Environmental Science and Pollution Research

, Volume 26, Issue 12, pp 12302–12310 | Cite as

Histopathological and biochemical studies on the effect of curcumin and taurine against bisphenol A toxicity in male rats

  • Fatma Gökçe ApaydinEmail author
  • Ayşe Aslanturk
  • Meltem Uzunhisarcikli
  • Hatice Bas
  • Suna Kalender
  • Yusuf Kalender
Research Article


Bisphenol A (BPA) is a chemical found in environmental xenoestrogen. In the present study, olive oil, curcumin, taurine, BPA, curcumin plus BPA, and taurine plus BPA were exposed to rats for 4 weeks via gavage. Content of malondialdehyde and activities of antioxidant enzymes (GPx, GST, SOD, CAT) and also histopathological and cytopathological changes of heart were studied. No significant changes in all studied parameters were seen between control, olive oil, curcumin, and taurine-treated groups. However, there were significant differences in levels of malondialdehyde and activities of antioxidant enzymes in BPA-exposed rats and some histo/cytopathological changes determined. In curcumin plus BPA-exposed and taurine plus BPA-exposed groups, we measured the preventive effects on some parameters but not exactly. As a result, curcumin and taurine significantly minimized BPA-induced cardiotoxicity in rats.


Bisphenol A Curcumin Taurine Electron microscopy Light microscopy Heart 


Compliance with ethical standards

Experimental studies were confirmed by University of Gazi Animal Ethics Committee (G.U.ET–14.075).

Conflict of interest

The authors declare that they have no conflict of interest.


