Environmental Science and Pollution Research

, Volume 25, Issue 18, pp 17645–17653 | Cite as

Characterization of a di-n-butyl phthalate-degrading bacterial consortium and its application in contaminated soil

  • Jing Yang
  • Chuling Guo
  • Shasha Liu
  • Weiting Liu
  • Han Wang
  • Zhi Dang
  • Guining Lu
Research Article


Dibutyl phthalate (DBP), as a plasticizer, is widely used in China, and it is easily released into diverse environments. In this study, we have obtained a stable bacterial consortium (B1) enriched from municipal sewage treatment plant activated sludge. The obtained bacterial consortium B1 was capable of degrading DBP and was mainly composed of Pandoraea sp. and Microbacterium sp. From the initial concentrations of 35–500 mg L−1, DBP was efficiently degraded by the consortium, with the degradation rates above 92% within 3 days. The optimal temperature for DBP degradation was 30 °C and consortium B1 could adapt to a wide range of pH (5.5–8.5). The analysis of Illumina sequencing further showed that the relative abundance of Pandoraea was increased at the beginning of the degradation, while Microbacterium was decreased. In the later stage of the degradation, the change of the relative abundance of Pandoraea and Microbacterium was opposite. Apart from DBP, consortium B1 could also utilize dimethyl phthalate (DMP), di-2-ethylhexyl phthalate (DEHP), and phthalic acid (PA) as the sole carbon. Moreover, adding B1 to DBP-contaminated soil could greatly improve the removal rate of DBP, suggesting that B1 has a great potential for the bioremediation of DBP-contaminated environments.


Di-n-butyl phthalate Bacterial consortium Biodegradation Degradation pathway Illumina sequencing Soil bioremediation 


Funding information

This work was supported by the Guangdong Provincial Science and Technology Projects (2014A020217002 and 2016B020242004).

Supplementary material

11356_2018_1862_MOESM1_ESM.pdf (365 kb)
ESM 1 (PDF 343 kb)


