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Recovery and characterization of proteins from pangas (Pangasius pangasius) processing waste obtained through pH shift processing

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Abstract

Study was conducted to recover proteins from pangas (Pangasius pangasius) processing waste (fillet frames) using pH shift method and to characterize the recovered isolates. pH 2.0 from acidic range and pH 13.0 from alkaline range were found to have maximum protein recovery (p < 0.05). During the recovery process, acidic pH (pH 2.0) was found to have minimal effect on proteins resulting in more stable isolates and strong protein gels. Alkaline pH (pH 13.0) caused protein denaturation resulting in less stable proteins and poor gel network. Both acidic and alkaline-aided processing caused significant (p < 0.05) reductions in total lipid, myoglobin, and pigment content thus by resulting in whiter protein isolates and gels. The content of total essential amino acids increased during pH shift processing, indicating the enrichment of essential amino acids. No microbial counts were detected in any of the isolates prepared using acid and alkaline extraction methods. pH shift processing was found to be promising in the utilization of fish processing waste for the recovery of functional proteins from pangas processing waste thus by reducing the supply demand gap as well pollution problems.

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References

  • AOAC (2000) Association of official analytical chemists, 16th edn. Washington, DC

    Google Scholar 

  • Azadian M, Moosavi-Nasab M, Abedi E (2012) Comparison of functional properties and SDS-PAGE patterns between fish protein isolate and surimi produced from silver carp. Eur Food Res Technol 235(1):83–90. https://doi.org/10.1007/s00217-012-1721-z

    Article  CAS  Google Scholar 

  • Batista I (1999) Recovery of proteins from fish waste products by alkaline extraction. Eur Food Res Technol 210(2):84–89. https://doi.org/10.1007/s002170050539

    Article  CAS  Google Scholar 

  • Batista I, Pires C, Nelhas R (2007) Extraction of sardine proteins by acidic and alkaline solubilisation. Food Sci Technol Int 13(3):189–194. https://doi.org/10.1177/1082013207079619

    Article  CAS  Google Scholar 

  • Bidlingmeyer BA, Cohen SA, Tarvin TL (1984) Rapid analysis of amino acids using precolumn derivatisation. J Chromatogr 336(1):93–104. https://doi.org/10.1016/S0378-4347(00)85133-6

    Article  CAS  Google Scholar 

  • Chaijan M, Undeland I (2015) Development of a new method for determination of total haem protein in fish muscle. Food Chem 173:1133–1141. https://doi.org/10.1016/j.foodchem.2014.11.010

    Article  CAS  Google Scholar 

  • Chaijan M, Benjakul S, Visessanguan W, Faustman C (2006) Physicochemical properties, gel forming ability and myoglobin content of sardine (Sardinnella gibbosa) and mackerel (Rastrelliger kanagurta) surimi produced by conventional method and alkaline solubilization process. Eur Food Res Technol 222(1-2):58–63. https://doi.org/10.1007/s00217-005-0091-1

    Article  CAS  Google Scholar 

  • Chen YC, Tou JC, Jaczynski J (2007) Protein recovery from rainbow trout (Oncorhynchus mykiss) processing byproducts via isoelectric solubilization/precipitation and its gelation properties as affected by functional additives. J Agric Food Chem 55(22):9079–9088. https://doi.org/10.1021/jf071992w

    Article  CAS  Google Scholar 

  • Cortes-Ruis J, Pachero-Aguilar R, Garcia-Sanchez G, Lugo-Sanches ME (2001) Functional characterization of a protein concentrate from bristly sardine made under acidic conditions. J Aquat Food Prod Technol 10(4):5–23. https://doi.org/10.1300/J030v10n04_02

    Article  Google Scholar 

  • Fatin NS, Huda N, David W (2015) Physicochemical properties of Japanese scad (Decapterus Maruadsi) surimi prepared using the acid and alkaline solubilization methods. Int J Sci Eng Res 6(4):141–147

    Google Scholar 

  • Feng YM, Hultin HO (2001) Effect of pH on the rheological and structural properties of gels of water-washed chicken-breast muscle at physiological ionic strength. J Agric Food Chem 49(8):3927–3935. https://doi.org/10.1021/jf001021f

