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Polyphenolic extracts from the xerophyte Rhamnus lycioides as a radiation biodosimeter

  • Environmental Pollution, Food Contamination, Risk Assessment and Remediation
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Abstract

The majority of dosimeters currently in use are synthetic and very expensive. Therefore, the study of the dosimetric characteristics of polyphenolic extracts of xerophytes is useful because drought stress causes an increase in the production of these cheap and natural compounds containing benzene rings. Here, the polyphenolic compounds were extracted from Rhamnus lycioides which was collected from Bou-Hedma National Park in Tunisia and identified using liquid chromatography-mass spectrometry (LC-MS). We investigated the impact of cobalt-60 (60Co) irradiation (0–30 kilogray (kGy)) on the color parameters of polyphenolic extracts of R. lycioides using the Konica Minolta CR 300 portable colorimeter and UV–Visible spectroscopy. The structural and morphological characteristics of the irradiated extracts were assessed using Fourier transform infrared (FT-IR) spectroscopy, X-ray diffraction (XRD) technique and scanning electron microscopy (SEM). Overall, our results suggest that exposure to ionizing radiation (IR) of the polyphenolic components of the xerophyte R. lycioides has produced significant dose-dependent changes in their optical and morphological properties. Thus, these extracts can be valorized as biodosimeters in the dose range from 5 to 25 kGy.

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Acknowledgments

This work was supported by the Tunisian Ministry of Higher Education and Scientific Research; without this generous and strong assistance, this study could not have been undertaken. It was performed under the auspices of the Tunisian National Center for Nuclear Sciences and Technologies (CNSTN) in collaboration with the National Institute of Research and Physico-chemical Analyses of Tunisia (INRAP).

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Authors and Affiliations

  1. National Agronomic Institute of Tunisia (INAT), 43, Avenue Charles Nicolle, Mahrajène, 1082, Tunis, Tunisia

    Sihem Guesmi

  2. Laboratory “Energy and Matter for Development of Nuclear Sciences” (LR16CNSTN02), National Center for Nuclear Sciences and Technology, Sidi Thabet Technopark, 2020, Sidi Thabet, Tunisia

    Sihem Guesmi, Amel Raouafi, Faouzi Hosni & Haitham Sghaier

  3. Laboratoire d’Ecologie et d’Amélioration Sylvo-Pastorale, Institut National de Recherches en Genie Rural, Ariana, Tunisia

    Ismail Amri

  4. Laboratory of Useful Materials Valuation, National Center for Research in Materials Sciences, Borj Cedria Technopark, BP 73, 8027, Soliman, Tunisia

    Ahmed Hicham Hamzaoui

  5. Laboratory of Natural Substances (LR10INRAP02), National Institute of Research and Physico-chemical Analyses, Biotech Pole of Sidi Thabet, Ariana, Tunisia

    Abdennacer Boulila

  6. Faculty of Sciences, Bisha University, Bisha, Kingdom of Saudi Arabia

    Faouzi Hosni

  7. Associated with Laboratory “Biotechnology and Nuclear Technology” (LR16CNSTN01) and Laboratory “Biotechnology and Bio-Geo Resources Valorization” (LR11ES31), Sidi Thabet Technopark, 2020, Sidi Thabet, Tunisia

    Haitham Sghaier

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  1. Sihem Guesmi
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  2. Amel Raouafi
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  4. Ahmed Hicham Hamzaoui
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  5. Abdennacer Boulila
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  7. Haitham Sghaier
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Correspondence to Sihem Guesmi or Haitham Sghaier.

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Responsible editor: Philippe Garrigues

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Fig. S1

Post-irradiation stability of irradiated polyphenolic extracts at 25 kGy stored in dark at room temperature. (PNG 128 kb)

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Guesmi, S., Raouafi, A., Amri, I. et al. Polyphenolic extracts from the xerophyte Rhamnus lycioides as a radiation biodosimeter. Environ Sci Pollut Res 27, 5661–5669 (2020). https://doi.org/10.1007/s11356-018-3709-0

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