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Environmental Science and Pollution Research

, Volume 25, Issue 17, pp 16767–16775 | Cite as

Identification and quantitation of semi-crystalline microplastics using image analysis and differential scanning calorimetry

  • Mauricio Rodríguez Chialanza
  • Ignacio Sierra
  • Andrés Pérez Parada
  • Laura Fornaro
Research Article
  • 265 Downloads

Abstract

There are several techniques used to analyze microplastics. These are often based on a combination of visual and spectroscopic techniques. Here we introduce an alternative workflow for identification and mass quantitation through a combination of optical microscopy with image analysis (IA) and differential scanning calorimetry (DSC). We studied four synthetic polymers with environmental concern: low and high density polyethylene (LDPE and HDPE, respectively), polypropylene (PP), and polyethylene terephthalate (PET). Selected experiments were conducted to investigate (i) particle characterization and counting procedures based on image analysis with open-source software, (ii) chemical identification of microplastics based on DSC signal processing, (iii) dependence of particle size on DSC signal, and (iv) quantitation of microplastics mass based on DSC signal. We describe the potential and limitations of these techniques to increase reliability for microplastic analysis. Particle size demonstrated to have particular incidence in the qualitative and quantitative performance of DSC signals. Both, identification (based on characteristic onset temperature) and mass quantitation (based on heat flow) showed to be affected by particle size. As a result, a proper sample treatment which includes sieving of suspended particles is particularly required for this analytical approach.

Keywords

Microplastics Image analysis Thermal analysis DSC 

Notes

Acknowledgments

Heinkel Bentos Pereira is acknowledged for the construction of PVC sieving system.

Funding information

Authors acknowledge founding from Programa de Desarrollo de Ciencias Básicas (PEDECIBA) and Cristalpet and Nicoll (Montevideo, Uruguay) for supplying plastics samples.

Supplementary material

11356_2018_1846_MOESM1_ESM.docx (733 kb)
ESM 1 (DOCX 733 kb)

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Centro Universitario Regional del Este (CURE), Universidad de la República (UdelaR)RochaUruguay
  2. 2.Departamento de Desarrollo Tecnológico – DDT, Centro Universitario Regional del Este (CURE)Universidad de la República (UdelaR)RochaUruguay

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