  1. Ascon-Cabrera M, Lebeault JM (1993) Selection of xenobiotic-degrading microorganisms in a biphasic aqueous-organic system. Appl Environ Microbiol 59:1717–1724Google Scholar
  2. Caporaso JG, Lauber CL, Walters WA, Berg-Lyons D, Huntley J, Fierer N, Owens SM, Betely J, Fraser L, Bauer M, Gormley N, Gilbert JA, Smith G, Knight R (2012) Ultra-high-throughput microbial community analysis on the Illumina HiSeq and MiSeq platforms. ISME J 6:1621–1624CrossRefGoogle Scholar
  3. Chang BV, Wang TH, Yuan SY (2007) Biodegradation of four phthalate esters in sludge. Chemosphere 69:1116–1123CrossRefGoogle Scholar
  4. Chen JA, Li X, Li J, Cao J, Qiu Z, Zhao Q, Xu C, Shu W (2007) Degradation of environmental endocrine disruptor di-2-ethylhexyl phthalate by a newly discovered bacterium, Microbacterium sp. strain CQ0110Y. Appl Microbiol Biotechnol 74:676–682CrossRefGoogle Scholar
  5. Chen S, Geng P, Xiao Y, Hu M (2012) Bioremediation of β-cypermethrin and 3-phenoxybenzaldehyde contaminated soils using Streptomyces aureus HP-S-01. Appl Microbiol Biotechnol 94:505–515CrossRefGoogle Scholar
  6. Chen X, Zhang XL, Yang Y, Yue DM, Xiao L, Yang LY (2015) Biodegradation of an endocrine-disrupting chemical di-n-butyl phthalate by newly isolated Camelimonas sp. and enzymatic properties of its hydrolase. Biodegradation 26:171–182CrossRefGoogle Scholar
  7. Colbert CL, Agar NY, Kumar P, Chakko MN, Sinha SC, Powlowski JB, Eltis LD, Bolin JT (2013) Structural characterization of Pandoraea pnomenusa B-356 biphenyl dioxygenase reveals features of potent polychlorinated biphenyl-degrading enzymes. PLoS One 8:e52550CrossRefGoogle Scholar
  8. Fang HH, Liang D, Zhang T (2007) Aerobic degradation of diethyl phthalate by Sphingomonas sp. Bioresour Technol 98:717–720CrossRefGoogle Scholar
  9. Fang CR, Yao J, Zheng YG, Jiang CJ, Hu LF, Wu YY, Shen DS (2010) Dibutyl phthalate degradation by Enterobacter sp. T5 isolated from municipal solid waste in landfill bioreactor. Int Biodeterior Biodegrad 64:442–446CrossRefGoogle Scholar
  10. Fredricsson B, Moller L, Pousette A, Westerholm R (1993) Human sperm motility is affected by plasticizers and diesel particle extracts. Basic Clin Pharmacol Toxicol 72:128–133CrossRefGoogle Scholar
  11. He Z, Xiao H, Tang L, Min H, Lu Z (2013) Biodegradation of di-n-butyl phthalate by a stable bacterial consortium, HD-1, enriched from activated sludge. Bioresour Technol 128:526–532CrossRefGoogle Scholar
  12. He L, Gielen G, Bolan NS, Zhang X, Qin H, Huang H, Wang H (2015) Contamination and remediation of phthalic acid esters in agricultural soils in China: a review. Agron Sustain Dev 35:519–534CrossRefGoogle Scholar
  13. Hudcova T, Halecky M, Kozliak E, Stiborova M, Paca J (2011) Aerobic degradation of 2,4-dinitrotoluene by individual bacterial strains and defined mixed population in submerged cultures. J Hazard Mater 192:605–613CrossRefGoogle Scholar
  14. Jin DC, Liang RX, Dai QY, Zhang RY, Wu XL, Chao WL (2010) Biodegradation of di-n-butyl phthalate by Rhodococcus sp. JDC-11 and molecular detection of 3, 4-phthalate dioxygenase gene. J Microbiol Biotechnol 20:1440–1445CrossRefGoogle Scholar
  15. Jin DC, Bai ZH, Chang DD, Hoefel D, Jin B, Wang P, Wei DB, Zhuang GQ (2012) Biodegradation of di-n-butyl phthalate by an isolated Gordonia sp. strain QH-11: genetic identification and degradation kinetics. J Hazard Mater 221:80–85CrossRefGoogle Scholar
  16. Jin DC, Kong X, Cui BJ, Bai ZH, Zhang HX (2013) Biodegradation of di-n-butyl phthalate by a newly isolated halotolerant Sphingobium sp. Int J Mol Sci 14:24046–24054CrossRefGoogle Scholar
  17. Li JX, Gu JD, Pan L (2005) Transformation of dimethyl phthalate, dimethyl isophthalate and dimethyl terephthalate by Rhodococcus rubber Sa and modeling the processes using the modified Gompertz model. Int Biodeterior Biodegrad 55:223–232CrossRefGoogle Scholar
