Abstract
Ozone helps decontamination environments due to its oxidative power, however present toxicity when it is in high concentrations, by long periods of exposition. This study aimed to assess the safety of ozone generator air purifier at concentrations of 0.05 ppm in rats exposed to 3 and 24 h/day for 14 and 28 days. No significant differences are observed between groups in clinical signs, feed and water intake, relative body weight gain and relative weight of organs, macroscopy and microscopy of lungs, and oxidative plasma assay. In this exposure regime, ozone does not cause genotoxicity and no significant changes in pulmonary histology indicative of toxicity. Ozone generated in low concentrations, even in exposure regimes above the recommended is safe, both acute and sub-acute exposition.
Similar content being viewed by others
Explore related subjects
Discover the latest articles, news and stories from top researchers in related subjects.References
Alexis NE, Becker S, Bromberg PA et al (2004) Circulating CD11b expression correlates with the neutrophil response and airway mCD14 expression is enhanced following ozone exposure in humans. Clin Immunol 111:126–131. doi:10.1016/j.clim.2003.12.002
Anenberg SC, Horowitz LW, Tong DQ, West JJ (2010) An estimate of the global burden of anthropogenic ozone and fine particulate matter on premature human mortality using atmospheric modeling. Environ Health Perspect 118:1189–1196. doi:10.1289/ehp.0901220
Aris RM, Christian D, Hearne PQ et al (1993) Ozone-induced airway inflammation in human subjects as determined by airway lavage and biopsy. Am Rev Respir Dis 148:1363–1372
Bell ML, McDermott A, Zeger SL et al (2004) Ozone and short-term mortality in 95 US urban communities, 1987–2000. JAMA 292:2372–2378. doi:10.1001/jama.292.19.2372
Bell ML, Zanobetti A, Dominici F (2014) Who is more affected by ozone pollution? A systematic review and meta-analysis. Am J Epidemiol 180:15–28. doi:10.1093/aje/kwu115
Bertol CD, Vieira KP, Rossato LG, D’ Avila JV (2012) Microbiological environmental monitoring after the use of air purifier ozone generator. Ozone Sci Eng J Int Ozone Assoc 34:225–230. doi:10.1080/01919512.2012.679871
Bhalla DK, Gupta SK (2000) Lung injury, inflammation, and inflammatory stimuli in rats exposed to ozone. J Toxicol Environ Health 59:211–228. doi:10.1080/009841000156899
Bracht A, Ishii-Iwamoto EL (2003) Métodos de Laboratório em Bioquímica, Manole, São Paulo, 275–288
Brasil (2016) Ministério do trabalho e Emprego. Norma Regulamentadora (NR) 15, Anexo 11: Atividades e Operações Insalubres. <http://www.mte.gov.br/legislacao/normas_regulamentadoras/nr_15_anexo11.pdf>. Accessed 27 Apr 2014
Brasil (2008) Lei n. 11.794, de 8 de outubro de 2008. Regulamenta o inciso VII do § 1o do art. 225 da Constituição Federal, estabelecendo procedimentos para o uso científico de animais; revoga a Lei n. 6.638, de 8 de maio de 1979; e dá outras providências
Burge PS (2004) Sick building syndrome. Occup Environ Med 61:185–190. doi:10.1136/oem.2003.008813
Chang L, Miller FJ, Ultman J et al (1991) Alveolar epithelial cell injuries by subchronic exposure to low concentrations of ozone correlate with cumulative exposure. Toxicol Appl Pharmacol 109:219–234. doi:10.1016/0041-008X(91)90170-J
