Environmental Science and Pollution Research

, Volume 25, Issue 10, pp 9443–9453 | Cite as

Generation of shrimp waste-based dispersant for oil spill response

  • Kedong Zhang
  • Baiyu Zhang
  • Xing Song
  • Bo Liu
  • Liang Jing
  • Bing Chen
Research Article


In this study, shrimp waste was enzymatically hydrolyzed to generate a green dispersant and the product was tested for crude oil dispersion in seawater. The hydrolysis process was first optimized based on the dispersant effectiveness (DE) of the product. The functional properties of the product were identified including stability, critical micelle concentration, and emulsification activity. Water was confirmed as a good solvent for dispersant generation when compared with three chemical solvents. The effects of salinity, mixing energy, and temperature on the dispersion of the Alaska North Slope (ANS) crude oil were examined. Microtox acute toxicity test was also conducted to evaluate the toxicity of the produced dispersant. In addition, DE of the product on three different types of crude oil, including ANS crude oil, Prudhoe Bay crude oil (PBC), and Arabian Light crude oil (ALC) was compared with that of the Corexit 9500, respectively. The research output could lead to a promising green solution to the oil spill problem and might result in many other environmental applications.


Shrimp waste Surfactant Dispersant Crude oil Renewable resources 



The authors would like to express the gratitude to Petroleum Research of Newfoundland and Labrador (PRNL) and Canada Foundation for Innovation (CFI) for its support.


