Environmental Science and Pollution Research

, Volume 25, Issue 31, pp 31497–31507 | Cite as

Utilization of freshwater mussel (Lamellidens marginalis) for the isolation of proteins through pH shift processing: characterization of isolates

  • Vijay Kumar Reddy SurasaniEmail author
  • Amit Mandal
  • Abhed Pandey
Research Article


Study was conducted to use underutilized freshwater mussel (Lamellidens marginalis) for the recovery of proteins using pH shift method and to study the functionality and characteristics of the recovered isolates. From the pH range tested (pH 2.0–13.0), maximum protein yields were obtained during solubilization at pH 2.0 and pH 13.0 (p < 0.05). During the protein recovery process, pH 13.0 was found to have minimal effect on proteins resulting in higher protein yields compared to pH 2.0. Isolates obtained by both acidic and alkaline solubilization processes had low stability and poor gel network. Total lipid content, total myoglobin, and pigment contents were reduced significantly (p < 0.05) during pH shift processing, resulting in whiter protein isolates and protein gels. All the essential amino acids were present in the isolates recovered by acid and alkaline solubilization, indicating the complete recovery of amino acids. No microbial counts were observed in any of the isolates prepared using acid and alkaline-aided processing. Acid and alkaline solubilization (pH shift) process was found to be promising for the recovery of proteins from underutilized freshwater mussel thus by reducing the supply demand gap.


Mussel Protein pH shift Acid Alkali Isolate 



Authors wish to express their sincere thanks to the Dean, College of Fisheries, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, India, for the support and facilities provided during the work period. Authors wish to express their sincere thanks to Mrs. Manvinder Kaur for her technical help during the manuscript preparation.