  1. Aebi H (1984) Catalase in vitro. Methods Enzymol 105:121–126. CrossRefGoogle Scholar
  2. Agha FE, Youness ER, Selim MHH (2014) Nephroprotective potential of selenium and taurine against mercuric chloride induced nephropathy in rats. Ren Fail 36:704–716. CrossRefGoogle Scholar
  3. Akingbemi BT, Sottas CM, Koulova AI, Klinefelter GR, Hardy MP (2004) Inhibition of testicular steroidogenesis by the xenoestrogen bisphenol A is associated with reduced pituitary luteinizing hormone secretion and decreased steroidogenic enzyme gene expression in rat Leydig cells. Endocrinology 145(2):592–603. CrossRefGoogle Scholar
  4. Apaydın FG, Bas H, Kalender S, Kalender Y (2016) Subacute effects of low dose lead nitrate and mercury chloride exposure on kidney of rats. Environ Toxicol Pharmacol 41:219–224. CrossRefGoogle Scholar
  5. Apaydın FG, Bas H, Kalender S, Kalender Y (2017) Bendiocarb induced histopathological and biochemical alterations in rat liver and preventive role of vitamins C and E. Environ Toxicol Pharmacol 49:148–155. CrossRefGoogle Scholar
  6. Apaydın FG, Uzunhisarcıklı M, Aslantürk A, Kalender S (2018) Bisphenol A-induced histopathological alterations on small intestine tissues of rats: the protective role of taurine and curcumin. Iğdır Univ J Inst Sci Tech 8:43–47. CrossRefGoogle Scholar
  7. Baş H, Kalender Y (2011) Chlorpyrifos induced cardiotoxicity in rats and the protective role of quercetin and catechin. GU J Sci 24(3):387–395Google Scholar
  8. Baş H, Kalender S, Apaydın FG (2014) Adverse effects of lead treatment: relationship of histopathological changes and protective role of sodium selenite on non-diabetic and diabetic rat hearts. GU J Sci 27(2):855–859Google Scholar
  9. Baş H, Kalender S, Karaboduk H, Apaydın FG (2015) The effects on antioxidant enzyme systems in rat brain tissues of lead nitrate and mercury chloride. GU J Sci 28(2):169–174Google Scholar
  10. Berger A, Ziv-Gal A, Cudiamat J, Wang W, Zhou C, Flaws JA (2016) The effects of in utero bisphenol A exposure on the ovaries in multiple generations of mice. Reprod Toxicol 60:39–52. CrossRefGoogle Scholar
  11. Degirmenci I, Kalender S, Ustuner MC, Kalender Y, Gunes HV, Unal H, Basaran A (2002) The effects of acarbose and Rumex patientia on liver ultrastructure in streptozotocin-induced diabetic (type II) rats. Drug Exp Clin Res 28(6):229–234Google Scholar
  12. Degirmenci I, Ustuner MC, Kalender Y, Kalender S, Gunes HV (2005) The effects of acarbose and Rumex patientia L. on ultrastructural and biochemical changes of pancreatic B cells in streptozotocin-induced diabetic rats. J Ethnopharmacol 97:555–559. CrossRefGoogle Scholar
  13. Djuric A, Begic A, Gobeljic B, Stanojevic I, Ninkovic M, Vojvodic D, Pantelic A, Zebic G, Prokic V, Dejanovic B, Stojanovic I, Pavlica M, Djukic D, Saso L, Djurdjevic D, Pavlovii M, Topic A, Vujanovic D, Stevnovic I, Djukic M (2015) Oxidative stress, bioelements and androgen status in testes of rats subacutely exposed to cadmium. Food Chem Toxicol 86:25–33. CrossRefGoogle Scholar
  14. Eid JI, Eissa SM, El-Ghor AA (2015) Bispheol A induces oxidative stress and DNA damage in hepatic tissue of female rat offspring. JOBAZ 71:10–19. Google Scholar
  15. El Tabaa MM, Sokkar SS, Ramadan ES, Abd El Salam IZ, Zaid A (2017) Neuroprotective role of Ginkgo biloba against cognitive deficits associated with bisphenol a exposure: an animal model study. Neurochem Int 108:199–212. CrossRefGoogle Scholar
  16. Eraslan G, Saygi S, Essiz D, Aksoy A, Gul H, Macit E (2007) Evaluation of aspect of some oxidative stress parameters using vitamin E, proanthocyanidin and nacetylcysteine against exposure to cyfluthrin in mice. Pestic Biochem Physiol 88:43–49. CrossRefGoogle Scholar
  17. Habig WH, Pabst MJ, Jakoby WB (1974) Glutathione-S-transferases: the first enzymatic step in mercapturic acid formation. J Biol Chem 249:7130–7139Google Scholar
  18. Kalender S, Kalender Y, Ogutcu A, Uzunhisarcıklı M, Durak D, Acikgoz F (2004) Endosulfan-induced cardiotoxicity and free radical metabolism in rats: the protective effect of vitamin E. Toxicology 202:227–235. CrossRefGoogle Scholar
  19. Kalender S, Apaydin FG, Baş H, Kalender Y (2015) Protective effects of sodium selenite on lead nitrate-induced hepatotoxicity in diabetic and non-diabetic rats. Environ Toxicol Pharmacol 40:568–574. CrossRefGoogle Scholar
