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Pesticide residues in Indian raw honeys, an indicator of environmental pollution

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Abstract

Honey has multifaceted beneficial properties, but polluted environment and unapproved apicultural practices have led to its contamination. In this study, QuEChERS method followed by chromatographic analysis by GC-μECD/FTD and GC-MS was validated and used for determination of 24 pesticides in 100 raw honey samples from various floral origins of Northern India. Matrix-matched calibrations showed that the method was selective and linear (r2 > 0.99) with detection limit < 9.1 ng g−1 for all the studied pesticides except for monocrotophos (21.3 ng g−1). The average recoveries at different fortification levels ranged from 86.0 to 107.7% with relative standard deviation < 20%. Pesticide residues were detected in 19.0% samples, and most prevalent compounds detected were dichlorvos in 6.0% samples followed by monocrotophos (5.0%), profenofos (5.0%), permethrin (4.0%), ethion (3.0%), and lindane (3.0%) with concentrations ranging from 58.8 to 225.5, from 96.0 to 430.1, from 14.6 to 43.2, from 27.8 to 39.6, from 25.6 to 28.0, and from 19.6 to 99.2 ng g−1, respectively. Honey samples originating from cotton, sunflower, and mustard crops (33.3%) that tested positive for pesticide residues were found to be significantly higher (p < 0.05) than the honey originating from natural and fruity vegetation (13.5%). Therefore, considering the contamination of environmental compartments due to extensive application of pesticides in the study area and their potential for subsequent transfer to honey by the expeditious bees, the results of present study proclaim that honey may be used as an indicator of environmental pollution. Further, estimated daily intakes of all contaminants were found to be at levels well below their acceptable daily intakes suggesting that consumption of honeys at current levels does not pose deleterious effects on human health. However, precautionary measures should always be taken considering the customary honey feeding in infants and cumulative effect of these chemicals in the foreseeable future.

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Acknowledgments

Authors are thankful to Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, Punjab (India) for providing the funds to carry out the present research.

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  1. School of Public Health and Zoonoses, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, Punjab, 141 004, India

    Atul Kumar, Jatinder Paul Singh Gill, Jasbir Singh Bedi & Amit Kumar

  2. Department of Veterinary Public Health and Epidemiology, CSK Himachal Pradesh Agricultural University, Palampur, Himachal Pradesh, 176062, India

    Atul Kumar

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Kumar, A., Gill, J.P.S., Bedi, J.S. et al. Pesticide residues in Indian raw honeys, an indicator of environmental pollution. Environ Sci Pollut Res 25, 34005–34016 (2018). https://doi.org/10.1007/s11356-018-3312-4

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