Biochemical and morphological biomarkers of the liver damage in the Neotropical fish, Piaractus mesopotamicus, injected with crude extract of cyanobacterium Radiocystis fernandoi

  • Driele Tavares
  • Marcelo Gustavo Paulino
  • Ana Paula Terezan
  • João Batista Fernandes
  • Alessandra Giani
  • Marisa Narciso Fernandes
Research Article
  • 59 Downloads

Abstract

Cyanobacterial proliferation in river and lakes is the result of eutrophication. The cyanobacterium Radiocystis fernandoi strain R28 produces mostly two MC variants MC-RR and MC-YR and small amounts of other oligopeptides, but does not produce MC-LR. The present study investigated the hepatotoxic potential of the crude extract of the R. fernandoi strain R28 on the Neotropical fish, Piaractus mesopotamicus, at 3, 6, and 24 h after intraperitoneal injection (100 μg MC-LR equivalent per kg−1 body mass) using biochemical and morphological biomarkers of liver damage. Although the protein phosphatases PP1 and PP2A were not inhibited during the 24-h treatment, liver parenchyma and hepatocyte structure were disrupted. Alkaline phosphatase increased at 3 h post-injection and decreased after 24 h; alanine aminotransferase and aspartate aminotransferase increased in a time-dependent manner up to 24 h indicating impaired liver function. Progressive histopathological changes were consistent with biochemical results demonstrating alterations in liver structure and function. In conclusion, the crude extract of R. fernandoi strain R28 has high hepatotoxic potential and can severely compromise fish health.

Keywords

Bilirubin Liver histopathology Microcystins Phosphatases Teleost Transaminases 

Notes

Acknowledgments

This study was supported by the Energetic Company of Minas Gerais (CEMIG, Grant no. GT 346) and São Paulo Research Foundation (FAPESP, Grant no. 2012/00728-1). D. Tavares and M.G. Paulino acknowledge the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) and FAPESP (Grant no. 2012/00728-1), respectively, for scholarships.

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  • Driele Tavares
    • 1
  • Marcelo Gustavo Paulino
    • 1
    • 2
  • Ana Paula Terezan
    • 3
  • João Batista Fernandes
    • 3
  • Alessandra Giani
    • 4
  • Marisa Narciso Fernandes
    • 1
    • 5
  1. 1.Department of Physiological SciencesFederal University of São CarlosSão CarlosBrazil
  2. 2.Federal University of Tocantins, Campus AraguainaAraguaínaBrazil
  3. 3.Department of ChemistryFederal University of São CarlosSão CarlosBrazil
  4. 4.Department of BotanyFederal University of Minas GeraisBelo HorizonteBrazil
  5. 5.Department of Physiological SciencesFederal University of São CarlosSão CarlosBrazil

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