Chen CH, Chan CC, Chen BY et al (2015) Effects of particulate air pollution and ozone on lung function in non-asthmatic children. Environ Res 137:40–48. doi:10.1016/j.envres.2014.11.021
Choy WN (2001) Regulatory genetic toxicology tests. In: Genetic toxicology and cancer risk assessment. Marcel Dekker, New York, pp 93–113
Collins AR (2004) The comet assay for DNA damage and repair: principles, applications, and limitations. Mol Biotechnol 26:249–261
Crapo JD (1986) Morphologic changes in pulmonary oxygen toxicity. Ann Rev Physiol 48:721–731
Da Silva J, TRO DF, Heuser V et al (2000) Genotoxicity biomonitoring in coal regions using wild rodent Ctenomys torquatus by Comet assay and micronucleus test. Environ Mol Mutagen 35:270–278. doi:10.1002/1098–2280(2000)35:4<270::AID-EM2>3.0.CO;2-L
Devlin RB, WF MD, Mann R et al (1991) Exposure of humans to ambient levels of ozone for 6.6 hours causes cellular and biochemical changes in the lung. Am J Respir Cell Mol Biol 4:72–81. doi:10.1165/ajrcmb/4.1.72
Devlin RB, Raub JA, Folinsbee LJ (1997) Health Effects of Ozone. Sci Med 4:8–17
Ellman GL (1959) Tissue sulfhydryl groups. Arch Biochem Biophys 82:70–77
Goudarzia G, Zallaghic E, Neissia A et al (2013) Cardiopulmonary mortalities and chronic obstructive pulmonary disease attributed to ozone air pollution. Arch Hyg Sci 2:62–72
Hellman B, Vaghef H, Boström B (1995) The concepts of tail moment and tail inertia in the single cell gel electrophoresis assay. Mutat Res Repair 336:123–131. doi:10.1016/0921–8777(94)00047-A
Hudson JB, Sharma M, Petric M (2007) Inactivation of Norovirus by ozone gas in conditions relevant to healthcare. J Hosp Infect 66:40–45. doi:10.1016/j.jhin.2006.12.021
IEC (2002) Reference number IEC 60335–2–65:2002(E). IEC- Int Electrotech Comm 2002:1–19. Availabe at: http://webstore.iec.ch/preview/info_iec60335-2-65%7Bed2.0%7Den.pdf. Access 08–08–2011
Krishna MT, Madden J, Teran LM, et al (1998) Effects of 0. 2 ppm ozone on biomarkers of inflammation in bron- choalveolar lavage fluid and bronchial mucosa of healthy subjects. Eur Respir J 11:1294–1300. doi: 10.1183/09031936.98.11061294
Lee SD, Mustafa MD, Mehlman MA (1983) The biomedical effects of ozone and related photochemical oxidants. Princeton Scientific Pub, Princeton
Leslie KO, Wick MR (2011) Practical pulmonary pathology: a diagnostic approach. Elsevier Health Sci
Lin YC, Juan HC, Cheng YC (2007) Ozone exposure in the culture medium inhibits enterovirus 71 virus replication and modulates cytokine production in rhabdomyosarcoma cells. Antivir Res 76:241–251. doi:10.1016/j.antiviral.2007.07.004
Long NC, Suh J, Morrow JD et al (2001) Ozone causes lipid peroxidation but little antioxidant depletion in exercising and nonexercising hamsters. J Appl Physiol 91:1694–1700
Lowry OH, Rosebrough NJ, Farr L, Randall RJ (1951) Protein measurement with the Folin phenol reagent. J Biol Chem 193:265–275
Mehlman MA, Borek C (1987) Toxicity and biochemical mechanisms of ozone. Environ Res 42:36–53. doi:10.1016/S0013-9351(87)80005-1
Murray BK, Ohmine S, Tomer DP et al (2008) Virion disruption by ozone-mediated reactive oxygen species. J Virol Methods 153:74–77. doi:10.1016/j.jviromet.2008.06.004
Mustafa MG (1990) Biochemical basis of ozone toxicity. Free Radic Biol Med 9:245–265. doi:10.1016/0891-5849(90)90035-H
Ohkawa H, Ohishi N, Yagi K (1979) Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal Biochem 95:351–358. doi:10.1016/0003-2697(79)90738-3
