Environmental Science and Pollution Research

, Volume 25, Issue 10, pp 9730–9736 | Cite as

Use of statistical analysis to validate ecogenotoxicology findings arising from various comet assay components

  • Bilal Hussain
  • Tayyaba Sultana
  • Salma Sultana
  • Khalid Abdullah Al-Ghanim
  • Muhammad Shahreef Masoud
  • Shahid Mahboob
Research Article


Cirrhinus mrigala, Labeo rohita, and Catla catla are economically important fish for human consumption in Pakistan, but industrial and sewage pollution has drastically reduced their population in the River Chenab. Statistics are an important tool to analyze and interpret comet assay results. The specific aims of the study were to determine the DNA damage in Cirrhinus mrigala, Labeo rohita, and Catla catla due to chemical pollution and to assess the validity of statistical analyses to determine the viability of the comet assay for a possible use with these freshwater fish species as a good indicator of pollution load and habitat degradation. Comet assay results indicated a significant (P < 0.05) degree of DNA fragmentation in Cirrhinus mrigala followed by Labeo rohita and Catla catla in respect to comet head diameter, comet tail length, and % DNA damage. Regression analysis and correlation matrices conducted among the parameters of the comet assay affirmed the precision and the legitimacy of the results. The present study, therefore, strongly recommends that genotoxicological studies conduct appropriate analysis of the various components of comet assays to offer better interpretation of the assay data.


Ecotoxicology Major carps Heavy metals Comet assay Regression Correlation Outcomes 


Funding information

The authors (SM and KAAG) would like to express their sincere appreciation to the Deanship of Scientific Research at King Saud University for its funding of this research through the Research Group Project No. RG-1435-012.


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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Department of ZoologyGovernment College UniversityFaisalabadPakistan
  2. 2.Department of Zoology, College of ScienceKing Saud UniversityRiyadhSaudi Arabia
  3. 3.Department of Bioinformatics and BiotechnologyGovernment College UniversityFaisalabadPakistan

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