 8 MPa for KS and 4 MPa for KS/LPR. Furthermore, biocemented KS and KS/LPR exhibited lower water absorption coefficient values, which could potentially reduce the water transportation of Pb2+. The results of this study show that MICP can reduce Pb2+ mobility in mine wastes. The improved physicochemical properties of the biocemented materials, therefore, indicates that this technique is an effective tool in stabilizing hazardous mine wastes and, consequently, preventing water and soil contamination.
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Achal V, Pan X, Lee DJ, Kumari D, Zhang D (2013) Remediation of Cr (VI) from chromium slag by biocementation. Chemosphere 93:1352–1358. https://doi.org/10.1016/j.chemosphere.2013.08.008
Akbar A, Sinegani S, Monsef MJ (2016) Chemical speciation and bioavailability of cadmium in the temperate and semiarid soils treated with wheat residue. Environ Sci Pollut Res 23:9750–9758. https://doi.org/10.1007/s11356-016-6171-x
Al-Kindi G (2019) Evaluation the solidification/stabilization of heavy metals by Portland cement. J Ecol Eng 20:91–100. https://doi.org/10.12911/22998993/99739
Ashrafi M, Mohamad S, Yusoff I, Hamid FS (2015) Immobilization of Pb, Cd, and Zn in a contaminated soil using eggshell and banana stem amendments: metal leachability and a sequential extraction study. Environ Sci Pollut Res 22:223–230. https://doi.org/10.1007/s11356-014-3299-4
ASTM International (2003) ASTM D5084-03 standard test methods for measurement of hydraulic conductivity of saturated porous materials using a flexible wall permeameter
ASTM International (2013) ASTM C1585-13 standard test method for measurement of rate of absorption of water by hydraulic-cement concretes
ASTM International (2016) ASTM D4644-16 standard test method for slake durability of shales and other similar weak rocks
Bates E, Hills C (2015) Stabilization and solidification of contaminated soil and waste: a manual of practice
Blacksmith I (2013) The worlds worst 2013: the top ten toxic threats. Zurich
Chen X, Guo H, Cheng X (2017) Heavy metal immobilisation and particle cementation of tailings by biomineralisation. Environ Geotech:1–7. https://doi.org/10.1680/jenge.15.00068
Cheng L, Cord-Ruwisch R (2014) Upscaling effects of soil improvement by microbially induced calcite precipitation by surface percolation. Geomicrobiol J 31:396–406. https://doi.org/10.1080/01490451.2013.836579
Cheng L, Cord-Ruwisch R, Shahin MA (2013) Cementation of sand soil by microbially induced calcite precipitation at various degrees of saturation. Can Geotech J 50:81–90. https://doi.org/10.1139/cgj-2012-0023
Demirci EE, Sahin R (2014) Effect of strength class of concrete and curing conditions on capillary water absorption of self-compacting and conventional concrete. Int J Civ Environ Eng 8:1191–1198
Dhami NK, Reddy MS, Mukherjee A (2016) Significant indicators for biomineralisation in sand of varying grain sizes. Constr Build Mater 104:198–207. https://doi.org/10.1016/j.conbuildmat.2015.12.023
Doostmohammadi R, Olfati M, Roodsari FG (2017) Mining pollution control using biogrouting. J Min Sci 53:367–376. https://doi.org/10.1134/S1062739117022248
Eryürük K, Yang S, Suzuki D, Sakaguchi I, Akatsuka T, Tsuchiya T, Katayama A (2015) Reducing hydraulic conductivity of porous media using CaCO3 precipitation induced by Sporosarcina pasteurii. J Biosci Bioeng 119:331–336. https://doi.org/10.1016/j.jbiosc.2014.08.009
Gutiérrez M, Mickus K, Camacho LM (2016) Abandoned Pb/Zn mining wastes and their mobility as proxy to toxicity: a review. Sci Total Environ 565:392–400. https://doi.org/10.1016/j.scitotenv.2016.04.143
Huang G, Su X, Rizwan MS, Zhu Y, Hu H (2016) Chemical immobilization of Pb, Cu, and Cd by phosphate materials and calcium carbonate in contaminated soils. Environ Sci Pollut Res 23:16845–16856. https://doi.org/10.1007/s11356-016-6885-9
International Society for Rock Mechanics (2015) The ISRM suggested methods for rock characterization, testing and monitoring: 2007–2014. Springer International Publishing, Cham
Ivanov V, Stabnikov V (2016) Construction biotechnology: biogeochemistry, microbiology and biotechnology of construction materials and processes. Springer
Jaishankar M, Tseten T, Anbalagan N, Mathew BB, Beeregowda KN (2014) Toxicity, mechanism and health effects of some heavy metals. Interdiscip Toxicol 7:60–72. https://doi.org/10.2478/intox-2014-0009
Jena S, Dey SK (2016) Heavy metals. Am J Environ Stud 1:48–60
Karagiannis N, Karoglou M, Bakolas A, Moropoulou A (2016) New approaches to building pathology and durability. New Approaches to Build Pathol Durab 6:27–44. https://doi.org/10.1007/978-981-10-0648-7
Kim JH, Lee JY (2018) An optimum condition of MICP indigenous bacteria with contaminated wastes of heavy metal. J Mater Cycles Waste Manag 21:1–9. https://doi.org/10.1007/s10163-018-0779-5
Kříbek B, Nyambe I, Majer V, Knésl I, Mihaljevič M, Ettler V, Vaněk A, Penížek V, Sracek O (2019) Soil contamination near the Kabwe Pb-Zn smelter in Zambia: environmental impacts and remediation measures proposal. J Geochemical Explor 197:159–173. https://doi.org/10.1016/j.gexplo.2018.11.018