  18. Li C, Tian X, Chen Z, Yu D, Deng J, Xu H (2012) Biodegradation of an endocrine-disrupting chemical di-n-butyl phthalate by Serratia marcescens C9 isolated from activated sludge. Afr J Microbiol Res 6:2686–2693Google Scholar
  19. Li J, Wang G, Aggarwal SG, Huang Y, Ren Y, Zhou B, Singh K, Gupta PK, Cao J, Zhang R (2014) Comparison of abundances, compositions and sources of elements, inorganic ions and organic compounds in atmospheric aerosols from Xi'an and New Delhi, two megacities in China and India. Sci Total Environ 476:485–495CrossRefGoogle Scholar
  20. Liao CS, Chen LC, Chen BS, Lin SH (2010) Bioremediation of endocrine disruptor di-n-butyl phthalate ester by Deinococcus radiodurans and Pseudomonas stutzeri. Chemosphere 78:342–346CrossRefGoogle Scholar
  21. Liang DW, Zhang T, Fang HHP, He J (2008) Phthalates biodegradation in the environment. Appl Microbiol Biotechnol 80:183–198CrossRefGoogle Scholar
  22. Matsumoto M, Hirata KM, Ema M (2008) Potential adverse effects of phthalic acid esters on human health: a review of recent studies on reproduction. Regul Toxicol Pharmacol 50:37–49CrossRefGoogle Scholar
  23. Nahurira R, Ren L, Song J, Jia Y, Wang J, Fan S, Wang H, Yan Y (2017) Degradation of di (2-Ethylhexyl) phthalate by a novel Gordonia alkanivorans strain YC-RL2. Curr Microbiol 74:309–319CrossRefGoogle Scholar
  24. Net S, Rabodonirina S, Sghaier RB, Dumoulin D, Chbib C, Tlili I, Ouddane B (2015) Distribution of phthalates, pesticides and drug residues in the dissolved, particulate and sedimentary phases from transboundary rivers (France–Belgium). Sci Total Environ 521:152–159CrossRefGoogle Scholar
  25. Okeke BC, Siddique T, Arbestain MC, Frankenberger WT (2002) Biodegradation of γ-hexachlorocyclohexane (lindane) and α-hexachlorocyclohexane in water and a soil slurry by a Pandoraea species. J Agr Food Chem 50:2548–2555CrossRefGoogle Scholar
  26. Prasad B, Suresh S (2012) Biodegradation of dimethyl phthalate, diethyl phthalate, dibutyl phthalate and their mixture by Variovorax sp. Int J Environ Sci Dev 3:283–288CrossRefGoogle Scholar
  27. Ren L, Jia Y, Ruth N, Qiao C, Wang J, Zhao B, Yan Y (2016) Biodegradation of phthalic acid esters by a newly isolated Mycobacterium sp. YC-RL4 and the bioprocess with environmental samples. Environ Sci Pollut Res 23:16609–16619CrossRefGoogle Scholar
  28. Saratale RG, Saratale GD, Kalyani DC, Chang JS, Govindwar SP (2009) Enhanced decolorization and biodegradation of textile azo dye Scarlet R by using developed microbial consortium-GR. Bioresour Technol 100:2493–2500CrossRefGoogle Scholar
  29. Selvaraj KK, Sundaramoorthy G, Ravichandran PK, Girijan GK, Sampath S, Ramaswamy BR (2015) Phthalate esters in water and sediments of the Kaveri River, India: environmental levels and ecotoxicological evaluations. Environ Geochem Health 37:83–96CrossRefGoogle Scholar
  30. Staples CA, Peterson DR, Parkerton TF, Adams WJ (1997) The environmental fate of phthalate esters a literature review. Chemosphere 35:667–749CrossRefGoogle Scholar
  31. Shi W, Guo J, Zhou Y, Deng D, Han Z, Zhang X, Yu H, Giesy JP (2016) Phthalate esters on hands of office workers: estimating the influence of touching surfaces. Environ Sci Tech Let 4:1–5Google Scholar
  32. Siddique T, Okeke BC, Arshad M, Frankenberger WT (2003) Biodegradation kinetics of endosulfan by Fusarium ventricosum and a Pandoraea species. J Agr Food Chem 51:8015–8019CrossRefGoogle Scholar
  33. Tang WJ, Zhang LS, Fang Y, Zhou Y, Ye BC (2016) Biodegradation of phthalate esters by newly isolated Rhizobium sp. LMB-1 and its biochemical pathway of di-n-butyl phthalate. J Appl Microbiol 121:177–186CrossRefGoogle Scholar
  34. Wang Y, Fan Y, Gu JD (2003) Aerobic degradation of phthalic acid by Comamonas acidovoran Fy-1 and dimethyl phthalate ester by two reconstituted consortia from sewage sludge at high concentrations. World J Microbiol Biotechnol 19:811–815CrossRefGoogle Scholar
  35. Wang J, Zhang MY, Chen T, Zhu Y, Teng Y, Luo YM, Peter C (2015a) Isolation and Identiflcation of a di-(2-Ethylhexyl) phthalate-degrading bacterium and its role in the bioremediation of a contaminated soil. Pedosphere 25:202–211CrossRefGoogle Scholar