  1. Adjonu R, Doran G, Torley P, Agboola S (2014) Formation of whey protein isolate hydrolysate stabilised nanoemulsion. Food Hydrocoll 41:169–177. CrossRefGoogle Scholar
  2. Aluko RE, Monu E (2003) Functional and bioactive properties of quinoa seed protein hydrolysates. J Food Sci 68(4):1254–1258. CrossRefGoogle Scholar
  3. Arboleda JC, Rojas OJ, Lucia LA (2014) Acid-generated soy protein hydrolysates and their interfacial behavior on model surfaces. Biomacromolecules 15(11):4336–4342. CrossRefGoogle Scholar
  4. Blondina GJ, Singer MM, Lee I, Ouano MT, Hodgins M, Tjeerdema RS, Sowby ML (1999) Influence of salinity on petroleum accommodation by dispersants. Spill Sci Technol Bull 5(2):127–134. CrossRefGoogle Scholar
  5. Cai L, Gochin M, Liu K (2011) A facile surfactant critical micelle concentration determination. Chem Commun 47(19):5527–5529. CrossRefGoogle Scholar
  6. Campisi T, Abbondanzi F, Casado-Martinez C, DelValls TA, Guerra R, Iacondini A (2005) Effect of sediment turbidity and color on light output measurement for Microtox® basic solid-phase test. Chemosphere 60(1):9–15. CrossRefGoogle Scholar
  7. Cao T (2015) Generation of biodispersants for offshore oil spill response (Master dissertation, Memorial University of Newfoundland)Google Scholar
  8. Carrillo PG, Mardaraz C, Pitta-Alvarez SI, Giulietti AM (1996) Isolation and selection of biosurfactant-producing bacteria. World J Microbiol Biotechnol 12(1):82–84. CrossRefGoogle Scholar
  9. Chalamaiah M, Rao GN, Rao DG, Jyothirmayi T (2010) Protein hydrolysates from meriga (Cirrhinus mrigala) egg and evaluation of their functional properties. Food Chem 120(3):652–657. CrossRefGoogle Scholar
  10. Chandrasekar S, Sorial GA, Weaver JW (2006) Dispersant effectiveness on oil spills–impact of salinity. J Mar Sci 63(8):1418–1430. Google Scholar
  11. Chang CY, Wu KC, Chiang SH (2007) Antioxidant properties and protein compositions of porcine haemoglobin hydrolysates. Food Chem 100(4):1537–1543. CrossRefGoogle Scholar
  12. Chapman H, Purnell K, Law RJ, Kirby MF (2007) The use of chemical dispersants to combat oil spills at sea: a review of practice and research needs in Europe. Mar Pollut Bull 54(7):827–838. CrossRefGoogle Scholar
  13. Cheng Y, Chen J, Xiong YL (2014) Interfacial adsorption of peptides in oil-in-water emulsions costabilized by tween 20 and antioxidative potato peptides. J Agric Food Chem 62(47):11575–11581. CrossRefGoogle Scholar
  14. de Castro RJS, Bagagli MP, Sato HH (2015) Improving the functional properties of milk proteins: focus on the specificities of proteolytic enzymes. Curr Opin Food Sci 1:64–69. CrossRefGoogle Scholar
  15. DeLeo DM, Ruiz-Ramos DV, Baums IB, Cordes EE (2016) Response of deep-water corals to oil and chemical dispersant exposure. Deep-Sea Res II Top Stud Oceanogr 129:137–147. CrossRefGoogle Scholar
  16. Dey SS, Dora KC (2014) Antioxidative activity of protein hydrolysate produced by alcalase hydrolysis from shrimp waste (Penaeus monodon and Penaeus indicus). J Food Sci Technol 51(3):449–457. CrossRefGoogle Scholar
  17. Fingas MF, Kyle DA, Holmes JB Tennyson EJ (1993). The effectiveness of dispersants: variation with energy. In International Oil Spill Conference. American Petroleum Institute. 1993(1) 567-574)
  18. Foegeding EA, Davis JP, Doucet D, McGuffey MK (2002) Advances in modifying and understanding whey protein functionality. Trends Food Sci Technol 13(5):151–159. CrossRefGoogle Scholar
  19. Gallert C, Winter J (2002) Solid and liquid residues as raw materials for biotechnology. Naturwissenschaften 89(11):483–496. CrossRefGoogle Scholar
  20. Gauthier SF, Pouliot Y (2003) Functional and biological properties of peptides obtained by enzymatic hydrolysis of whey proteins. J Dairy Sci 86:E78–E87. CrossRefGoogle Scholar
  21. George-Ares A, Clark JR (2000) Aquatic toxicity of two Corexit® dispersants. Chemosphere 40(8):897–906. CrossRefGoogle Scholar
