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Green biotransformations catalysed by enzyme-inorganic hybrid nanoflowers in environmentally friendly ionic solvents

  • Athena A. Papadopoulou
  • Andromachi Tzani
  • Angeliki C. Polydera
  • Petros Katapodis
  • Epaminondas Voutsas
  • Anastasia Detsi
  • Haralambos Stamatis
Protection and Restoration of the Environment

Abstract

Environmentally friendly ionic solvents such as (a) ionic liquids (ILs) formulated with hydroxyl ammonium cations and various carboxylic acid anions and (b) choline chloride or ethyl ammonium chloride-based deep eutectic solvents (DES) were tested as media for hydrolytic and synthetic reactions catalysed by lipase-inorganic hybrid nanoflowers. The nature of ionic solvents used has a significant effect on the hydrolytic and synthetic activity of the immobilized lipase, as well as on its stability and reusability. In choline chloride-based DES, the activity and especially the operational stability of the biocatalyst are significantly increased compared to those observed in buffer, indicating the potential application of these solvents as green media for various biocatalytic processes of industrial interest.

Keywords

Green solvents Lipase Hybrid nanoflowers Ionic liquids Deep eutectic solvents 

Notes

Acknowledgements

A.T. gratefully acknowledges financial support from the Research Committee of the National Technical University of Athens (scholarship for postgraduate studies).

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Copyright information

© Springer-Verlag Berlin Heidelberg 2017

Authors and Affiliations

  • Athena A. Papadopoulou
    • 1
  • Andromachi Tzani
    • 2
  • Angeliki C. Polydera
    • 1
  • Petros Katapodis
    • 1
  • Epaminondas Voutsas
    • 2
  • Anastasia Detsi
    • 2
  • Haralambos Stamatis
    • 1
  1. 1.Department of Biological Applications & Technologies, Laboratory of BiotechnologyUniversity of IoanninaIoanninaGreece
  2. 2.Laboratory of Organic Chemistry, School of Chemical EngineeringNational Technical University of AthensAthensGreece

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