Transcriptome analysis reveals the molecular mechanism of hepatic metabolism disorder caused by chromium poisoning in chickens
- 176 Downloads
Chromium (Cr) is one of the most important environmental pollutants which are released into the environment due to their wide usage in numerous industries. The excess of Cr (VI) can induce hepatotoxicity, while the molecular mechanism that is involved in Cr (VI)-induced hepatotoxicity is unclear. We demonstrated the induction of chromium poisoning model in chickens to identify the differentially expressed genes (DEGs), and their functions were analyzed under different physiological and pathological conditions. Histopathological examination and transcriptome data for chromium-poisoned livers and control livers were annotated with Illumina® HiSeq 2000. The histopathological examination in chromium poisoning groups showed diapedesis, hemolysis, degeneration, nucleus pycnosis, and central phlebectasia in the liver. A total of 334 genes were upregulated and 509 genes were downregulated. The most strongly upregulated genes were HKDC1, DDX4, ACACA, FDFT1, CYYR1, PPP1R3C, and SLC16A14, while the most downregulated genes were MYBPC3, CCKAR, PCK1, and CPT1A. A Gene Ontology (GO) term with the highest enrichment of DEGs is small molecule metabolic process. In cell component domain, the term with the highest enrichment is extracellular matrix. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways showed that glucose metabolism, lipid metabolism, and protein metabolism were the most important metabolic pathways in the liver. The current study first time provides important clues and evidence for identifying the differentially expressed genes in livers due to Cr (VI)-induced liver injury in chickens.
KeywordsTranscriptome Chromium Hepatotoxicity Hepatic metabolism Liver
X.T., H.Z., Y.Z., X.W., Z.C., M.L., and D.Z. were responsible for study conception and design; H.Z., D.Z., K.M., X.L., M.I., and M.T.J. were involved in the drafting of the manuscript.
This study was supported by the National Key R&D Program of China (2016YFD0501208), National Natural Science Foundation of China (No: 30700588) and Hubei Provincial Natural Science Foundation of China (Grant No: 2014CFB244).
Compliance with ethical standards
All the experiments were performed after the approval of the Institutional Animal Welfare and Research Ethics Committee of Huazhong Agricultural University Wuhan, China (approval number 31272556). All animal experiments and methods were conducted under the relevant procedure of Proclamation of the Standing Committee of Hubei People’s Congress (No. 29), China.
Conflicts of interest
The authors declare that they have no conflict of interest.
- Bislimi K, Behluli A, Bojniku H, Halili J, Mazreku I, Halili F (2012) Accumulation of chromium in hybro chickens liver treated with Cr(vi) and vitamin C. Int J Ecosyst Ecol Sci 2:201–206Google Scholar
- Castro MP, de Moraes FR, Fujimoto RY, Da CC, Belo MA, de Moraes JR (2014) Acute toxicity by water containing hexavalent or trivalent chromium in native Brazilian fish, Piaractus mesopotamicus: anatomopathological alterations and mortality. Bull Environ Contam Toxicol 92(2):213–219CrossRefGoogle Scholar
- Huang S, Peng W, Jiang X, Shao K, Xia L, Tang Y, Qiu J (2014) The effect of chromium picolinate supplementation on the pancreas and macroangiopathy in type ii diabetes mellitus rats. J Diabetes Res 9:717219Google Scholar
- Khan AZ, Kumbhar S, Hamid M, Afzal S, Parveen F, Liu Y, Shu H, Mengistu BM, Huang K (2016) Effects of selenium-enriched robiotics on heart lesions by influencing the mRNA expressions of selenoproteins and heat shock proteins in heat stressed broiler chickens. Pak Vet J 36(4):460–464Google Scholar
- Matsumoto ST, Marin-Morales MA (2005) Toxic and genotoxic effects of trivalent and hexavalent chromium—a review. Rev Bras Toxicol 18(1):77–85Google Scholar
- Mehmood K, Zhang H, Iqbal MK, Rehman MU, Shahzad M, Li K, Huang S, Nabi F, Zhang L, Li J (2017) In vitro effect of apigenin and danshen in tibial dyschondroplasia through inhibition of heat-shock protein 90 and vascular endothelial growth factor expressions in avian growth plate cells. Avian Dis 61(3):372–377CrossRefGoogle Scholar
- Mehmood K, Zhang H, Li K, Wang L, Rehman MU, Nabi F, Iqbal MK, Luo H, Shahzad M, Li J (2018) Effect of tetramethylpyrazine on tibial dyschondroplasia incidence, tibial angiogenesis, performance and characteristics via HIF-1α/VEGF signaling pathway in chickens. Sci Rep 8(1):2495CrossRefGoogle Scholar
- NRC (National Research Council) (2001) Nutrient requirements of poultry, 9th Rev. Ed. National Academy Press, Washington DCGoogle Scholar
- Sarkar S, Satheshkumar A, Pradeepa N, Premkumar R, Dist C, Tamil N (2013) Hexavalent chromium (cr (vi)) removal by live mycelium of a Trichoderma harzianum strain. Mol Soil Biol 4(1):1–6Google Scholar
- Sr JPW, Qin Q (2013) Hexavalent chromium and DNA, biological implications of interaction. Springer New York, New YorkGoogle Scholar
- Wang Q, Liu M, Xu L, Wu Y, Huang Y (2017b) Transcriptome analysis reveals the molecular mechanism of hepatic fat metabolism disorder caused by Muscovy duck reovirus infection. Avian Pathol 10:1–13Google Scholar
- Wu Q, Chen Z, Sun W, Deng T, Chen M (2016) De novo sequencing of the leaf transcriptome reveals complex light-responsive regulatory networks in Camellia sinensis cv. Baijiguan. Front Plant Sci 7:332Google Scholar
- Zhuang Y, Liu P, Zhang CY, Ye S, Hu G, Cao HB (2016) Effect of cadmium on the concentration of ceruloplasmin and its mRNA expression in goats under molybdenum stress. Pak Vet J 36(2):209–213Google Scholar