Li D, Zeng L, Jiao B et al (2018) Solidification/stabilization of lead-zinc smelting slag in composite based geopolymer. J Clean Prod 209:1206–1215. https://doi.org/10.1016/j.jclepro.2018.10.265
Liu S, Tian S, Li K, Wang L, Liang T (2018) Heavy metal bioaccessibility and health risks in the contaminated soil of an abandoned, small-scale lead and zinc mine. Environ Sci Pollut Res 25:15044–15056. https://doi.org/10.1007/s11356-018-1660-8
Mujah D, Shahin MA, Cheng L (2017) State-of-the-art review of biocementation by microbially induced calcite precipitation (MICP) for soil stabilization. Geomicrobiol J 34:524–537. https://doi.org/10.1080/01490451.2016.1225866
Nam IH, Roh SB, Park MJ, Chon CM, Kim JG, Jeong SW, Song H, Yoon MH (2016) Immobilization of heavy metal contaminated mine wastes using Canavalia ensiformis extract. Catena 136:53–58. https://doi.org/10.1016/j.catena.2015.07.019
Ng W, Lee M, Hii S (2012) An overview of the factors affecting microbial-induced calcite precipitation and its potential application in soil improvement. World Acad Sci Eng Technol 62:723–729
Pan Y, Rossabi J, Pan C, Xie X (2019) Stabilization/solidification characteristics of organic clay contaminated by lead when using cement. J Hazard Mater 362:132–139. https://doi.org/10.1016/j.jhazmat.2018.09.010
Ren XW, Santamarina JC (2017) The hydraulic conductivity of sediments: a pore size perspective. Eng Geol 233:48–54. https://doi.org/10.1016/j.enggeo.2017.11.022
Rowshanbakht K, Khamehchiyan M, Sajedi RH, Nikudel MR (2016) Effect of injected bacterial suspension volume and relative density on carbonate precipitation resulting from microbial treatment. Ecol Eng 89:49–55. https://doi.org/10.1016/j.ecoleng.2016.01.010
Tang W, Shan B, Zhang H, Zhu X, Li S (2016) Heavy metal speciation, risk, and bioavailability in the sediments of rivers with different pollution sources and intensity. Environ Sci Pollut Res 23:23630–23637. https://doi.org/10.1007/s11356-016-7575-3
Team R. Core (2018) R: a language and environment for statistical computing, p 201
Tsang DCW, Yip ACK, Olds WE, Weber PA (2014) Arsenic and copper stabilisation in a contaminated soil by coal fly ash and green waste compost. Environ Sci Pollut Res 21:10194–10204. https://doi.org/10.1007/s11356-014-3032-3
U.S. EPA (2017) Leaching Environmental Assessment Framework (LEAF) how-to guide understanding the LEAF approach and how and when to use it
Wang Z, Zhang N, Ding J, Lu C, Jin Y (2018) Experimental study on wind erosion resistance and strength of sands treated with microbial-induced calcium carbonate precipitation. Adv Mater Sci Eng 2018:1–10. https://doi.org/10.1155/2018/3463298
Yabe J, Nakayama SMM, Ikenaka Y, Muzandu K, Ishizuka M, Umemura T (2011) Uptake of lead, cadmium, and other metals in the liver and kidneys of cattle near a lead-zinc mine in Kabwe, Zambia. Environ Toxicol Chem 30:1892–1897. https://doi.org/10.1002/etc.580
Yabe J, Nakayama SMM, Ikenaka Y, Yohannes YB, Bortey-Sam N, Kabalo AN, Ntapisha J, Mizukawa H, Umemura T, Ishizuka M (2018) Lead and cadmium excretion in feces and urine of children from polluted townships near a lead-zinc mine in Kabwe, Zambia. Chemosphere 202:48–55. https://doi.org/10.1016/j.chemosphere.2018.03.079
Yutong Z, Qing X, Shenggao L (2016) Distribution, bioavailability, and leachability of heavy metals in soil particle size fractions of urban soils (northeastern China). Environ Sci Pollut Res 23:14600–14607. https://doi.org/10.1007/s11356-016-6652-y
ZEMA (2013) Limits for effluent and wastewater—Licencing Regulations 7(2) Third schedule
Zhang Y, Yao J, Wang T et al (2016a) Bioremediation of Cd by strain GZ-22 isolated from mine soil based on biosorption and microbially induced carbonate precipitation. Environ Sci Pollut Res 24:372–380. https://doi.org/10.1007/s11356-016-7810-y
Zhang Y, Zhang S, Wang R, Cai J, Zhang Y, Li H, Huang S, Jiang Y (2016b) Impacts of fertilization practices on pH and the pH buffering capacity of calcareous soil. Soil Sci Plant Nutr 62:432–439. https://doi.org/10.1080/00380768.2016.1226685
Zhu X, Li W, Zhan L, Huang M, Zhang Q, Achal V (2016) The large-scale process of microbial carbonate precipitation for nickel remediation from an industrial soil. Environ Pollut 219:149–155. https://doi.org/10.1016/j.envpol.2016.10.047
This work was partly supported by Japan International Cooperation Agency (JICA)/Japan Science and Technology Agency (JST), Science and Technology Research Partnership for Sustainable Development (SATREPS), and Japan Society for the Promotion of Science (JSPS) KAKENHI under grant numbers JP18H03395 and JP16H04404.
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Mwandira, W., Nakashima, K., Kawasaki, S. et al. Efficacy of biocementation of lead mine waste from the Kabwe Mine site evaluated using Pararhodobacter sp.. Environ Sci Pollut Res 26, 15653–15664 (2019). https://doi.org/10.1007/s11356-019-04984-8
- Abandoned mine