    Article  CAS  Google Scholar 

  • Freitas IR, Gauterio GV, Rios DG, Prentice C (2011) Functionality of protein isolates from argentine anchovy (Engraulis anchoita) residue using pH shift processing. J Food Sci Eng 1:374–378

    CAS  Google Scholar 

  • Freitas IR, Cortez-Wega WR, Prentice C (2015) Evaluation of properties of protein recovered from fish muscle by acid solubilization process. Int Food Res J 22(3):1067–1073

    CAS  Google Scholar 

  • Gehring CK, Gigliotti JC, Moritz JS, Tou JC, Jaczynski J (2010) Functional and nutritional characteristics of proteins and lipids recovered by isoelectric processing of fish by-products and low-value fish: a review. Food Chem 124:422–431

    Article  Google Scholar 

  • Haard NF (1992) Control of chemical composition and food quality attributes of cultured fish. Food Res Int 25(4):289–307. https://doi.org/10.1016/0963-9969(92)90126-P

    Article  CAS  Google Scholar 

  • Hamm R (1994) The influence of pH on the protein net charge in the myofibrillar system. Reciprocal Meat Conf Proc 47:5–9

    Google Scholar 

  • Harlow E, Lane D (1988) Antibodies: a laboratory manual. Cold Spring Harbor Laboratory Press, New York

    Google Scholar 

  • Huang L, Chen Y, Morrissey MT (1997) Coagulation of fish proteins from frozen fish mince wash water by ohmic heating. J Food Process Eng 20(4):285–300. https://doi.org/10.1111/j.1745-4530.1997.tb00423.x

    Article  Google Scholar 

  • Hultin HO, Kelleher SD (1999) Process for isolating a protein composition from a muscle source and protein composition. US Patent 6,005,073

  • Hultin HO, Kelleher SD, Feng YM, Kristinsson HG, Richards MP, Undenand IA (2000) High efficiency alkaline protein extraction. US Patent 6,136,959

  • Huss HH (1983) Fresk Fisk. K valitet Og Holdbarhed. [Fresh fish. Quality and quality changes]. Ministry of Fisheries, Technological laboratory, Lyngby

  • Jafarpour SA, Shabanpour B, Filabadi SS (2013) Biochemical properties of fish protein isolate (FPI) from silver carp (Hypophthalmychthis molitrix) by application of acid-alkali process compared to traditional prepared surimi. Ecopersia 1(3):315–327

    Google Scholar 

  • Jay JM (1986) Modern food microbiology. Van Nostrand Reinhold Company, New York

    Google Scholar 

  • Jongjareonrak A, Rawdkuen S, Chaijan M, Benjakul S, Osako K, Tanaka M (2010) Chemical compositions and characterization of skin gelatin from farmed giant catfish (Pangasianodon gigas). LWT Food Sci Technol 43(1):161–165. https://doi.org/10.1016/j.lwt.2009.06.012

    Article  CAS  Google Scholar 

  • Kelleher SD, Hultin HO (2000) Functional chicken muscle protein isolates prepared using low ionic strength, acid solubilisation/precipitation. Reciprocal Meat Conf Proc 3:76–81

    Google Scholar 

  • Kinsella JE (1976) Functional properties of proteins in foods, a survey. CRC Crit Rev Food Sci Nutr 7(3):219–280. https://doi.org/10.1080/10408397609527208

    Article  CAS  Google Scholar 

  • Kristinsson H, Demir N (2003) Functional fish protein ingredients from fish species of warm and temperate waters: comparison of acid- and alkali-aided processing vs. conventional surimi processing. In: Betchel PJ (ed) Advances in seafood byproducts 2002 Conference Proceedings, Alaska Sea Grant College Program University of Alaska, pp. 277–295

  • Kristinsson H, Ingadottir B (2006) Recovery and properties of muscle proteins extracted from tilapia (Oreochromis niloticus) light muscle by pH shift processing. J Food Sci 1(3):E132–E141