  36. Wang X, Wang Q, Li S, Li W (2015b) Degradation pathway and kinetic analysis for p-xylene removal by a novel Pandoraea sp. strain WL1 and its application in a biotrickling filter. J Hazard Mater 288:17–24CrossRefGoogle Scholar
  37. Wang J, Lv SH, Zhang MY, Chen GC, Zhu TB, Zhang S, Teng Y, Christie P, Luo YM (2016) Effects of plastic film residues on occurrence of phthalates and microbial activity in soils. Chemosphere 151:171–177CrossRefGoogle Scholar
  38. Wang Y, Li F, Ruan X, Song J, Lv L, Chai LY, Yang ZH, Luo L (2017) Biodegradation of di-n-butyl phthalate by bacterial consortium LV-1 enriched from river sludge. PLoS One 12:e0178213CrossRefGoogle Scholar
  39. Wu X, Liang R, Dai Q, Jin D, Wang Y, Chao W (2010) Complete degradation of di-n-octyl phthalate by biochemical cooperation between Gordonia sp. strain JDC-2 and Arthrobacter sp. strain JDC-32 isolated from activated sludge. J Hazard Mater 176:262–268CrossRefGoogle Scholar
  40. Wu X, Wang Y, Dai Q, Liang R, Jin D (2011a) Isolation and characterization of four di-n-butyl phthalate (DBP)-degrading Gordonia sp. strains and cloning the 3,4-phthalate dioxygenase gene. World Jo Microbiol Biotechnol 27:2611–2617CrossRefGoogle Scholar
  41. Wu X, Wang Y, Liang R, Dai Q, Jin D, Chao W (2011b) Biodegradation of an endocrine-disrupting chemical di-n-butyl phthalate by newly isolated Agrobacterium sp. and the biochemical pathway. Process Biochem 46:1090–1094CrossRefGoogle Scholar
  42. Yu ZG, Zhang C, Zheng ZH, Hu L, Li XM, Yang ZZ, Ma C, Zeng GM (2017) Enhancing phosphate adsorption capacity of SDS-based magnetite by surface modification of citric acid. Appl Surf Sci 403:413–425CrossRefGoogle Scholar
  43. Yuan SY, Huang IC, Chang BV (2010) Biodegradation of dibutyl phthalate and di-(2-ethylhexyl) phthalate and microbial community changes in mangrove sediment. J Hazard Mater 184:826–831CrossRefGoogle Scholar
  44. Zhang HG, Sun GS, Sun L, Zhou ZF, Zhang SK, Sui H (2013) Preliminary study on phthalic acid esters pollution of typical plastic mulched crops soils. Environ Monit China 29:60–63 (in Chinese)Google Scholar
  45. Zhang C, Yu ZG, Zeng GM, Jiang M, Yang ZZ, Cui F, Zhu MY, Shen LQ, Hu L (2014) Effects of sediment geochemical properties on heavy metal bioavailability. Environ Int 73:270–281CrossRefGoogle Scholar
  46. Zhang Y, Wang P, Wang L, Sun G, Zhao J, Zhang H, Du N (2015) The influence of facility agriculture production on phthalate esters distribution in black soils of northeast China. Sci Total Environ 506:118–125CrossRefGoogle Scholar
  47. Zhao HM, Du H, Feng NX, Xiang L, Li YW, Li H, Cai QY, Mo CH (2016a) Biodegradation of di-n-butylphthalate and phthalic acid by a novel Providencia sp. 2D and its stimulation in a compost-amended soil. Biol fert soils 52:65–76CrossRefGoogle Scholar
  48. Zhao HM, Du H, Lin J, Chen XB, Li YW, Li H, Cai QY, Mo CH, Qin HM, Wong MH (2016b) Complete degradation of the endocrine disruptor di-(2-ethylhexyl) phthalate by a novel Agromyces sp. MT-O strain and its application to bioremediation of contaminated soil. Sci Total Environ 562:170–178CrossRefGoogle Scholar

Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  • Jing Yang
    • 1
  • Chuling Guo
    • 1
    • 2
    • 3
  • Shasha Liu
    • 1
  • Weiting Liu
    • 1
  • Han Wang
    • 1
  • Zhi Dang
    • 1
    • 2
  • Guining Lu
    • 1
    • 2
  1. 1.School of Environment and EnergySouth China University of TechnologyGuangzhouPeople’s Republic of China
  2. 2.The Key Lab of Pollution Control and Ecosystem Restoration in Industry ClustersMinistry of EducationGuangzhouPeople’s Republic of China
  3. 3.Guangdong Provincial Engineering and Technology Research Center for Environmental Risk Prevention and Emergency DisposalSouth China University of TechnologyGuangzhouChina

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