  22. Gildberg A, Stenberg E (2001) A new process for advanced utilisation of shrimp waste. Process Biochem 36(8):809–812. CrossRefGoogle Scholar
  23. Gong Y, Zhao X, O'Reilly SE, Qian T, Zhao D (2014) Effects of oil dispersant and oil on sorption and desorption of phenanthrene with Gulf Coast marine sediments. Environ Pollut 185:240–249. CrossRefGoogle Scholar
  24. Goodbody-Gringley G, Wetzel DL, Gillon D, Pulster E, Miller A, Ritchie KB (2013) Toxicity of deepwater horizon source oil and the chemical dispersant, Corexit® 9500, to coral larvae. PLoS One 8(1):e45574. CrossRefGoogle Scholar
  25. Infante MR, Pérez L, Pinazo A, Clapés P, Morán MC, Angelet M et al (2004) Amino acid-based surfactants. C R Chim 7(6):583–592. CrossRefGoogle Scholar
  26. Innocente N, Corradini C, Blecker C, Paquot M (1998) Dynamic surface properties of the proteose-peptone fraction of bovine milk. J Dairy Sci 81(7):1833–1839Google Scholar
  27. Intarasirisawat R, Benjakul S, Visessanguan W, Wu J (2012) Antioxidative and functional properties of protein hydrolysate from defatted skipjack (Katsuwonous pelamis) roe. Food Chem 135(4):3039–3048. CrossRefGoogle Scholar
  28. Jamieson BL, Gonçalves AA, Gagnon GA (2013) Evaluation of treatment options for Atlantic Canadian seafood processing plant effluent. J Environ Eng Sci 8(4):448–460Google Scholar
  29. Judson RS, Martin MT, Reif DM, Houck KA, Knudsen TB, Rotroff DM, Xia M, Sakamuru S, Huang R, Shinn P, Austin CP, Kavlock RJ, Dix DJ (2010) Analysis of eight oil spill dispersants using rapid, in vitro tests for endocrine and other biological activity. Environ Sci Technol 44(15):5979–5985. CrossRefGoogle Scholar
  30. Kezwoń A, Chromińska I, Frączyk T, Wojciechowski K (2015) Effect of enzymatic hydrolysis on surface activity and surface rheology of type I collagen. Colloids Surf B: Biointerfaces 137:60–69. CrossRefGoogle Scholar
  31. Klompong V, Benjakul S, Kantachote D, Shahidi F (2007) Antioxidative activity and functional properties of protein hydrolysate of yellow stripe trevally (Selaroides leptolepis) as influenced by the degree of hydrolysis and enzyme type. Food Chem 102(4):1317–1327. CrossRefGoogle Scholar
  32. Korhonen H, Pihlanto A (2006) Bioactive peptides: production and functionality. Int Dairy J 16(9):945–960. CrossRefGoogle Scholar
  33. Kujawinski EB, Kido Soule MC, Valentine DL, Boysen AK, Longnecker K, Redmond MC (2011) Fate of dispersants associated with the deepwater horizon oil spill. Environ Sci Technol 45(4):1298–1306. CrossRefGoogle Scholar
  34. la Farré M, Garcı́a MJ, Tirapu L, Ginebreda A, Barceló D (2001) Wastewater toxicity screening of non-ionic surfactants by Toxalert® and Microtox® bioluminescence inhibition assays. Anal Chim Acta 427(2):181–189. CrossRefGoogle Scholar
  35. Lamsal BP, Koegel RG, Gunasekaran S (2007) Some physicochemical and functional properties of alfalfa soluble leaf proteins. Food Sci Technol 40(9):1520–1526. Google Scholar
  36. Leal ALG, de Castro PF, de Lima JPV, de Souza Correia E, de Souza Bezerra R (2010) Use of shrimp protein hydrolysate in Nile tilapia (Oreochromis niloticus, L.) feeds. Aquac Int 18(4):635–646. CrossRefGoogle Scholar
  37. Lessard RR, DeMarco G (2000) The significance of oil spill dispersants. Spill Sci Technol Bull 6(1):59–68. CrossRefGoogle Scholar
  38. Liu Q, Kong B, Xiong YL, Xia X (2010) Antioxidant activity and functional properties of porcine plasma protein hydrolysate as influenced by the degree of hydrolysis. Food Chem 118(2):403–410. CrossRefGoogle Scholar
  39. Mackay D, Chau A, Hossain K, Bobra M (1984) Measurement and prediction of the effectiveness of oil spill chemical dispersants. Oil Spill Chem Dispersants Res Experience Recomm ASTM STP 840:38–54. CrossRefGoogle Scholar