    Article  Google Scholar 

  • Kristinsson HG, Liang Y (2006) Effect of pH-shift processing and surimi processing on Atlantic croaker (Micropogonias undulates) muscle proteins. J Food Sci 71(5):C304–C312. https://doi.org/10.1111/j.1750-3841.2006.00046.x

    Article  CAS  Google Scholar 

  • Kristinsson H, Theodore AE, Demir N, Ingadottir B (2005) A comparative study between acid- and alkali-aided processing and surimi processing for the recovery of proteins from channel catfish muscle. J Food Sci 70(4):C298–C306

    Article  CAS  Google Scholar 

  • Kudo G, Okada M, Miyauchi D (1973) Gel-forming capacity of washed and unwashed flesh of some Pacific coast species of fish. Mar Fish Rev 32:10–15

    Google Scholar 

  • Laemmli UK (1970) Cleavage of structural proteins during assembly of the head of bacteriophage T4. Nature 227(5259):680–685. https://doi.org/10.1038/227680a0

    Article  CAS  Google Scholar 

  • Lubana GK, Kaur B, Surasani VKR (2016) Quality changes in fresh rohu (Labeo rohita) cutlets added with fibers from ragi, oat and jowar. Nutr Food Sci 46(4):571–582. https://doi.org/10.1108/NFS-02-2016-0023

    Article  Google Scholar 

  • Marmon SK, Undeland I (2010) Protein isolation from gutted herring (Clupea harengus) using pH-shift processes. J Agric Food Chem 58(19):10480–10486. https://doi.org/10.1021/jf101057q

    Article  CAS  Google Scholar 

  • Mutilangi WAM, Panyam D, Kilara A (1996) Functional properties of properties of hydrolysates from proteolysis of heat-denatured whey protein isolate. J Food Sci 61(2):270–274. https://doi.org/10.1111/j.1365-2621.1996.tb14174.x

    Article  CAS  Google Scholar 

  • Niki H, Kato T, Deya E, Igarashi S (1985) Recovery of protein from effluent of fish meat in producing surimi and utilization of recovered protein. Nippon Suisan Gakkaishi 51(6):959–964. https://doi.org/10.2331/suisan.51.959

    Article  CAS  Google Scholar 

  • Nolsøe H, Undeland I (2009) The acid and alkaline solubilization process for the isolation of muscle proteins: state of art. Food Bioprocess Technol 2(1):1–27. https://doi.org/10.1007/s11947-008-0088-4

    Article  Google Scholar 

  • Nolsøe H, Marmon SK, Undeland I (2011) Application of filtration to recover solubilized proteins during ph-shift processing of blue whiting (Micromesistius poutassou); effects on protein yield and qualities of protein isolates. Open Food Sci J 5(1):1–9. https://doi.org/10.2174/1874256401105010001

    Article  Google Scholar 

  • Panpipat W, Chaijan M (2016) Biochemical and physicochemical characteristics of protein isolates from bigeye snapper (Priacanthus Tayenus) head by-product using pH shift method. Turkish. J Fish Aquat Sci 16:41–50

    Google Scholar 

  • Rawdkuen S, Sai-Ut S, Khamsorn S, Chaijan M, Benjakul S (2009) Biochemical and gelling properties of tilapia surimi and protein recovered using an acid-alkaline process. Food Chem 112(1):112–119. https://doi.org/10.1016/j.foodchem.2008.05.047

    Article  CAS  Google Scholar 

  • Reddy SVK (2016) Effect of formulation and processing methods on the quality and acceptability of cutlets made from minced meat of pangas (Pangasius pangasius). SAARC J Agric 14(1):25–36. https://doi.org/10.3329/sja.v14i1.29573

    Article  Google Scholar 

  • Robinson HW, Hogden CG (1940) The biuret reaction in the determination of serum proteins. J Biol Chem 135:707–725

    CAS  Google Scholar 

  • Sathe SK, Deshpande SS, Salunkhe DK (1982) Functional properties of lupin seed (Supinus mutabilis) proteins and protein concentrates. J Food Sci 7:191–197

