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Environmental Science and Pollution Research

, Volume 24, Issue 27, pp 21693–21699 | Cite as

Anxiety-like behavioural effects of extremely low-frequency electromagnetic field in rats

  • Natasa Z. Djordjevic
  • Milica G. Paunović
  • Aleksandar S. Peulić
Research Article

Abstract

In recent years, extremely low-frequency electromagnetic field (ELF-EMF) has received considerable attention for its potential biological effects. Numerous studies have shown the role of ELF-EMF in behaviour modulation. The aim of this study was to investigate the effect of short-term ELF-EMF (50 Hz) in the development of anxiety-like behaviour in rats through change hypothalamic oxidative stress and NO. Ten adult male rats (Wistar albino) were divided in two groups: control group—without exposure to ELF-EMF and experimental group—exposed to ELF-EMF during 7 days. After the exposure, time open field test and elevated plus maze were used to evaluate the anxiety-like behaviour of rats. Upon completion of the behavioural tests, concentrations of superoxide anion (O2·), nitrite (NO2 , as an indicator of NO) and peroxynitrite (ONOO) were determined in the hypothalamus of the animals. Obtained results show that ELF-EMF both induces anxiety-like behaviour and increases concentrations of O2· and NO, whereas it did not effect on ONOO concentration in hypothalamus of rats. In conclusion, the development of anxiety-like behaviour is mediated by oxidative stress and increased NO concentration in hypothalamus of rats exposed to ELF-EMF during 7 days.

Keywords

Electromagnetic field Anxiety Nitric oxide Oxidative stress Hypothalamus 

Notes

Acknowledgments

This study was supported by the Ministry of Education, Science and Technological Development of Republic of Serbia, Grant No. 173041. Authors are grateful to Jovan Vukovic (MSc in biology) and Vojkan Cvetković (MSc in biology) for technical assistance during the experiment.

Compliance with ethical standards

All experimental procedures were approved by the Faculty Ethics Committee, University of Kragujevac.

Conflict of interest

The authors declare that they have no conflicts of interest.

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Copyright information

© Springer-Verlag GmbH Germany 2017

Authors and Affiliations

  • Natasa Z. Djordjevic
    • 1
  • Milica G. Paunović
    • 2
  • Aleksandar S. Peulić
    • 3
  1. 1.Department of Biomedical SciencesState University of Novi PazarNovi PazarSerbia
  2. 2.Institute of Biology and Ecology, Faculty of ScienceUniversity of KragujevacKragujevacSerbia
  3. 3.Faculty of engineeringUniversity of KragujevacKragujevacSerbia

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