  20. Kim KS, Lim H, Lim JS, Son JY, Lee J, Lee BM, Chang SC, Kim HS (2018) Curcumin ameliorates cadmium-induced nephrotoxicity in Sprague-Dawley rats. Food Chem Toxicol 114:34–40. CrossRefGoogle Scholar
  21. Marklund S, Marklund G (1974) Involvement of the superoxide anion radical in the autoxidation of pyrogallol and a convenient assay for superoxide dismutase. Eur J Biochem 47:469–474. CrossRefGoogle Scholar
  22. Michalowicz J (2014) Bisphenol A—sources, toxicity and biotransformation. Environ Toxicol Pharmacol 27:738–758. CrossRefGoogle Scholar
  23. Ohkawa H, Ohishi N, Yagi K (1979) Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal Biochem 95:351–358. CrossRefGoogle Scholar
  24. Ortiz-Villanueva E, Navarro-Martín L, Jaumot J, Benavente F, Sanz-Nebot V, Pina B, Tauler R (2017) Metabolic disruption of zebrafish (Danio rerio) embryos by bisphenol A. An integrated metabolomics and transcriptomic approach. Environ Pollut 231:22–36. CrossRefGoogle Scholar
  25. Paglia DE, Valentine WN (1967) Studies on the quantative and qualitative characterization of erytrocyte glutathione peroxidase. J Lab Clin Med 70:158–169Google Scholar
  26. Poormoosavi SM, Najafzadehvarzi H, Behmanesh MA, Amirgholami R (2018) Protective effects of Asparagus officinalis extract against bisphenol A induced toxicity in Wistar rats. Toxicol Rep 5:427–433. CrossRefGoogle Scholar
  27. Quintana MM, Osimani VR, Magnarelli G, Rovedatti MG, Guiñazú N (2018) The insecticides chlorpyrifos and acetamiprid induce redox imbalance in umbilical cord blood erythrocytes in vitro. Pestic Biochem Physiol 148:87–92. CrossRefGoogle Scholar
  28. Richter CA, Birnbaum LS, Farabollini F, Newbold RR, Rubin BS, Talsness CE, Vandenberg JG, Walser-Kuntz DR, vom Saal FS (2007) In vivo effects of bisphenol A in laboratory rodent studies. Reprod Toxicol 24:199–224. CrossRefGoogle Scholar
  29. Sarkar P, Basak P, Ghosh S, Kundu M, Sil PC (2017) Prophylactic role of taurine and its derivatives against diabetes mellitus and its related complications. Food Chem Toxicol 110:109–121. CrossRefGoogle Scholar
  30. Sirdah MM (2015) Protective and therapeutic effectiveness of taurine in diabetes mellitus: a rationale for antioxidant supplementation. Diabetol Metab Syndr 9:55–64. CrossRefGoogle Scholar
  31. Soyalic H, Gevrek F, Karaman S (2017) Curcumin protects against acoustic trauma in the rat cochlea. Int J Pediatr Otorhinolaryngol 99:100–106. CrossRefGoogle Scholar
  32. Sreejayan N, Rao MNA (1994) Curcuminoids as potent inhibitors of lipid peroxidation. J Pharm Pharmacol 46:1013–1016. CrossRefGoogle Scholar
  33. Strimpakos AS, Sharma RA (2008) Curcumin: preventive and therapeutic properties in laboratory studies and clinical trials. Antioxid Redox Signal 10:511–545. CrossRefGoogle Scholar
  34. Takatani T, Takahashi K, Uozumi Y, Shikata E, Yamamoto Y, Ito T, Matsuda T, Schaffer SW, Fujio Y, Azuma J (2004) Taurine inhibits apoptosis by preventing formation of the Apaf-1/caspase-9 apoptosome. Am J Physiol Renal Physiol 287:C949–C953. CrossRefGoogle Scholar
  35. Tarapore P, Hennesy M, Son D, Ying J, Outang B, Govindarajah V, Leung Y, Ho S (2017) High butter-fat diet and bisphenol A additively impair male rats spermatogenesis. Reprod Toxicol 68:191–199. CrossRefGoogle Scholar
  36. Tzatzarakis MN, Vakonaki E, Kavvalakis MP, Barmpas M, Kokkinakis EN, Xenos K, Tsatsakis AM (2015) Biomonitoring of bisphenol A in hair of Greek population. Chemosphere 118:336–341. CrossRefGoogle Scholar
  37. Valokola MG, Karimi G, Razavi BM, Kianfar M, Jafarian AH, Jaafari MR, Imenshahidi M (2018) The protective activity of nanomicelle curcumin in bisphenol A-induced cardiotoxicity following subacute exposure in rats. Environ Toxicol 34:1–9. Google Scholar
  38. Zulli A (2011) Taurine in cardiovascular disease. Curr Opin Clin Nutr Metab Care 14:57–60. CrossRefGoogle Scholar

Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Faculty of Science, Department of BiologyGazi UniversityAnkaraTurkey
  2. 2.Gazi University- Vocational High School of Health ServicesAnkaraTurkey
  3. 3.Faculty of Arts and Science, Department of BiologyBozok UniversityYozgatTurkey
  4. 4.Faculty of Gazi Education, Department of ScienceGazi UniversityAnkaraTurkey

Personalised recommendations