Environmental Science and Pollution Research

, Volume 25, Issue 30, pp 30739–30743 | Cite as

Detection of 2-aminoethylphosphonic acid in suspended particles in an ultraoligotrophic lake: a two-dimensional nuclear magnetic resonance (2D-NMR) study

  • Ryuichiro ShinoharaEmail author
  • Tomoya Iwata
  • Yoshiki Ikarashi
  • Tomoharu Sano
Letter to the Editor


Particulate organic phosphorus (P) compounds were examined in ultraoligotrophic Lake Saiko, Japan. A cartridge filter was used to collect sufficient amount of suspended particles for analysis by a two-dimensional NMR (1H-31P heteronuclear multiple bond correlation). 2-Aminoethylphosphonic acid (2-AEP), a phosphonate, was detected in suspended particles in Lake Saiko. The identity of the phosphonate was confirmed by comparison with a commercially available compound. Because 2-AEP is bioavailable, microorganisms can store and use this compound under extremely P-limited conditions. This is the first study to detect 2-AEP in an ultra-oligotrophic environment.


Two-dimensional NMR 2-aminoethylphosphonic acid Ultra-oligotrophic 



We thank Mariko Watanabe for her help with the NMR analyses.

Funding information

The current study was financially supported by a Grant-in-aid for Young Scientists (A) (15H05533) and a Grant-in-aid (B) (16H02935) from the Japan Society for the Promotion of Sciences (JSPS).

Supplementary material

11356_2018_1744_MOESM1_ESM.docx (354 kb)
ESM 1 (DOCX 354 kb)


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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  1. 1.National Institute for Environmental StudiesTsukubaJapan
  2. 2.Department of Environmental SciencesUniversity of YamanashiKofuJapan

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