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Differential transcriptional changes in human alveolar epithelial A549 cells exposed to airborne PM2.5 collected from Shanghai, China

Environmental Science and Pollution Research volume 25, pages 33656–33666 (2018)Cite this article

Abstract

Fine particulate matters (PM2.5) are the core pollutants of haze episode, which pose a serious threat to the human health of developing countries. However, the mechanisms involved in PM2.5-induced hazard influence are not to fully elucidated. In the present study, human lung epithelial cells (A549) were exposed to various concentrations of PM2.5 samples collected from Shanghai, China. Illumina RNA-Seq method with transcriptome, Gene Ontology (GO), and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis were further employed to identify the detrimental effects of PM2.5 on A549 cells in vitro. A total of 712 differentially expressed genes were obtained from global transcriptome profiling of A549 cells after PM2.5 exposure. In addition, GO function enrichment analysis revealed that major differentially expressed genes (DEGs) involved in the biological process of the immune system and the response to the stress. KEGG pathway analysis further proposes that infectious disease, cancers, cardiovascular disease, and immune disease pathway were the key human disease events that occur in A549 cells under PM2.5 stress. The data obtained here shed light on the related biological process and gene signaling pathways affected by PM2.5 exposure. This study aids our understanding of the complicated mechanisms related to PM2.5-induced health effects and is informative for the prevention and treatment of PM2.5-induced systemic diseases.

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Acknowledgments

Dr. Ping Wang (School of Biotechnology, ECUST) kindly provided the A549 cells. Shanghai Personal Biotechnology Limited Company provided RNA-Seq testing services.

Funding

This work was jointly supported by the Shanghai Pujiang Program (13PJD013), the Ph.D. Programs Foundation of Ministry of Education of China (No. 20120074140001), and the Fundamental Research Funds for the Central Universities (WB1113005). The author Xiaoning Lei gratefully acknowledge the financial support from the China Scholorship Council (CSC[2016]8248).

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Affiliations

  1. State Environmental Protection Key Laboratory of Risk Assessment and Control on Chemical Processes, East China University of Science and Technology (ECUST), Shanghai, 200237, China

    Xiaoning Lei, Zhongsi Huang, Guangli Xiu & Jiahui Chen

  2. Department of Public Health Sciences, The Pennsylvania State University College of Medicine, Penn State Hershey Medical Center, Hershey, PA, 17033, USA

    Xiaoning Lei & Joshua E. Muscat

  3. State Key Laboratory of Bioreactor Engineering, Biomedical Nanotechnology Center, School of Biotechnology, East China University of Science and Technology (ECUST), Shanghai, 200237, China

    Chao Chen

  4. Shanghai Environmental Protection Key Laboratory for Environmental Standard and Risk Management of Chemical Pollutants, East China University of Science and Technology (ECUST), Shanghai, 200237, China

    Guangli Xiu

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  1. Xiaoning Lei
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  2. Joshua E. Muscat
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  3. Zhongsi Huang
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  4. Chao Chen
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  5. Guangli Xiu
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Correspondence to Guangli Xiu.

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Lei, X., Muscat, J.E., Huang, Z. et al. Differential transcriptional changes in human alveolar epithelial A549 cells exposed to airborne PM2.5 collected from Shanghai, China. Environ Sci Pollut Res 25, 33656–33666 (2018). https://doi.org/10.1007/s11356-018-3090-z

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  • DOI: https://doi.org/10.1007/s11356-018-3090-z

Keywords

  • PM2.5
  • A549
  • Cytotoxicity
  • Differentially expressed genes
  • Transcriptome analysis