OSHA (1992) Occupational safety & health administration; U.S. Department of Health and Human Services; survey report: control technology for autobody repair and painting shops. <https://www.osha.gov/dts/chemicalsampling/data/CH_259300.html>. Accessed 27 Apr 2015
Peden DB, Setzer RW, Devlin RB (1995) Ozone exposure has both a priming effect on allergen-induced responses and an intrinsic inflammatory action in the nasal airways of perennially allergic asthmatics. Am J Respir Crit Care Med 151:1336–1345. doi:10.1164/ajrccm.151.5.7735583
Petry G, Rossato LG, Nespolo J et al (2014) In vitro inactivation of herpes virus by ozone. Ozone Sci Eng J Int Ozone Assoc 36:249–252. doi:10.1080/01919512.2013.862165
Robbins SL, Cotrans RS (2010) Bases patológicas das doenças [tradução FERNANDES, P. D.; et al.]. Elsevier, Rio de Janeiro, pg 46–57
Ruidavets J-B, Cournot M, Cassadou S et al (2005) Ozone air pollution is associated with acute myocardial infarction. Circulation 111:563–569. doi:10.1161/01.CIR.0000154546.32135.6E
Sasaki YF, Nakamura T, Kawaguchi S (2007) What is better experimental design for in vitro comet assay to detect chemical genotoxicity? Jap Soc Altern Anim Exp:499–504
SBCAL (2016) SBCAL - Sociedade Brasileira de Ciência em Animais de Laboratório http://www.cobea.org.br/. Accessed 17 Aug 2014
Silva RA, West JJ, Zhang Y et al (2013) Global premature mortality due to anthropogenic outdoor air pollution and the contribution of past climate change. Environ Res Lett 8:1–11. doi:10.1088/1748-9326/8/3/034005
Silverman RA, Ito K (2010) Age-related association of fine particles and ozone with severe acute asthma in New York City. J Allergy Clin Immunol 125:367–373. doi:10.1016/j.jaci.2009.10.061
Tager IB, Balmes J, Lurmann F et al (2005) Chronic exposure to ambient ozone and lung function in young adults. Epidemiology 16:751–759. doi:10.1097/01.ede.0000183166.68809.b0
Thurston-Enriquez JA, Haas CN, Jacangelo J, Gerba CP (2005) Inactivation of enteric adenovirus and feline calicivirus by ozone. Water Res 39:3650–3656. doi:10.1016/j.watres.2005.06.006
Toivola M, Alm S, Nevalainen A (2004) Viable fungi and bacteria in personal exposure samples in relation to microenvironments. J Environ Monit 6:113–120. doi:10.1039/b309960a
Viebahn-Hänsler R, León Fernández OS, Fahmy Z (2012) Ozone in medicine: the low-dose ozone concept-guidelines and treatment strategies. Ozone Sci Eng J Int Ozone Assoc 34:408–424. doi:10.1080/01919512.2012.717847
Wallace L (1996) Indoor particles: a review. J Air Waste Manage Assoc 46:98–126
WHO (2010) World Health Organization- WHO guidelines for indoor air quality: selected pollutants. WHO Guidel 9:1–454. doi:10.1186/2041-1480-2-S2-I1
WHO (1979) Photochemical oxidants: environmental health criteria 7. In: World Heal. Organ. <http://www.inchem.org/documents/ehc/ehc/ehc007.htm>. Accessed 25 Jun 2013
Author information
Authors and Affiliations
Corresponding author
Additional information
Responsible editor: Philippe Garrigues
Rights and permissions
About this article
Cite this article
Cestonaro, L.V., Marcolan, A.M., Rossato-Grando, L.G. et al. Ozone generated by air purifier in low concentrations: friend or foe?. Environ Sci Pollut Res 24, 22673–22678 (2017). https://doi.org/10.1007/s11356-017-9887-3
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s11356-017-9887-3