  1. Álvarez C, Lélu P, Lynch S, Tiwari BK (2017) Optimised protein recovery from mackerel whole fish by using sequential acid/alkaline isoelectric solubilization precipitation (ISP) extraction assisted by ultrasound. LWT Food Sci Technol 88:210–216. CrossRefGoogle Scholar
  2. AOAC (2000) Association of official analytical chemists, 16th edn. Washington, DCGoogle Scholar
  3. Batista I, Pires C, Nelhas R (2007) Extraction of sardine proteins by acidic and alkaline solubilisation. Food Sci Technol Int 13(3):189–194CrossRefGoogle Scholar
  4. Bidlingmeyer BA, Cohen SA, Tarvin TL (1984) Rapid analysis of amino acids using precolumn derivatisation. J Chromatogr 336:93–104CrossRefGoogle Scholar
  5. Chaijan M, Benjakul S, Visessanguan W, Faustman C (2006) Physicochemical properties, gel forming ability and myoglobin content of sardine (Sardinnella gibbosa) and mackerel (Rastrelliger kanagurta) surimi produced by conventional method and alkaline solubilization process. Eur Food Res Technol 222:58–63CrossRefGoogle Scholar
  6. Chaijan M, Undeland I (2015) Development of a new method for determination of total haem protein in fish muscle. Food Chem 173:1133–1141CrossRefGoogle Scholar
  7. Chen YC, Tou JC, Jaczynski J (2007) Protein recovery from rainbow trout (Oncorhynchus mykiss) processing byproducts via isoelectric solubilization/precipitation and its gelation properties as affected by functional additives. J Agric Food Chem 55(22):9079–9088CrossRefGoogle Scholar
  8. Cortes-Ruis J, Pachero-Aguilar R, Garcia-Sanchez G, Lugo-Sanches ME (2001) Functional characterization of a protein concentrate from bristly sardine made under acidic conditions. J Aquat Food Prod Technol 10:5–23CrossRefGoogle Scholar
  9. FAO (2009) Global agriculture towards 2050. High Level Expert Forum Rome, 12–13 October 2009Google Scholar
  10. FAO (2017) The future of food and agriculture; trends and challenges. RomeGoogle Scholar
  11. Fatin NS, Huda N, David W (2015) Physicochemical properties of Japanese scad (Decapterus Maruadsi) surimi prepared using the acid and alkaline solubilization methods. Int J Sci Eng Res 6(4):141–147Google Scholar
  12. Feng YM, Hultin HO (2001) Effect of pH on the rheological and structural properties of gels of water-washed chicken-breast muscle at physiological ionic strength. J Agric Food Chem 49:3927–3935CrossRefGoogle Scholar
  13. Freitas IR, Cortez-Wega WR, Prentice C (2015) Evaluation of properties of protein recovered from fish muscle by acid solubilization process. Int Food Res J 22(3):1067–1073Google Scholar
  14. Freitas IR, Gauterio GV, Rios DG, Prentice C (2011) Functionality of protein isolates from argentine anchovy (Engraulis anchoita) residue using pH shift processing. J Food Sci Eng 1:374–378Google Scholar
  15. Haldar A, Dey TK, Dhar P, Chakarabarti J (2014) Exploring the nutritive values of the fresh water mussel Lamellidens marginalis as potential functional food. IOSR J Env Sci Toxicol Food Technol 8(8):1–7CrossRefGoogle Scholar
  16. Hamm R (1994) The influence of pH on the protein net charge in the myofibrillar system. Rec Meat Conf Proc 47:5–9Google Scholar
  17. Harlow E, Lane D (1988) Antibodies: a laboratory manual. Cold Spring Harbor Laboratory Press, New YorkGoogle Scholar
  18. Huang L, Chen Y, Morrissey MT (1997) Coagulation of fish proteins from frozen fish mince wash water by ohmic heating. J Food Process Eng 20:285–300CrossRefGoogle Scholar
  19. Hultin HO, Kelleher SD (1999) Process of isolating a protein composition from a muscle source and protein composition. Patent US6005073Google Scholar
  20. Hultin HO, Kelleher SD (2000) High efficiency alkaline protein extraction. Patent US6136959Google Scholar
  21. Huss HH (1983) Fresk Fisk K valitet Og Holdbarhed. Fiskeriministeriets ForsφgslaboratoriumGoogle Scholar
  22. Jafarpour SA, Shabanpour B, Filabadi SS (2013) Biochemical properties of fish protein isolate (FPI) from silver carp (Hypophthalmychthis molitrix) by application of acid-alkali process compared to traditional prepared surimi. Ecopersia 1(3):315–327Google Scholar
  23. Jay JM (1986) Modern food microbiology. Van Nostrand Reinhold Company, New YorkGoogle Scholar
  24. Jongjareonrak A, Rawdkuen S, Chaijan M, Benjakul S, Osako K, Tanaka M (2010) Chemical compositions and characterization of skin gelatin from farmed giant catfish (Pangasianodon gigas). LWT Food Sci Technol 43:161–165CrossRefGoogle Scholar
  25. Kelleher SD, Hultin HO (2000) Functional chicken muscle protein isolates prepared using low ionic strength, acid solubilisation/precipitation. Rec Meat conf Proc 3:76–81Google Scholar
  26. Kinsella JE (1976) Functional properties of proteins in foods, a survey. CRC Crit Rev Food Sci Nutr 7:219–280CrossRefGoogle Scholar
  27. Kristinsson H, Demir N (2003) Functional fish protein ingredients from fish species of warm and temperate waters: comparison of acid- and alkali-aided processing vs. conventional surimi processing. In: Betchel P (ed) Advances in seafood byproducts 2002 conference proceedings. Alaska Sea Grant College program University of Alaska, pp. 277–295Google Scholar
  28. Kristinsson H, Ingadottir B (2006) Recovery and properties of muscle proteins extracted from tilapia (Oreochromis niloticus) light muscle by pH shift processing. J Food Sci 1(3):E132–E141CrossRefGoogle Scholar
  29. Kristinsson HG, Liang Y (2006) Effect of pH-shift processing and surimi processing on Atlantic croaker (Micropogonias undulates) muscle proteins. J Food Sci 71:C304–C312CrossRefGoogle Scholar
