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Detection of typhoid fever by diatom-based optical biosensor

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Surface-modified diatom substrates are employed for the development of immunocomplex-based optical biosensor for diagnosis of typhoid. Biosensor has been prepared by covalent immobilization of Salmonella typhi antibody onto the crosslinked diatom substrates via glutaraldehyde. Photoluminescent (PL) studies revealed good specificity and ability of conjugated diatom substrates to distinguish complementary (S. typhi) and non-complementary (Escherichia coli) antigens. The immunocomplexed biosensor showed detection limit of 10 pg. The excellent performance of biosensor is associated to its large surface-to-volume ratio, good photoluminescent property, and biocompatibility of diatom frustules, which enhances the antibody immobilization and facilitates the nucleophilic electron transfer between antibody and conjugated diatom surface. Hence, immunocomplexed diatom substrates are considered to be a suitable platform for the environmental monitoring of water-borne pathogen S. typhi.

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  • Betard A, Fischer RA (2012) Metal organic framework substrates: from fundamentals to applications. Chem Rev 112:1055–1083

    Article  CAS  Google Scholar 

  • De Stefano L, Rendina I, De Stefano M, Bismuto A, Maddalena P (2005) Marine diatoms as optical chemical sensors. Appl Phys Lett 87:233902

    Article  CAS  Google Scholar 

  • De Stefano L, Lamberti A, Rotiroti L, De Stefano M (2008) Interfacing the nanostructured biosilica microshells of the marine diatom Coscinodiscus wailesii with biological matter. Acta Biomater 4:126–130

    Article  CAS  Google Scholar 

  • De Stefano L, Rotiroti L, Lamberti A, Lettieri S, Setaro A, De Stefano M, Maddalena P (2009) Marine diatoms as optical biosensors. Biosens Bioelectron 24:1580–1584

    Article  CAS  Google Scholar 

  • Gale DK, Gutu T, Jiao J, Chang C-H, Rorrer GL (2009) Photoluminescence detection of biomolecules by antibody-functionalized diatom biosilica. Adv Funct Mater 19:926–933

    Article  CAS  Google Scholar 

  • Goswami B, Choudhury A, Buragohain AK (2012) Luminescence properties of a nanoporous freshwater diatom. Luminescence 27:16–19

    Article  CAS  Google Scholar 

  • Han D, Yan L, Chen W, Li W (2011) Preparation of chitosan/graphene oxide composite film with enhanced mechanical strength in the wet state. Carbohydr Polym 83:653–658

    Article  CAS  Google Scholar 

  • Lettieri S, Setaro A, De Stefano L, De Stefano M, Maddalena P (2008) The gas-detection properties of light-emitting diatoms. Adv Funct Mater 18:1257–1264

    Article  CAS  Google Scholar 

  • Li B, Shan C-L, Zhou Q, Fang Y, Wang Y-L, Xu F, Han L-R, Ibrahim M, Guo L-B, Xie G-L, Sun G-C (2013) Synthesis, characterization, and antibacterial activity of cross-linked chitosan-glutaraldehyde. Mar Drugs 11:1534–1552

    Article  CAS  Google Scholar 

  • Mansur HS, Sadahira CM, Souza AN, Mansur AAP (2008) FTIR spectroscopy characterization of poly(vinyl alcohol) hydrogel with different hydrolysis degree and chemically crosslinked with glutaraldehyde. Mater Sci Engg C 28:539–548

    Article  CAS  Google Scholar 

  • Pui CF, Wong WC, Chai LC, Tunung R, Jeyaletchumi P, Noor Hidayah MS, Ubong A, Farinazleen MG, Cheah YK, Son R (2011) Salmonella: a foodborne pathogen. Int Food Res J 18:465–473

    Google Scholar 

  • Qin GG, Jia YQ (1993) Mechanism of the visible luminescence in porous silicon. Solid State Commun 86:559-563

  • Sandeep KV, Edmond L, Sabahudin H, Keith BM, John HTL (2014) Immobilization of antibodies and enzymes on 3-aminopropyltriethoxysilane-functionalized bioanalytical platforms for biosensors and diagnostics. Chem Rev 114:11083–11130

    Article  CAS  Google Scholar 

  • Sarangi SN, Goswami K, Sahu SN (2007) Biomolecular recognition in DNA tagged CdSe nanowires. Biosens Bioelectron 22:3086–3091

    Article  CAS  Google Scholar 

  • Sharan R, Chhibber S, Reed RH (2011) Inactivation and sub-lethal injury of Salmonella typhi, Salmonella typhimurium and Vibrio cholerae in copper water storage vessels. BMC Infect Dis 11:1–6

    Article  CAS  Google Scholar 

  • Singh A, Singh MP, Sharma V, Verma HN, Arora K (2012) Chemical analysis of food: techniques and applications. Elsevier, Amsterdam, pp 407–464

    Book  Google Scholar 

  • Viji S, Anbazhagi M, Ponpandian N, Mangalaraj D, Jeyanthi S, Santhanam P, Shenbaga Devi A, Viswanathan C (2014) Diatom-based label-free optical biosensor for biomolecules. Appl Biochem Biotechnol 174:1173–1166

    Article  CAS  Google Scholar 

  • Yu Y, Addai-Mensah J, Losic D (2010) Synthesis of self-supporting gold microstructures with three-dimensional morphologies by direct replication of diatom templates. Langmuir 26:14068–14072

    Article  CAS  Google Scholar 

  • Zhang XL, Jeza VT, Pan Q (2008) Salmonella typhi: from a human pathogen to a vaccine vector. Cell Mol Immunol 5:91–97

    Article  CAS  Google Scholar 

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The authors thank Dr. P. Santhanam, Marine Planktonology & Aquaculture Lab, Department of Marine Science, Bharathidasan University, Tiruchirappalli, Tamilnadu, India, for providing diatom sample for this work.

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Correspondence to Viswanathan Chinnuswamy.

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The authors declare no potential conflicts of interest with respect to the authorship and/or publication of this article.

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Responsible editor: Philippe Garrigues

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Selvaraj, V., Muthukumar, A., Nagamony, P. et al. Detection of typhoid fever by diatom-based optical biosensor. Environ Sci Pollut Res 25, 20385–20390 (2018).

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