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Environmental Science and Pollution Research

, Volume 26, Issue 14, pp 13785–13794 | Cite as

Diversity in flower colorations of Ranunculus asiaticus L. revealed by anthocyanin biosynthesis pathway in view of gene composition, gene expression patterns, and color phenotype

  • Yanfang Liu
  • Jianhua Zhang
  • Xiaohong Yang
  • Jiangmin Wang
  • Yangang Li
  • Peng Zhang
  • Jin Mao
  • Qingmei HuangEmail author
  • Hao TangEmail author
Sustainable Environmental Management

Abstract

Anthocyanin biosynthesis is one of the best studied secondary metabolisms. However, related pathways were generally concluded based on anthocyanin components; most studies focused on the backbone forming of anthocyanidins (cyanidin, delphinidin, and pelargonidin) of model or commercial plants, while anthocyanin modification was less discussed, and non-model plants with abundant colorations were less researched either. Ranunculus asiaticus L. has great diversity in flower colorations, not only indicating its value in researching anthocyanin biosynthesis but also implying it is unique in this regard. Based on transcriptome sequencing and gene annotation of three varieties (10 samples) of Ranunculus asiaticus L., 176 unigenes from 151,136 unigenes were identified as involved in anthocyanin biosynthesis, among which, 74 unigenes were related to anthocyanin modification; 61 unigenes were responsible for glycosylation at C3 and C5 with 3-monosaccharides of glucose, 3-biosides of rutinose, sophorose, or sambubiose to form 3Gly-, 3Gly5Gly-, 3Gly3′Gly-, 3Gly2′′Gly-, 3Gly2′′Xly-, 3Gly2′′Rly-glycosylated anthocyanins, etc.; 2 unigenes transferred –CH3; 11 unigenes of BAHD family catalyzd the aromatic or malonyl acylation at 6′′ / 6′′′′position of 3/5-O-glucoside. Based on gene composition, a putative pathway was established. The pathway was validated by flower colorations, and gene expression patterns where F3H, F3H, 3GT, 5GT, and FMT2 were highly expressed in varieties colored as lateritious and carmine, while variety with purple flowers had high expression of F35H and 3MAT. In view of anthocyanin biosynthesis pathway of Ranunculus asiaticus L., great diversity in its flower colorations was illustrated via the complete branches (F3H, F3′H and F3′5′H) as well as complete modifications (glycosylation, methylation, and acylation), and besides, via the higher percentage of C3 glycosylation than C5 glycosylation.

Keywords

Ranunculus asiaticus L. Anthocyanin biosynthesis Gene composition Gene expression Flower colorations 

Notes

Authors’ contributions

Y.L. and H.T. conceived and designed the experiments; Y.L., J.Z., J.W., and Q.H. performed the experiments; Y.L., X.Y., and P.Z. analyzed the data; Y.L. and J.M. collected data; Y.L. and Q.H. wrote the paper.

Funding

This work was supported by grants from the National Natural Science Foundation of China (CN) (31760589), Natural Science Foundation of Yunnan Province (2015FB158), Joint Special Project of Yunnan Province for Agricultural Basic Research (2017FG01 (−032)), Personnel Training Project of Yunnan Province (2015HB100), and Protection of Plant Variety Resources of Ministry of Agriculture (1120162130135396009).

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  • Yanfang Liu
    • 1
  • Jianhua Zhang
    • 1
  • Xiaohong Yang
    • 1
  • Jiangmin Wang
    • 1
  • Yangang Li
    • 1
  • Peng Zhang
    • 1
  • Jin Mao
    • 1
  • Qingmei Huang
    • 1
    Email author
  • Hao Tang
    • 2
    Email author
  1. 1.DUS Test (Kunming) Station of Ministry of Agriculture, Quality Standard and Testing Technology Research InstituteYunnan Academy of Agricultural SciencesKunmingPeople’s Republic of China
  2. 2.Development Center of Science and TechnologyMinistry of AgricultureBeijingPeople’s Republic of China

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