Reduced arsenic availability and plant uptake and improved soil microbial diversity through combined addition of ferrihydrite and Trichoderma asperellum SM-12F1

Abstract

Arsenic (As) accumulation in agricultural soils is prone to crop uptake, posing risk to human health. Passivation shows potential to inactivate soil labile As and lower crop As uptake but often contributes little to improving the microbiota in As-contaminated soils. Here, the combined addition of ferrihydrite and Trichoderma asperellum SM-12F1 as a potential future application for remediation of As-contaminated soil was studied via pot experiments. The results indicated that, compared with the control treatment, the combined addition of ferrihydrite and T. asperellum SM-12F1 significantly increased water spinach shoot and root biomass by 134 and 138%, respectively, and lowered As content in shoot and root by 37 and 34%, respectively. Soil available As decreased by 40% after the combined addition. The variances in soil pH and As fractionation and speciation were responsible for the changes in soil As availability. Importantly, the combined addition greatly increased the total phospholipid fatty acids (PLFAs) and gram-positive (G+), gram-negative (G−), actinobacterial, bacterial, fungal PLFAs by 114, 68, 276, 292, 133, and 626%, respectively, compared with the control treatment. Correspondingly, the soil enzyme activities closely associated with carbon, nitrogen, and phosphorus mineralization and antioxidant activity were improved. The combination of ferrihydrite and T. asperellum SM-12F1 in soils did not reduce their independent effects.

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Funding

This study was financially supported by the National Foundation of Natural Science of China, Grant No. 41671328 and the Yong Elite Scientist Sponsorship Program by the China Association for Science and Technology, Grant No.2015QNRC001.

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Correspondence to Shiming Su.

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Core ideas

• Ferrihydrite (FH) and T. asperellum (CH) were applied into soil As remediation

• FH + CH improved water spinach growth and reducing As uptake

• FH + CH addition significantly decreased soil available As content by 40%

• FH + CH addition improved soil microbial diversity and enzyme activities

Responsible editor: Robert Duran

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Zhang, H., Zeng, X., Bai, L. et al. Reduced arsenic availability and plant uptake and improved soil microbial diversity through combined addition of ferrihydrite and Trichoderma asperellum SM-12F1. Environ Sci Pollut Res 25, 24125–24134 (2018). https://doi.org/10.1007/s11356-018-2451-y

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Keywords

  • Ferrihydrite
  • Arsenic
  • Fungi
  • PLFA
  • Water spinach
  • Availability