  30. Kristinsson H, Theodore AE, Demir N, Ingadottir B (2005) A comparative study between acid- and alkali-aided processing and surimi processing for the recovery of proteins from channel catfish muscle. J Food Sci 70(4):C298–C306CrossRefGoogle Scholar
  31. Kudo G, Okada M, Miyauchi D (1973) Gel-forming capacity of washed and unwashed flesh of some Pacific coast species of fish. Marine Fish Rev 32:10–15Google Scholar
  32. Laemmli UK (1970) Cleavage of structural proteins during assembly of the head of bacteriophage T4. Nature 227:680–685CrossRefGoogle Scholar
  33. Lubana GK, Kaur B, Surasani VKR (2016) Quality changes in fresh rohu (Labeo rohita) cutlets added with fibers from ragi, oat and jowar. Nutr Food Sci 46(4):571–582CrossRefGoogle Scholar
  34. Marmon SK, Undeland I (2010) Protein isolation from gutted herring (Clupea harengus) using pH-shift processes. J Agric Food Chem 58:10480–10486CrossRefGoogle Scholar
  35. Mutilangi WAM, Panyam D, Kilara A (1996) Functional properties of properties of hydrolysates from proteolysis of heat-denatured whey protein isolate. J Food Sci 61(2):270–274CrossRefGoogle Scholar
  36. Niki H, Kato T, Deya E, Igarashi S (1985) Recovery of protein from effluent of fish meat in producing surimi and utilization of recovered protein. Nippon Suisan Gakkaishi 51(6):959–964CrossRefGoogle Scholar
  37. Nolsøe H, Undeland I (2009) The acid and alkaline solubilization process for the isolation of muscle proteins: state of art. Food Bioprocess Technol 2:1–27CrossRefGoogle Scholar
  38. Nolsφe H, Marmon SK, Undeland I (2011) Application of filtration to recover solubilized proteins during ph-shift processing of blue whiting (Micromesistius poutassou); effects on protein yield and qualities of protein isolates. Open Food Sci J 5:1–9CrossRefGoogle Scholar
  39. Panpipat W, Chaijan M (2016) Biochemical and physicochemical characteristics of protein isolates from bigeye snapper (Priacanthus Tayenus) head by-product using pH shift method. Turk J Fish Aquat Sci 16:41–50Google Scholar
  40. Rawdkuen S, Sai-Ut S, Khamsorn S, Chaijan M, Benjakul S (2009) Biochemical and gelling properties of tilapia surimi and protein recovered using an acid-alkaline process. Food Chem 112:112–119CrossRefGoogle Scholar
  41. Reddy SVK (2016) Effect of formulation and processing methods on the quality and acceptability of cutlets made from minced meat of pangas (Pangasius pangasius). SAARC J Agric 14(1):25–36CrossRefGoogle Scholar
  42. Robinson HW, Hogden CG (1940) The biuret reaction in the determination of serum proteins. J Biol Chem 135:707–725Google Scholar
  43. Sathe SK, Deshpande SS, Salunkhe DK (1982) Functional properties of lupin seed (Supinus mutabilis) proteins and protein concentrates. J Food Sci 7:191–197Google Scholar
  44. Shabanpour B, Etemadian Y, Taghipour B (2015) Physicochemical and rheological parameters changes for determining the quality of surimi and kamaboko produced by conventional, acid and alkaline solubilization process methods from common kilka (Clupeonella cultriventris caspia). Iran J Fish Sci 14(4):826–845Google Scholar
  45. Surasani VKR (2017) Influence of rohu (Labeo rohita) deboning by-product on composition, physical properties and sensorial acceptability of rohu cutlets. Nutr Food Sci 47(3):398–408CrossRefGoogle Scholar
  46. Surasani VKR (2018) Acid and alkaline solubilization (pH shift) process: a better approach for the utilization of fish processing waste and by-products. Env Sci Pollut Res 25:18345–18363. CrossRefGoogle Scholar
  47. Surasani VKR, Khatkar SK, Singh S (2017a) Effect of process variables on solubility andrecovery yields of proteins from pangas (Pangasius pangasius) frames obtained by alkalinesolubilization method: characteristics of isolates. Food Bioprod Process 106:137–146CrossRefGoogle Scholar
  48. Surasani VKR, Tyagi A, Kudre T (2017b) Recovery of proteins from rohu processing waste using ph shift method: characterization of isolates. J Aquat Food Prod Technol 26(3):356–365CrossRefGoogle Scholar
  49. Surasani VKR, Kudre T, Ballari RV (2018) Recovery and characterization of proteins from pangas (Pangasius pangasius) processing waste obtained through pH shift processing. Env Sci Pollut Res 25:11987–11998. CrossRefGoogle Scholar
  50. Tian Y, Wang W, Yuan C, Zhang L, Liu J, Liu J (2016) Nutritional and digestive properties of protein isolates extracted from the muscle of the common carp using pH shift processing. J Food Process Preserv 41:e12847. CrossRefGoogle Scholar
  51. Undeland I, Kelleher SD, Hultin HO (2002) Recovery of functional proteins from herring (Clupea harengus) light muscle by an acid or alkaline solubilization process. J Agric Food Chem 50(25):7371–7379CrossRefGoogle Scholar
  52. Vareltzis PK, Undeland I (2012) Protein isolation from blue mussels (Mytilus edulis) using an acid and alkaline solubilisation technique—process characteristics and functionality of the isolates. J Sci Food Agric 92(15):3055–3064CrossRefGoogle Scholar
  53. Yusufzi SI, Singh H, Shirdhankar MM (2010) An evaluation of different methods for transportation of the freshwater mussel Lamellidens corrianus (Lea 1834). Int J Aqua 18(4):676–692Google Scholar

Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Department of Harvest and Post-harvest Technology, College of FisheriesGuru Angad Dev Veterinary and Animal Sciences UniversityLudhianaIndia
  2. 2.Department of Aquaculture, College of FisheriesGuru Angad Dev Veterinary and Animal Sciences UniversityLudhianaIndia

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