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Resistance of alfalfa and Indian mustard to Cd and the correlation of plant Cd uptake and soil Cd form

Environmental Science and Pollution Research volume 26, pages 13804–13811 (2019)Cite this article

Abstract

Phytoremediation of heavy metal-contaminated soil is considered to be one ecological environmental protection way that is effective and economical. The selection of suitable hyperaccumulators is a key issue for phytoremediation of heavy metal-contaminated soil. Pot experiments were conducted to study the effects of different Cd levels (0, 75, 150, 300, and 600 mg kg−1 Cd) on the dry weight, antioxidant enzyme activities, malondialdehyde (MDA) contents, Cd concentration, Cd accumulation, and soil Cd form distribution ratio (FDC) of alfalfa (Medicago sativa L.) and Indian mustard (Brassica juncea L.). The correlations between Cd concentration in shoots and roots of alfalfa and Indian mustard and soil Cd form were also investigated. The results showed that with the increase of soil Cd levels, dry weight of shoot and root of alfalfa and Indian mustard significantly decreased, which decreased by 50.0–71.8% and 29.6–59.3% (alfalfa), 59.6–89.0% and 64.3–74.8% (India mustard), respectively, compared with the control. With the increase of soil Cd level, superoxide dismutase (SOD) activity in shoot of alfalfa significantly increased. Catalase (CAT) activity and malondialdehyde contents in shoots and roots of alfalfa and Indian mustard, as well as superoxide dismutase activity in the roots of alfalfa and Indian mustard increased first and then decreased with the increase of soil Cd level. With increasing Cd stress, Cd concentration in shoots and roots of alfalfa and Indian mustard significantly increased. At soil Cd level of 75 mg kg−1, Cd concentrations in shoots of alfalfa and Indian mustard exceeded the critical value of Cd-hyperaccumulator (100 mg kg−1), which was 356.46 mg kg−1 and 308.74 mg kg−1, respectively. Cadmium concentrations in shoots and roots of plants were in the order of that of alfalfa > Indian mustard; total Cd accumulation in the aboveground tissues and roots of the plants was in the order of that of Indian mustard > alfalfa at the same Cd level. With increasing soil Cd level, Cd concentrations of exchangeable form (EXC-Cd), carbonate-bound form (CAB-Cd), iron-manganese oxide-bound form (FeMn-Cd), organic-bound form (OM-Cd), and residual form (RES-Cd) showed an increasing trend. The form distribution ratio of soil Cd forms in alfalfa’s rhizosphere was in the order of that of exchangeable form Cd > carbonate-bound form Cd > iron-manganese oxide-bound form Cd > residual form Cd > organic-bound form Cd. Except for organic-bound form Cd, soil Cd forms were significantly positively correlated with Cd concentration in shoot and root (P < 0.01). Comprehensively considering the biomass and Cd accumulation, Indian mustard is more suitable as remediation material for soil Cd pollution.

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Funding

This work was supported by Fund of China Agriculture Research System (CARS-23), the National Science and Technology Pillar Program of China (No. 2007BAD87B10), and the National key research and development program (2018YFD0201200).

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  1. College of Resources and Environmental Sciences, Southwest University, Chongqing, 400715, People’s Republic of China

    Chunlai Zhang, Yongqin Chen, Weihong Xu, Sunlin Chi, Tao Li, Yanhua Li, Zhangmi He, Mei Yang & Deyu Feng

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  1. Chunlai Zhang
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  2. Yongqin Chen
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Correspondence to Weihong Xu.

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Chunlai Zhang and Yongqin Chen should be as co-first authors.

Responsible editor: Philippe Garrigues

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Zhang, C., Chen, Y., Xu, W. et al. Resistance of alfalfa and Indian mustard to Cd and the correlation of plant Cd uptake and soil Cd form. Environ Sci Pollut Res 26, 13804–13811 (2019). https://doi.org/10.1007/s11356-018-3162-0

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  • DOI: https://doi.org/10.1007/s11356-018-3162-0

Keywords

  • Alfalfa
  • India mustard
  • Antioxidant enzyme activities
  • Plant Cd accumulation
  • Soil Cd form distribution