Environmental Science and Pollution Research

, Volume 25, Issue 12, pp 11787–11799 | Cite as

Impact of oxytetracycline and bacterial bioaugmentation on the efficiency and microbial community structure of a pesticide-degrading biomixture

  • Víctor Castro-Gutiérrez
  • Mario Masís-Mora
  • Elizabeth Carazo-Rojas
  • Marielos Mora-López
  • Carlos E. Rodríguez-RodríguezEmail author
Research Article


An experimental study evaluating the effect of bioaugmentation and antibiotic (oxytetracycline) application on pesticide degradation and microbial community structure of a biomixture used in a biopurification system (BPR) was conducted. The bioaugmentation employed a carbofuran-degrading bacterial consortium. The non-bioaugmented biomixture showed excellent performance for removal of atrazine (t1/2: 9.9 days), carbendazim (t1/2: 3.0 days), carbofuran (t1/2: 2.8 days), and metalaxyl (t1/2: 2.7 days). Neither the addition of oxytetracycline nor bioaugmentation affected the efficiency of pesticide removal or microbial community (bacterial and fungal) structure, as determined by DGGE analysis. Instead, biomixture aging was mainly responsible for microbial population shifts. Even though the bioaugmentation did not enhance the biomixtures′ performance, this matrix showed a high capability to sustain initial stresses related to antibiotic addition; therefore, simultaneous elimination of this particular mixture of pesticides together with oxytetracycline residues is not discouraged.


Pesticides Biopurification systems Degradation DGGE Microbial communities 



This work was supported by Vicerrectoría de Investigación, Universidad de Costa Rica (project 802-B4-503), and the Costa Rican Ministry of Science, Technology and Telecommunications, MICITT (project FI-093-13).

Supplementary material

11356_2018_1436_MOESM1_ESM.docx (827 kb)
ESM 1 (DOCX 826 kb)


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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Centro de Investigación en Contaminación Ambiental (CICA)Universidad de Costa RicaSan JoséCosta Rica
  2. 2.Centro de Investigación en Biología Celular y Molecular (CIBCM)Universidad de Costa RicaSan JoséCosta Rica

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