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Interactions between tetrahydroisoindoline-1,3-dione derivatives and human serum albumin via multiple spectroscopy techniques

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Abstract

Some tetrahydroisoindoline-1,3-dione derivatives (TDDs) possess potent herbicidal activity. To assess possible impacts of TDDs on humans, the interactions between TDDs and human serum albumin (HSA) were evaluated with steady-state and time-resolved fluorescence spectroscopy, synchronous fluorescence spectroscopy, Fourier transform-infrared spectroscopy, and circular dichroism spectroscopy. The thermodynamic data obtained at temperatures of 298, 307, and 316 K indicate that TDDs spontaneously bind to HSA and thus form a TDD-HSA complex. The conformation and secondary structure of HSA are changed, and the intrinsic fluorescence of HSA is statically quenched by TDDs. Moreover, the TDD-HSA complex is formed primarily through electrostatic interactions and has only one binding site on HSA. A competitive ligand-binding assay revealed that site II (subdomain IIIA) displays the greatest affinity for TDDs. In addition, an acute toxicity bioassay showed no zebrafish mortality upon exposure to 4000 μg L−1 of TDDs. This work is helpful for understanding interactions between TDDs and HSA.

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Funding

The authors gratefully acknowledge the financial support from the National Natural Science Foundation of China (No. 31601657), the Anhui Agricultural University Youth Fund Project (2014zr003 and yj2015-25), the Provincial Training Programs of Innovation and Entrepreneurship for Undergraduates (No. 201610364024), Open Funding of Key Laboratory of Agri-food Safety of Anhui Province, Open Funding of State Key Laboratory of Tea Plant Biology and Utilization, and Open Funding of State Key Laboratory of Elemento-organic Chemistry.

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Authors and Affiliations

  1. Key Laboratory of Agri-food Safety of Anhui Province, College of Resources and Environment, Anhui Agricultural University, No. 130 Changjiang West Road, Hefei, 230036, China

    Meiqing Zhu, Lijun Wang, Shisuo Fan, Zhen Wang & Yi Wang

  2. Department of Applied Chemistry, College of Science, China Agricultural University, No. 2 Yuanmingyuan West Road, Beijing, 100193, China

    Hao Zhang, Yi Wang & Shangzhong Liu

  3. Department of Molecular Biosciences and Bioengineering, University of Hawaii at Manoa, Honolulu, HI, 96822, USA

    Qing X. Li

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  1. Meiqing Zhu
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  2. Lijun Wang
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  3. Hao Zhang
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  4. Shisuo Fan
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  5. Zhen Wang
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  6. Qing X. Li
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  7. Yi Wang
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  8. Shangzhong Liu
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Correspondence to Yi Wang or Shangzhong Liu.

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Zhu, M., Wang, L., Zhang, H. et al. Interactions between tetrahydroisoindoline-1,3-dione derivatives and human serum albumin via multiple spectroscopy techniques. Environ Sci Pollut Res 25, 17735–17748 (2018). https://doi.org/10.1007/s11356-018-1955-9

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