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Detection of 2-aminoethylphosphonic acid in suspended particles in an ultraoligotrophic lake: a two-dimensional nuclear magnetic resonance (2D-NMR) study

  • Ryuichiro Shinohara
  • Tomoya Iwata
  • Yoshiki Ikarashi
  • Tomoharu Sano
Letter to the Editor

Abstract

Particulate organic phosphorus (P) compounds were examined in ultraoligotrophic Lake Saiko, Japan. A cartridge filter was used to collect sufficient amount of suspended particles for analysis by a two-dimensional NMR (1H-31P heteronuclear multiple bond correlation). 2-Aminoethylphosphonic acid (2-AEP), a phosphonate, was detected in suspended particles in Lake Saiko. The identity of the phosphonate was confirmed by comparison with a commercially available compound. Because 2-AEP is bioavailable, microorganisms can store and use this compound under extremely P-limited conditions. This is the first study to detect 2-AEP in an ultra-oligotrophic environment.

Keywords

Two-dimensional NMR 2-aminoethylphosphonic acid Ultra-oligotrophic 

Notes

Acknowledgments

We thank Mariko Watanabe for her help with the NMR analyses.

Funding information

The current study was financially supported by a Grant-in-aid for Young Scientists (A) (15H05533) and a Grant-in-aid (B) (16H02935) from the Japan Society for the Promotion of Sciences (JSPS).

Supplementary material

11356_2018_1744_MOESM1_ESM.docx (354 kb)
ESM 1 (DOCX 354 kb)

References

  1. Ahlgren J, Tranvik L, Gogoll A, Waldebäck M, Markides K, Rydin E (2005) Sediment depth attenuation of biogenic phosphorus compounds measured by 31P NMR. Environ Sci Technol 39:867–872CrossRefGoogle Scholar
  2. Benitez-Nelson C (2015) The missing link in oceanic phosphorus cycling? Science 348:759–760CrossRefGoogle Scholar
  3. Beversdorf L, White A, Björkman K, Letelier R, Karl D (2010) Phosphonate metabolism of Trichodesmium IMS101 and the production of greenhouse gases. Limnol Oceanogr 55:1768–1778CrossRefGoogle Scholar
  4. Cade-Menun BJ, Paytan A (2010) Nutrient temperature and light stress alter phosphorus and carbon forms in culture-grown algae. Mar Chem 121:27–36CrossRefGoogle Scholar
  5. Cade-Menun B, Preston C (1996) A comparison of soil extraction procedures for 31P NMR spectroscopy. Soil Sci 161:770–785CrossRefGoogle Scholar
  6. Clark LL, Ingall ED, Benner R (1998) Marine phosphorus is selectively remineralized. Nature 393:426CrossRefGoogle Scholar
  7. Diaz J, Ingall E, Benitez-Nelson C, Paterson D, de Jonge MD, McNulty I, Brandes JA (2008) Marine polyphosphate: a key player in geologic phosphorus sequestration. Science 320:652–655CrossRefGoogle Scholar
  8. Doolette AL, Smernik RJ, Dougherty WJ (2009) Spiking improved solution phosphorus-31 nuclear magnetic resonance identification of soil phosphorus compounds. Soil Sci Soc Am J 73:919–927CrossRefGoogle Scholar
  9. Dyhrman S, Chappell P, Haley S, Moffett J, Orchard E, Waterbury J, Webb E (2006) Phosphonate utilization by the globally important marine diazotroph Trichodesmium. Nature 439:68–71CrossRefGoogle Scholar
  10. He Z, Olk DC, Cade-Menun BJ (2011) Forms and lability of phosphorus in humic acid fractions of hord silt loam soil. Soil Sci Soci Am J 75:1712–1722CrossRefGoogle Scholar
  11. Kamat SS, Williams HJ, Dangott LJ, Chakrabarti M, Raushel FM (2013) The catalytic mechanism for aerobic formation of methane by bacteria. Nature 497:132–136CrossRefGoogle Scholar
  12. Karl DM, Beversdorf L, Björkman KM, Church MJ, Martinez A, Delong EF (2008) Aerobic production of methane in the sea. Nat Geosci 1:473–478CrossRefGoogle Scholar
  13. Kolowith LC, Ingall ED, Benner R (2001) Composition and cycling of marine organic phosphorus. Limnol Oceanogr 46:309–320CrossRefGoogle Scholar
  14. Kononova S, Nesmeyanova M (2002) Phosphonates and their degradation by microorganisms. Biochem Mosc 67:184–195CrossRefGoogle Scholar
  15. Lean D (1973) Phosphorus dynamics in lake water. Science 179:678–680CrossRefGoogle Scholar
  16. Metcalf WW, Griffin BM, Cicchillo RM, Gao J, Janga SC, Cooke HA, Circello BT, Evans BS, Martens-Habbena W, Stahl DA (2012) Synthesis of methylphosphonic acid by marine microbes: a source for methane in the aerobic ocean. Science 337:1104–1107CrossRefGoogle Scholar
  17. Pasek MA, Sampson JM, Atlas Z (2014) Redox chemistry in the phosphorus biogeochemical cycle. Proc Nat Acad Sci 111:15468–15473CrossRefGoogle Scholar
  18. Paytan A, Cade-Menun BJ, McLaughlin K, Faul KL (2003) Selective phosphorus regeneration of sinking marine particles: evidence from 31 P-NMR. Mar Chem 82:55–70CrossRefGoogle Scholar
  19. Reitzel K, Ahlgren J, DeBrabandere H, Waldeb ck M, Gogoll A, Tranvik L, Rydin E (2007) Degradation rates of organic phosphorus in lake sediment. Biogeochemistry 82:15–28CrossRefGoogle Scholar
  20. Repeta DJ, Ferrón S, Sosa OA, Johnson CG, Repeta LD, Acker M, DeLong EF, Karl DM (2016) Marine methane paradox explained by bacterial degradation of dissolved organic matter. Nat Geosci 9:884–887CrossRefGoogle Scholar
  21. Roseberg H, La Nauze JM (1967) The metabolism of phosphanates by microorganisms. The transport of aminoethylphosphonic acid in Basillus cereus. Biochim Biophys Acta Gen Subj 141:79–90CrossRefGoogle Scholar
  22. Shinohara R, Imai A, Kawasaki N, Komatsu K, Kohzu A, Miura S, Sano T, Satou T, Tomioka N (2012) Biogenic phosphorus compounds in sediment and suspended particles in a shallow eutrophic lake: a 31P-nuclear magnetic resonance (31P NMR) study. Environ Sci Technol 46:10572–10578CrossRefGoogle Scholar
  23. Shinohara R, Imai A, Kohzu A, Tomioka N, Furusato E, Satou T, Sano T, Komatsu K, Miura S, Shimotori K (2016) Dynamics of particulate phosphorus in a shallow eutrophic lake. Scie Total Environ 563:413–423CrossRefGoogle Scholar
  24. Turner BL, Mahieu N, Condron LM (2003) Phosphorus-31 nuclear magnetic resonance spectral assignments of phosphorus compounds in soil NaOH-EDTA extracts. Soil Sci Soc Am J 67:497–510CrossRefGoogle Scholar
  25. Yoshimura T, Nishioka J, Saito H, Takeda S, Tsuda A, Wells ML (2007) Distributions of particulate and dissolved organic and inorganic phosphorus in North Pacific surface waters. Mar Chem 103:112–121CrossRefGoogle Scholar

Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  1. 1.National Institute for Environmental StudiesTsukubaJapan
  2. 2.Department of Environmental SciencesUniversity of YamanashiKofuJapan

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