Environmental Science and Pollution Research

, Volume 25, Issue 18, pp 17278–17282 | Cite as

Biological control of Meloidogyne javanica on tomato with Dazitol® and soil solarization

  • Lobna Hajji-Hedfi
  • Emna Rebai
  • Asma Larayedh
  • Hajer Regaieg
  • Najet Horrigue-Raouani
Research Article


Pot and greenhouse trials were conducted for the management of root-knot nematode, Meloidogyne javanica, infestation in tomato. Growth parameters, gall index, soil, and root nematode populations were measured to assess the effect of a novel bio-pesticide (Dazitol®), made from mustard oil and oleoresin of Capsicum, on plant growth and nematode reproduction. Data generated within the pot experiment showed that the tested bio-pesticide did not improve plant growth, but it reduced significantly root-knot nematode damage resulting in a decrease in gall index and root (91%) and soil (62%) population of M. javanica compared with untreated plants. The greenhouse experiment showed that Mocap® and Dazitol® decreased nematode incidence significantly (P < 0.05) on tomato. The result of this study suggested that the best nematode control was obtained by combining soil solarization with chemical or botanical nematicides as an integrated pest management approach.


Biopesticides Tomato Root-knot nematode Solarization Pest management 


Compliance with ethical standards

This study does not involve any human and/or animal participant.

Conflict of interest

The authors declare that they have no conflict of interest.


  1. Abd-Elgawad MM (2008) The current status of phytonematode management in Egypt with special reference to applicable nematicides. Egypt J Agron 6:33–46Google Scholar
  2. Caboni P, Ntalli NG (2014) Botanical nematicides, recent findings. In: Gross AD, Coats JR, Duke SO, Seiber JN (eds) Biopesticides: state of the art and future opportunities. American Chemical Society, Washington DC, pp 145–157Google Scholar
  3. Coolen WA (1979) Methods for the extraction of Meloidogyne spp. & other nematodes from roots and soil. In: Lamberti F, Taylor CE (eds) Root-knot nematodes (Meloidogyne Species) systematics, biology and control. Academic Press, London, pp 317–329Google Scholar
  4. De Grisse AT (1969) Redescription ou modification de quelques techniques utilisée dans l’étude des nématodes phytoparasitaires. Mededelingen Rijksfaculteit der Landbouwetinschap, Gent, pp 351–369Google Scholar
  5. Elhajji L, Horrigue-Raouani N (2012) Efficiency of solarization and incorporation of cattle manure in management of Meloidogyne javanica (Treub.) Chitwood and Globodera pallida (stone) Behrens associated with potato. Pest Technol 6:75–78Google Scholar
  6. Fleming CC, Turner SJ, Hunt M (2006) Management of root knot nematodes in turfgrass using mustard formulations and biostimulants. Commun Agric Appl Biol Sci 71(3):653–658Google Scholar
  7. Gamliel A, Stapleton JJ (1993) Characterization of antifungal volatile compounds evolved from solarized soil amended with cabbage residues. Phytopathology 83:899–905CrossRefGoogle Scholar
  8. Horrigue-Raouani N (2003) Variabilité de la relation hôte parasite dans le cas des Meloidogyne spp. (Nematoda: Meloidogynidae). Thèse de docteur d’état. Université Tunis-El Manar, Faculté des Sciences de Tunis, Tunis, pp 222Google Scholar
  9. Hussey RS, Barker KR (1973) A comparison of methods of collecting inocula of Meloidogyne spp. including a new technique. Plant Dis Rep 57:1025–1028Google Scholar
  10. Hussey RS, Janssen GJW (2002) Root-knot nematode: Meloidogyne species. In: Starr JL, Cook R, Bridge J (eds) Plant resistance to parasitic nematodes. CAB International, Wallingford, pp 43–70CrossRefGoogle Scholar
  11. Katan J, Greenberger A, Alon H, Grinstein A (1976) Solar heating by polyethylene mulching for the control of diseases caused by soilborne pathogens. Phytopathology 66:683–688CrossRefGoogle Scholar
  12. Martin TJ, Turner SJ, Fleming CC (2007) Management of the potato cyst nematode (Globodera pallida) with bio-fumigants/stimulants. Commun Agric Appl Biol Sci 72(3):671–675Google Scholar
  13. Mekete T, Decraemer W, Wesemael WML, Seid A, Fininsa C (2015) Tomato (Solanum lycopersicum) and root-knot nematodes (Meloidogyne spp.)—a century-old battle. Nematology 17:1–15CrossRefGoogle Scholar
  14. Moens M, Perry RN, Starr JL (2009) Meloidogyne species—a diverse group of novel and important plant parasites. In: Perry RN, Moens M, Starr JL (eds) Root knot nematodes. CAB International, Wallingford, p 483Google Scholar
  15. Oka Y, Nurit S, Pinchas F (2007) Control of root-knot nematodes in organic farming systems by organic amendments and soil solarization. Crop Prot 26(10):1556–1565CrossRefGoogle Scholar
  16. Pharand B, Carisse O, Benhamou N (2002) Cytological aspects of compost-mediated induced resistance against Fusarium crown and root rot in tomato. Phytopathology 92:424–438CrossRefGoogle Scholar
  17. Ploeg A (2008) Biofumigation to manage plant-parasitic nematodes. In: Ciancio A, Mukherjee KG (eds) Integrated management and biocontrol of vegetable and grain crop nematodes. Springer, Dordrecht, pp 239–248Google Scholar
  18. Reddy PP (2011) Biofumigation and solarization for management of soil borne plant pathogens, vol 431. Scientific publishers, JodhpurGoogle Scholar
  19. Salem MF (2014) Innovative approaches through modified biofumigation in controlling soil-borne pathogens and root-knot nematode. Aspects of Applied Biology 126, 5th International Symposium of Biofumigation, UKGoogle Scholar
  20. Seid A, Fininsa C, Mekete T, Decraemer W, Wesemael W (2015) Tomato (Solanum lycopersicum) and root-knot nematodes (Meloidogyne spp.): a century-old battle. Nematology 17(9):995–1009CrossRefGoogle Scholar
  21. Sikora RA, Fernandez E (2005) Nematode parasites of vegetables. In: Luc M, Sikora RA, Bridge J (eds) Plant parasitic nematodes in subtropical and tropical agriculture. CAB International, Wallingford, pp 319–392CrossRefGoogle Scholar
  22. Stapleton JJ, Heald CM (1991) Management of phytoparasitic nematodes by soil solarization. In: Katan J, JE DV (eds) Soil solarization. CRC Press, Boca Raton, pp 51–59Google Scholar
  23. Stoddard FL, Nicholas AH, Rubiales D, Thomas J, Villegas-Fernández AM (2010) Integrated pest management in faba bean. Field Crop Res 115(3):308–318CrossRefGoogle Scholar
  24. Talavera M, Sayadi S, Chirosa-Ríos M, Salmerón T, Flor-Peregrín E, Verdejo-Lucas S (2012) Perception of the impact of root-knot nematode-induced diseases in horticultural protected crops of South-Eastern Spain. Nematology 14:517–527CrossRefGoogle Scholar
  25. Thoden TC, Korthals GW, Termorshuizen AJ (2011) Organic amendments and their influence on plant parasitic and free living nematodes: a promising method for nematode management. Nematology 13:133–153CrossRefGoogle Scholar

Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Department of Biological Sciences and Plant Protection, Higher Agronomic Institute of Chott-MariemSousse UniversitySousseTunisia

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