    Google Scholar 

  • Shabanpour B, Etemadian Y, Taghipour B (2015) Physicochemical and rheological parameters changes for determining the quality of surimi and kamaboko produced by conventional, acid and alkaline solubilization process methods from common kilka (Clupeonella cultriventris caspia). Iran J Fish Sci 14(4):826–845

    Google Scholar 

  • Surasani VKR (2017) Influence of rohu (Labeo rohita) deboning by-product on composition, physical properties and sensorial acceptability of rohu cutlets. Nutr Food Sci 47(3):398–408. https://doi.org/10.1108/NFS-08-2016-0128

    Article  Google Scholar 

  • Surasani VKR, Tyagi A, Kudre T (2017) Recovery of proteins from rohu processing waste using ph shift method: characterization of isolates. J Aquat Food Prod Technol 26(3):356–365. https://doi.org/10.1080/10498850.2016.1186130

    Article  CAS  Google Scholar 

  • Szczesniak AS (2002) Texture is a sensory property. Food Qual Prefer 13(4):215–225. https://doi.org/10.1016/S0950-3293(01)00039-8

    Article  Google Scholar 

  • Tian Y, Wang W, Yuan C, Zhang L, Liu J, Liu J (2016) Nutritional and digestive properties of protein isolates extracted from the muscle of the common carp using pH shift processing. J Food Process Preserv 41(1):e12847. https://doi.org/10.1111/jfpp.12847

    Article  Google Scholar 

  • Undeland I, Kelleher SD, Hultin HO (2002) Recovery of functional proteins from herring (Clupea harengus) light muscle by an acid or alkaline solubilization process. J Agric Food Chem 50(25):7371–7379. https://doi.org/10.1021/jf020199u

    Article  CAS  Google Scholar 

  • WHO/FAO/UNU (2007) Protein and amino requirements in human nutrition. Report of a Joint WHO/FAO/UNU Expert Consultation, WHO Technical Report Series 935, World Health Organization, Geneva

    Google Scholar 

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Acknowledgements

Authors wish to express their sincere thanks to the Dean, College of Fisheries, GADVASU and University Authorities, GADVASU for their constant support during the work period. Authors also wish to express their thanks to Ms. Manvinder Kaur for her technical help during the preparation of manuscript.

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Authors and Affiliations

  1. Department of Harvest and Post-harvest Technology, College of Fisheries, GADVASU, Ludhiana, Punjab, 141004, India

    Vijay Kumar Reddy Surasani

  2. CSIR-Central Food Technological Research Institute, Mysore, Karnataka, India

    Tanaji Kudre & Rajashekhar V. Ballari

Authors
  1. Vijay Kumar Reddy Surasani
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  2. Tanaji Kudre
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  3. Rajashekhar V. Ballari
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Correspondence to Vijay Kumar Reddy Surasani.

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Responsible editor: Philippe Garrigues

Highlights

• Study was conducted to utilize pangas (Pangasius pangasius) processing waste (fillet frames) to recover proteins using pH shift method and to characterize the recovered isolates

• pH 2.0 from acidic range and pH 13.0 from alkaline range were found to have maximum protein solubility (p < 0.05).

• Acidic pH (pH 2.0) was found to have minimal effect on proteins, while alkaline pH (pH 13.0) caused protein denaturation resulting in less stable proteins and poor gel network.

• Both acidic and alkaline-aided processing caused significant (p < 0.05) reductions in total lipid, myoglobin, and pigment content thus by resulting in whiter proteins isolates and gels.

• The content of total essential amino acids increased during pH shift processing, indicating the enrichment of essential amino acids.

• No microbial counts were detected in isolates prepared using acid and alkaline extraction methods.

• pH shift processing was found to be promising in the utilization of fish processing waste for the recovery of functional proteins thus by reducing the supply demand gap as well pollution problems

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Surasani, V.K.R., Kudre, T. & Ballari, R.V. Recovery and characterization of proteins from pangas (Pangasius pangasius) processing waste obtained through pH shift processing. Environ Sci Pollut Res 25, 11987–11998 (2018). https://doi.org/10.1007/s11356-018-1456-x

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