Environmental Science and Pollution Research

, Volume 25, Issue 18, pp 17383–17392 | Cite as

Valorisation of tuna processing waste biomass: isolation, purification and characterisation of four novel antioxidant peptides from tuna by-product hydrolysate

  • Sami Saidi
  • Mongi Saoudi
  • Raja Ben Amar
Research Article


Tuna protein hydrolysate (TPH) was prepared by hydrolysis with Prolyve BS and fractionated by membranes process. The antioxidant activities of recovered peptide fractions were evaluated. Four novel antioxidant peptides that were isolated from nanofiltration retentate exhibited the highest antioxidant activity, using gel chromatography and reversed phase high-performance liquid chromatography. The amino acid sequences of isolated peptides were identified as Tyr-Glu-Asn-Gly-Gly (P2), Glu-Gly-Tyr-Pro-Trp-Asn (P4), Tyr-Ile-Val-Tyr-Pro-Gly (P7) and Trp-Gly-Asp-Ala-Gly-Gly-Tyr-Tyr (P8) with molecular weights of 538.46, 764.75, 710.78 and 887.85 Da, respectively. P2, P4, P7 and P8 exhibited good scavenging activities on hydroxyl radical (IC50 0.41, 0.327, 0.17 and 0.042 mg/ml), DPPH radical (IC50 0.666, 0.326, 0.451 and 0.377 mg/ml) and superoxide radical (IC50 0.536, 0.307, 0.357 and 0.115 mg/ml). P7 was effective against lipid peroxidation in the model system. The isolated peptides might be useful used as natural food additive in food industry and formulation of nutritional products.


Valorisation Tuna waste biomass hydrolysate Antioxidant activity Membrane fractionation Peptide Amino acid composition Amino acid sequence 


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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Laboratory of Materials Science and Environment, Faculty of Science of SfaxUniversity of SfaxSfaxTunisia
  2. 2.Animal Ecophysiology Laboratory, Faculty of Sciences of SfaxUniversity of SfaxSfaxTunisia

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