Influence of land use on the health of a detritivorous fish (Ancistrus mullerae) endemic to the Iguassu ecoregion: relationship between agricultural land use and severe histopathological alterations

  • Mayara Pereira Neves
  • João Paulo de Arruda Amorim
  • Rosilene Luciana Delariva
Research Article

Abstract

This study aimed to evaluate the histopathological biomarkers of the gills and liver of endemic catfish to test the hypothesis that, in environments under intense land use by agricultural activities, histopathological alterations occur more severely. Samples were collected by electrofishing in seven streams in the Lower Iguaçu basin quarterly from August 2015 to February 2016. The gills and livers were processed according to routine histological methods and examined by light microscopy. The histopathological alterations observed in fish from the streams with a higher percentage of natural vegetation cover were considered modest and indicated normal functioning of the organ (such as edema, hyperplasia, and leukocyte infiltration). As predicted, fish collected in streams with higher agricultural influence presented moderate to severe damage (aneurysm, vacuolization and cytoplasmic degeneration, and pyknotic nucleus). The abundance of chloride cells was significantly increased in the gills of Ancistrus mullerae collected in rural streams. In addition, in most streams, mucous cells were more abundant during the rainy period. Significant differences were observed in the histopathological index (HI) of the gills and livers, where severe histopathological alterations occurred in fish from streams with a higher agricultural influence. The observed alterations were more severe in the liver than in the gills, which are indeed related to the liver’s key role in the detoxification of xenobiotics. We conclude that more severe histological alterations occurred in fish from streams with the highest land use by agricultural activities. Thus, our work provides important insight into the conservation and management of natural resources.

Keywords

Biomonitoring Loricariidae Histopathology Rural influence Preservation areas 

Notes

Acknowledgements

We thank Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES), Universidade Estadual do Oeste do Paraná (UNIOESTE), and to all our colleagues for their help in fieldwork and in the laboratory.

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  • Mayara Pereira Neves
    • 1
    • 2
  • João Paulo de Arruda Amorim
    • 3
  • Rosilene Luciana Delariva
    • 4
  1. 1.Programa de Pós-Graduação em Conservação e Manejo de Recursos Naturais, Centro de Ciências Biológicas e da SaúdeUniversidade Estadual do Oeste do ParanáCascavelBrazil
  2. 2.CCBS, Universidade Estadual do Oeste do ParanáCascavelBrazil
  3. 3.Centro de Ciências Biológicas e da SaúdeUniversidade Estadual do Oeste do ParanáCascavelBrazil
  4. 4.Centro de Ciências Biológicas e da Saúde, Programa de Pós-Graduação em Conservação e Manejo de Recursos NaturaisUniversidade Estadual do Oeste do ParanáCascavelBrazil

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