  40. Mizani MARYAM, Aminlari M, Khodabandeh M (2005) An effective method for producing a nutritive protein extract powder from shrimp-head waste. Food Sci Technol Int 11(1):49–54. CrossRefGoogle Scholar
  41. Moles A, Holland L, Short J (2002) Effectiveness in the laboratory of Corexit 9527 and 9500 in dispersing fresh, weathered, and emulsion of Alaska north slope crude oil under subarctic conditions. Spill Sci Technol Bull 7(5):241–247. CrossRefGoogle Scholar
  42. Muheem A, Shakeel F, Jahangir MA, Anwar M, Mallick N, Jain GK, Warsi MH, Ahmad FJ (2014) A review on the strategies for oral delivery of proteins and peptides and their clinical perspectives. Saudi Pharm J 24(4):413–428. CrossRefGoogle Scholar
  43. Pietroski JP, White JR, DeLaune RD (2015) Effects of dispersant used for oil spill remediation on nitrogen cycling in Louisiana coastal salt marsh soil. Chemosphere 119:562–567. CrossRefGoogle Scholar
  44. Plascencia-Jatomea M, Olvera-Novoa MA, Arredondo-Figueroa JL, Hall GM, Shirai K (2002) Feasibility of fishmeal replacement by shrimp head silage protein hydrolysate in Nile tilapia (Oreochromis niloticus L) diets. J Sci Food Agric 82(7):753–759. CrossRefGoogle Scholar
  45. Prince RC, Butler JD (2014) A protocol for assessing the effectiveness of oil spill dispersants in stimulating the biodegradation of oil. Environ Sci Pollut Res 21(16):9506–9510. CrossRefGoogle Scholar
  46. Saeki H, Sasaki M, Komatsu K, Miura A, Matsuda H (2009) Oil spill remediation by using the remediation agent JE1058BS that contains a biosurfactant produced by Gordonia sp. strain JE-1058. Bioresour Technol 100(2):572–577. CrossRefGoogle Scholar
  47. Šližytė R, Mozuraitytė R, Martinez-Alvarez O, Falch E, Fouchereau-Peron M, Rustad T (2009) Functional, bioactive and antioxidative properties of hydrolysates obtained from cod (Gadus morhua) backbones. Process Biochem 44(6):668–677. CrossRefGoogle Scholar
  48. Sorial GA, Venosa AD, Koran KM, Holder E, King DW (2004) Oil spill dispersant effectiveness protocol. II: performance of revised protocol. J Environ Eng 130(10):1085–1093. CrossRefGoogle Scholar
  49. Srinivasan R, Lu Q, Sorial GA, Venosa AD, Mullin J (2007) Dispersant effectiveness of heavy fuel oils using baffled flask test. Environ Eng Sci 24(9):1307–1320. CrossRefGoogle Scholar
  50. Synowiecki J, Al-Khateeb NAAQ (2000) The recovery of protein hydrolysate during enzymatic isolation of chitin from shrimp Crangon crangon processing discards. Food Chem 68(2):147–152. CrossRefGoogle Scholar
  51. Tang L, Sun J, Zhang HC, Zhang CS, Yu LN, Bi J, Zhu F, Liu SF, Yang QL (2012) Evaluation of physicochemical and antioxidant properties of peanut protein hydrolysate. PLoS One 7(5):e37863. CrossRefGoogle Scholar
  52. Venosa AD, King DW, Sorial GA (2002) The baffled flask test for DE: a round robin evaluation of reproducibility and repeatability. Spill Science & Technology Bulletin 7(5):299–308. CrossRefGoogle Scholar
  53. Zhang K, Zhang B, Chen B, Jing L, Zhu Z, Kazemi K (2016) Modeling and optimization of Newfoundland shrimp waste hydrolysis for microbial growth using response surface methodology and artificial neural networks. Mar Pollut Bull 109(1):245–252. CrossRefGoogle Scholar
  54. Zhao J, Xiong YL (2015) Interfacial peptide partitioning and undiminished antioxidative and emulsifying activity of oxidatively stressed soy protein hydrolysate in an O/W emulsion. Food Sci Technol 61(2):322–329. Google Scholar
  55. Zhao J, Xiong YL, McNear DH (2013) Changes in structural characteristics of antioxidative soy protein hydrolysates resulting from scavenging of hydroxyl radicals. J Food Sci 78(2):C152–C159. CrossRefGoogle Scholar

Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  • Kedong Zhang
    • 1
  • Baiyu Zhang
    • 1
  • Xing Song
    • 1
  • Bo Liu
    • 1
  • Liang Jing
    • 1
  • Bing Chen
    • 1
  1. 1.Faculty of Engineering and Applied ScienceMemorial UniversitySt. John’sCanada

Personalised recommendations