Environmental Science and Pollution Research

, Volume 26, Issue 14, pp 13825–13832 | Cite as

Analysis of methanogens adsorption and biogas production characteristics from different coal surfaces

  • Hongyu Guo
  • Zhiwei Dong
  • Xile Liu
  • Yang Bai
  • Zhixiang Gao
  • Daping XiaEmail author
Sustainable Environmental Management


The aim of this study was to examine the biogas production and the adsorption aspect of microorganism from different coals. Coal samples were obtained from Qianqiu mine and Guandi mine. Microbial populations were cultured from the coal mine drainage. After an anaerobic reaction period at about 35 °C, adsorption rate was determined by the spectrophotometer, while a scanning electron microscopy was used to observe the microorganisms on the coal and the headspace methane was analyzed using gas chromatography. Results show that the coal rank and particle size serve as important factors influencing the adsorption of microorganism and biogenic methane production. With decreasing particle size, the Qianqiu coal produced a considerable adsorption rate between 75 and 79%, while the adsorption rate of Guandi coal was between 52 and 74%. Meanwhile, the density of microorganisms from the Qianqiu coal surface demonstrated a higher level of adsorption than that of Guandi coal following the scanning electron microscopy images. Additionally, Qianqiu coal produced a higher level of biogas production (391.766–629.199 μmol/g) than that of Guandi coal (292.835–393.744 μmol/g) and the Qianqiu coal also generated a higher concentration of methane during the incubation. When the adsorption rate decreasing, the biogas production from various pulverized coals appeared to be decreased and demonstrated a positive correlation to the adsorption rate. The results of this study suggest that the adsorption behavior of microorganisms is closely related to the effect of coal biodegradation and contributes to the biogenic methane production potential.


Methanogens Adsorption rate Coal rank Biogas production Particle size 


Funding information

This study was funded by the National Science Foundation of China (Grant no. 41472127, 41472129, and 41502158), Program for Innovative Research Team in University of Ministry of Education of China (IRT_16R22), Shanxi Provincial Program for Tackling Key Problems of Coal-based Science and Technology (Grant no. MQ2014-01), Shanxi Province Joint Research Fund of Coalbed Methane (Grant no. 2013012004), and Scientific and Technological Research Projects of Henan Province (Grant no. 172102310717).


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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  • Hongyu Guo
    • 1
    • 2
    • 3
  • Zhiwei Dong
    • 1
  • Xile Liu
    • 1
  • Yang Bai
    • 1
  • Zhixiang Gao
    • 1
  • Daping Xia
    • 1
    • 3
    Email author
  1. 1.School of Energy Science and EngineeringHenan Polytechnic UniversityJiaozuoChina
  2. 2.State Key Laboratory Cultivation Base for Gas Geology and Gas ControlHenan Polytechnic UniversityJiaozuoChina
  3. 3.Collaborative Innovation Center of Coalbed Methane and Shale Gas for Central Plains Economic RegionJiaozuoChina

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