Physiological and molecular responses of the earthworm Eisenia fetida to polychlorinated biphenyl contamination in soil
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Polychlorinated biphenyls (PCBs) are a class of man-made organic compounds ubiquitously present in the biosphere. In this study, we evaluated the toxic effects of different concentrations of PCBs in two natural soils (i.e. red soil and fluvo-aquic soil) on the earthworm Eisenia fetida. The parameters investigated included anti-oxidative response, genotoxic potential, weight variation and biochemical responses of the earthworm exposed to two different types of soils spiked with PCBs after 7 or 14 days of exposure. Earthworms had significantly lower weights in both soils after PCB exposure. PCBs significantly increased catalase (CAT), superoxide dismutase (SOD), and guaiacol peroxidase (POD) activity in earthworms exposed to either soil type for 7 or 14 days and decreased the malondialdehyde (MDA) content in earthworms exposed to red soil for 14 days. Of the enzymes examined, SOD activity was the most sensitive to PCB stress. In addition, PCB exposure triggered dose-dependent coelomocyte DNA damage, even at the lowest concentration tested. This response was relatively stable between different soils. Three-way analysis of variance (ANOVA) showed that the weight variation, anti-oxidant enzyme activities, and MDA contents were significantly correlated with exposure concentration or exposure duration (P < 0.01). Furthermore, weight variation, CAT activity, and SOD activity were significantly affected by soil type (P < 0.01). Therefore, the soil type and exposure time influence the toxic effects of PCBs, and these factors should be considered when selecting responsive biomarkers.
KeywordsAcute toxicity Anti-oxidant enzymes Comet assay Eisenia fetida Malondialdehyde Polychlorinated biphenyls
This study was financially supported by the National Natural Science Foundation (No. 41371469), the Fundamental Research Funds for the Central Universities (KYZ201626), Special Fund for Agro-scientific Research in the Public Interest (No. 201503121-01), China Postdoctoral Science Foundation Funded Project (No. 2016M591856), Anhui Postdoctoral Science Foundation Funded Project (No. 2015B057), Science and Technology Project of Anhui Province (1604a0702006), and the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD). We are grateful to the anonymous reviewers for their valuable comments and Ms. Kathleen Farquharson for the language improvement.
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Conflict of interest
The authors declare that they have no conflict of interest.
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