Abstract
The trace metal cadmium (Cd) is a toxic pollutant known to induce oxidative stress and other sublethal to lethal effects on aquatic organisms. We exposed the marine mud shrimp Austinogebia edulis to Cd concentrations of 0, 0.5, and 5 mg/kg for up to 4 days (24, 48, 72, 96 h). We studied the activity of antioxidant enzymes in the hepatopancreas, gill, and muscle of A. edulis. Antioxidant enzymes (SOD, CAT, and GPx) decreased with increasing Cd concentration and extended exposure time in these three organs of the mud shrimp A. edulis. Increasing Cd concentration led to an increase in ROS and resulted ultimately in membrane lipid peroxidation at higher Cd concentrations. Significant damage of the hepatopancreas of A. edulis was noticed at higher concentrations of Cd, showing damages like the disappearance of epithelial cell boundaries, detachment of cells from the basal lamina, cellular swelling, necrosis, and reduction of glycogen. In conclusion, Cd caused oxidative damage by reducing the activities of antioxidant enzymes and by damaging the tissue structure in major organs of the mud shrimp A. edulis.
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Acknowledgements
We thank the College of Life Science of Shanxi University, China, for providing the necessary facilities and equipment for carrying out this study. Finally, we thank Mr. Chih-Ming Lin and Dr. Shao-Hung Peng for their assistance during the field sampling. This paper is a contribution to the International Joint Laboratory between Lille University and Taiwan.
Funding
We received from the Ministry of Science and Technology (MOST) of Taiwan financial support through grant nos. MOST 104-2611-M-019- 004, MOST 105-2621-M-019-001, MOST 105-2918-I-019-001 and MOST 106-2621-M-019- 001, and MOST 107-2621-M-019-001 to J.-S. Hwang, as well as the grant nos. MOST 104-2811-M-019-005, MOST 105-2811-M-019-008, MOST 106-2811-M-019-004, and MOST 107-2811-M-019-004 to L.-C. Tseng.
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Highlights
• Cd lowered antioxidant enzyme activities in all tissues of A. edulis.
• Increased ROS resulted in membrane lipid peroxidation (MDA).
• Cd induced structural changes in the hepatopancreas.
• Hepatopancreas decreased mucus and glycoprotein production after Cd exposure.
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Das, S., Tseng, LC., Chou, C. et al. Effects of cadmium exposure on antioxidant enzymes and histological changes in the mud shrimp Austinogebia edulis (Crustacea: Decapoda). Environ Sci Pollut Res 26, 7752–7762 (2019). https://doi.org/10.1007/s11356-018-04113-x
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DOI: https://doi.org/10.1007/s11356-018-04113-x