Assessment of biotoxicity of Cu nanoparticles with respect to probiotic strains of microorganisms and representatives of the normal flora of the intestine of broiler chickens

Abstract

Copper nanoparticle Cu (d = 55 ± 15 nm) and CuO nanoparticles (d = 90 ± 10 nm) were used in the studies (OOO Platina, Russia). Using the method of pure cultures, we extracted Lactobacillus, Enterococcus, and Enterobacterium from the intestines of broilers. Additionally, strains of Bacillus subtilis 10641 and Bifidobacterium were involved in probiotic strains. The data obtained in the course of the study testify to the insignificant biotoxicity of copper nanoparticles with respect to representatives of the genera Lactobacillus (30 to 15 μg/ml) and Bifidobacterium (30 μg/ml), with the most sensitive bacteria being the genus Lactobacillus, for which a concentration of 7.5 μg/ml was subinhibitory. The second stage was the study using method of agar wells. In the course of the experiment, we obtained results confirming the data of the research by the serial dilution method. In this case, as in the first case, the data indicate the insignificant biotoxicity of copper nanoparticles in relation to representatives of the genera Lactobacillus and Bifidobacterium. We have studied the bioaccumulating ability of microorganisms of the studied metals. In all the studies carried out, as in the first series of experiments, representatives of the genera Lactobacillus and Bifidobacterium with the lowest bioaccumulative ability were the most sensitive to copper nanoparticles and were 3.1 and 8.2%, respectively. The use of nanoparticles as a component of the fodder additive in small concentrations does not adversely affect not only the probiotic strains, but also the main representatives of the normoflora (Lactobacillus, Enterococcus, and Enterobacterium) of the poultry, the positive effect of the copper nanoparticles being directly related to low level of dissociation of nanoparticles, since biologically active ions will be released much more slowly, thereby creating a prolonged effect of exposure.

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Funding

This research was carried out with the financial support of the Russian Ministry of Science and Education as part of the base part of the state task to carry out research projects in the Orenburg State University (No. 6.6811.2017/Ch).

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Correspondence to Irina Aleksandrovna Gavrish.

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The studies were carried out under the conditions of the experimental and biological clinic of the Orenburg State University on broiler chickens (Smena 8). Experimental studies were conducted in accordance with the instructions recommended by the Russian Regulations, 1987, and “The Guide for the Care and Use of Laboratory Animals (National Academy Press Washington, D.C. 1996).”

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Responsible editor: Philippe Garrigues

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Sizentsov, A.N., Kvan, O.V., Miroshnikova, E.P. et al. Assessment of biotoxicity of Cu nanoparticles with respect to probiotic strains of microorganisms and representatives of the normal flora of the intestine of broiler chickens. Environ Sci Pollut Res 25, 15765–15773 (2018). https://doi.org/10.1007/s11356-018-1761-4

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Keywords

  • Cu nanoparticles
  • Probiotics
  • Bioaccumulation
  • Lactobacillus
  • Enterococcus
  • Enterobacterium